Bicarbonate Activation of Monomeric Photosystem II-PsbS/Psb27 Complex

In thylakoid membranes, Photosystem II monomers from the stromal lamellae contain the subunits PsbS and Psb27 (PSIIm-S/27), while Photosystem II monomers from granal regions (PSIIm) lack these subunits. Here, we have isolated and characterised these two types of Photosystem II complexes. The PSIIm-S/27 showed enhanced fluorescence, the near-absence of oxygen evolution, as well as limited and slow electron transfer from QA to QB compared to the near-normal activities in the granal PSIIm. However, when bicarbonate was added to the PSIIm-S/27, water splitting and QA to QB electron transfer rates were comparable to those in granal PSIIm. The findings suggest that the binding of PsbS and/or Psb27 inhibits forward electron transfer and lowers the binding affinity for the bicarbonate. This can be rationalized in terms of the recently discovered photoprotection role played by bicarbonate binding via the redox tuning of the QA/QA?- couple, which controls the charge recombination route, and this limits chlorophyll triplet mediated 1O2 formation (Brinkert K et al. (2016) Proc Natl Acad Sci U S A. 113(43):12144-12149). These findings suggest that PSIIm-S/27 is an intermediate in the assembly of PSII in which PsbS and/or Psb27 restrict PSII activity while in transit, by using a bicarbonate-mediated switch and protective mechanism.

Metastasiertes Plattenepithelkarzinom auf einem Ulkus bei Graft-versus-Host-Disease nach allogener Stammzelltransplantation

ZusammenfassungWir berichten über einen 48-jährigen multimorbiden Patienten, der vor 26 Jahren eine allogene Knochenmarktransplantation aufgrund einer chronischen myeloischen Leukämie erhielt; 24 Jahre lang litt der Patient an einer sklerodermiformen chronischen Graft-versus-Host-Disease (GVHD) der Haut und der Lunge mit partieller Lungenresektion und immunsuppressiver Therapie. An den Unterschenkeln entwickelten sich rezidivierende Ulzerationen an den von der kutanen GVHD betroffenen Stellen. Der Patient stellte sich mit einem größenprogredienten Ulkus mit Therapieresistenz in unserer Klinik vor. Histologisch konnte ein Plattenepithelkarzinom diagnostiziert werden. Die Magnetresonanztomographie zeigte eine Knochenbeteiligung und eine kutane In-Transit-Metastase, und die Computertomographie ergab eine Metastase im Os sacrum. Bevor die Therapie eingeleitet wurde, verstarb der Patient plötzlich an den Folgen seiner Vorerkrankungen. Die Entwicklung einer kutanen GVHD ist häufig bei Patienten mit allogener Stammzelltransplantation. Hierbei ist das Risiko für die Entwicklung von Plattenepithelkarzinomen erhöht. Patienten sollten unter engmaschiger dermatologischer Kontrolle stehen. Bei Verdacht auf ein Plattenepithelkarzinom bei vorbestehender GVHD sollte zeitnah eine bioptische Sicherung erfolgen, um das Risiko einer Metastasierung zu senken.

KELT-9 as an Eclipsing Double-lined Spectroscopic Binary: A Unique and Self-consistent Solution to the System

Transiting hot Jupiters present a unique opportunity to measure absolute planetary masses due to the magnitude of their radial velocity signals and known orbital inclination. Measuring planet mass is critical to understanding atmospheric dynamics and escape under extreme stellar irradiation. Here we present the ultrahot Jupiter system KELT-9 as a double-lined spectroscopic binary. This allows us to directly and empirically constrain the mass of the star and its planetary companion without reference to any theoretical stellar evolutionary models or empirical stellar scaling relations. Using data from the PEPSI, HARPS-N, and TRES spectrographs across multiple epochs, we apply least-squares deconvolution to measure out-of-transit stellar radial velocities. With the PEPSI and HARPS-N data sets, we measure in-transit planet radial velocities using transmission spectroscopy. By fitting the circular orbital solution that captures these Keplerian motions, we recover a planetary dynamical mass of 2.17 ± 0.56 M J and stellar dynamical mass of 2.11 ± 0.78 M ⊙, both of which agree with the discovery paper. Furthermore, we argue that this system, as well as systems like it, are highly overconstrained, providing multiple independent avenues for empirically cross-validating model-independent solutions to the system parameters. We also discuss the implications of this revised mass for studies of atmospheric escape.

Passenger Capacity and Safety: Investigating the Likelihood of COVID-19 Transmission in Public Transport Modes within Makurdi Metropolis

Following its outbreak in the Wuhan region of China, the spread of Corona Virus (COVID-19) across the world has threatened national and local authorities or policy makers and transport experts due to its effects on human mobility. This study investigated the transmissibility of COVID-19 among passengers in transit using public transport modes in Makurdi metropolis. Analytical simulation using stochastic search method called Genetic Algorithm (GA) technique was used to simulate the transmissibility of the infectious disease within enclosed spaces of transport modes based on layouts and capacities. The sum of arithmetic sequence was used as the objective function of individual arrivals in each mode, it was minimised to obtain optimum safe capacity. Capacities of public transport modes were subdivided into; Normal, Above Normal and Below Normal (50% of Normal). Findings of the experiments indicated that optimum safe capacity of minibuses, taxies, tricycles and motorcycles used in Makurdi metropolis were at 8, 3, 2 and 1 person respectively. This occurred at 50% capacity which agreed with findings of previous studies. An efficient and sustainable public transport policy framework was designed for policy makers and transport experts to help achieve safe travel and healthier living in Makurdi metropolis during the COVID-19 era. Keywords— COVID-19 transmission, Makurdi Metropolis, Modal Split, Mode capacity, Passenger Safety, Public Transport.