Identification of Novel Micropeptides Derived from Hepatocellular Carcinoma-Specific Long Noncoding RNA

Identification of cancer-specific target molecules and biomarkers may be useful in the development of novel treatment and immunotherapeutic strategies. We have recently demonstrated that the expression of long noncoding (lnc) RNAs can be cancer-type specific due to abnormal chromatin remodeling and alternative splicing. Furthermore, we identified and determined that the functional small protein C20orf204-189AA encoded by long intergenic noncoding RNA Linc00176 that is expressed predominantly in hepatocellular carcinoma (HCC), enhances transcription of ribosomal RNAs and supports growth of HCC. In this study we combined RNA-sequencing and polysome profiling to identify novel micropeptides that originate from HCC-specific lncRNAs. We identified nine lncRNAs that are expressed exclusively in HCC cells but not in the liver or other normal tissues. Here, DNase-sequencing data revealed that the altered chromatin structure plays a key role in the HCC-specific expression of lncRNAs. Three out of nine HCC-specific lncRNAs contain at least one open reading frame (ORF) longer than 50 amino acid (aa) and enriched in the polysome fraction, suggesting that they are translated. We generated a peptide specific antibody to characterize one candidate, NONHSAT013026.2/Linc013026. We show that Linc013026 encodes a 68 amino acid micropeptide that is mainly localized at the perinuclear region. Linc013026-68AA is expressed in a subset of HCC cells and plays a role in cell proliferation, suggesting that Linc013026-68AA may be used as a HCC-specific target molecule. Our finding also sheds light on the role of the previously ignored ’dark proteome’, that originates from noncoding regions in the maintenance of cancer.

LOCALIZATION: A TRANSLATION STRATEGY OR A SEPARATE TOOL?

The article discusses different approaches to localization – as one of translation strategies and as a separate tool for rendering language messages in another language. The study is done with the help of comparative linguistic analysis of original movie titles in the English language and their official Russian versions. We study ways of rendering of the film titles in the target language and reveal some reasons for localization. In conclusion, we provide a list of motives for language localization of the movie titles: 1) tendency to build the name of a new film into the system of films already known to the Russian viewer or other cultural realities with the help of socalled "references" to familiar nominations; 2) give additional advertising to the film, 3) make the film more attractive to a specific target audience.

Referring to arbitrary entities with placeholders

A speaker/writer uses a placeholder (PH) to fill in the syntactic slot of a target word when she has no immediate access to the word or prefers to avoid explicitly mentioning it for contextual reasons. In the present article, I point out a hitherto understudied usage of PHs: a speaker/writer who does not have in mind a specific target form may use a PH to refer to an arbitrary entity (e.g. person, object, action, event, proposition). I substantiate this claim by analysing a variety of original data on Japanese wh-derived PHs. Further evidence for this claim comes from a cross-linguistic survey of wh-derived PHs in Korean and demonstrative-derived PHs in Romanian and Bulgarian. I show that the arbitrary-referential function is observed in PHs in all these languages, regardless of their origins (i.e. wh word, demonstrative) and their categories (i.e. nominal, verbal).

Pengembangan dan Sosialisasi Aplikasi E-Commers Parea di KUBE Sekar Muria Hadipolo Kudus

Hadipolo Village is one of the villages in Jekulo District, Kudus Regency. In this village there are several bitter melon farmers who process it into snacks that are nutritious and very beneficial for health. So far, the marketing process for bitter melon chips is still done traditionally so that sales of bitter gourd chips are not optimal in quantity and the range of promotions is still limited around the city of Kudus, although there are some requests from outside the city but the numbers are still large. still a little because of lack of promotion. The purpose of this community service is to develop and socialize e-commerce applications which are expected to help and increase the income and welfare of the bitter melon chip craftsmen in Hadipolo Village. With a specific target in the use of Pare e-commerce, namely increasing the marketing quantity of bitter gourd chips by expanding the marketing area by promoting the results of bitter melon chips through e-commerce so that they can reach a wider market. Pare e-commerce is an e-commerce used by Kube Pare chips in developing and marketing products from Pare chips. The way this system works is by conducting online buying and selling transactions, so that the marketing area is also wider. Abstrak Desa Hadipolo merupakan salah satu desa yang berada di Kecamatan Jekulo Kabupaten Kudus. Di desa ini terdapat beberapa petani pare yang mengolahnya menjadi makanan ringan yang berkhasiat dan sangat bermanfaat bagi kesehatan. Selama ini proses pemasaran keripik pare masih dilakukan secara tradisional sehingga penjualan keripik pare secara kuantitas belum maksimal dan jangkauan promosi masih terbatas di sekitar kota Kudus, walaupun ada beberapa permintaan dari luar kota namun jumlahnya masih banyak. masih sedikit karena kurang promosi. Tujuan dari pengabdian masyarakat ini adalah untuk pengembangan dan sosialisasi aplikasi e-commerce yang diharapkan dapat membantu dan meningkatkan pendapatan, kesejahteraan para pengrajin keripik pare di Desa Hadipolo. Dengan target khusus dalam penggunaan e-commerce Parea yaitu meningkatkan kuantitas pemasaran keripik pare dengan memperluas wilayah pemasaran dengan mempromosikan hasil keripik pare melalui e-commerce sehingga dapat menjangkau pasar yang lebih luas. E-commerce Parea merupakan e-commerce yang digunakan Kube keripik pare dalam mengembangkan dan memasarkan produk dari keripik Pare tersebut. Cara kerjanya dari sistem tersebut adalah dengan melakukan transaksi jual beli secara online, agar wilayah pemasaran juga semakin luas.

Immunological Separation of Bioactive Natural Compounds from Crude Drug Extract and Its Application for Cell-Based Studies

In this study, we present a review on a useful approach, namely, immunoaffinity column coupled with monoclonal antibodies (MAbs), to separate natural compounds and its application for cell-based studies. The immunoaffinity column aids in separating the specific target compound from the crude extract. The column capacity was stable even after more than 10 purification cycles of use under the same conditions. After applying the crude extract to the column, the column was washed with washing buffer and eluted with elution buffer. The elution fraction contained the target compound bound to MAb, whereas the washing fraction was the crude extract, which contained all compounds except a group of target compounds; therefore, the washing fraction was referred to as a knockout (KO) crude extract. Cell-based studies using the KO extract revealed the actual effects of the natural compounds in the crude extract. One-step separation of natural compounds using the immunoaffinity column coupled with MAbs may help in determining the potential functions of natural compounds in crude extracts.

Pelatihan Pembuatan Desinfektan Yang Aman Dan Ramah Lingkungan Di Wilayah Awipari Sebagai Upaya Pencegahan Penyebaran Covid-19

This community service activity is motivated by the potential for the spread of the Covid virus which is still happening and the Awipari Village does not yet have health facilities that are balanced with its population. In addition, the use of disinfectants as a protection for families and the environment from the spread of the Covid-19 virus is still not available with natural ingredients and is safe for the environment. This is what training on the manufacture of safe and environmentally friendly disinfectants needs to be done. The main purpose of this community service is to help target partners to be more independent in providing the disinfectant needs that residents need. Furthermore, it can be used as an alternative field to increase household income. The specific target to be achieved in the future is that target partners are able and accustomed to using safe and environmentally friendly basic materials in making disinfectants. Community service activities will be held in September 2021 at the Awipari Village Office, Tasikmalaya City. The methods used are lectures, discussions, and the practice of making disinfectants directly. Community service activities are very well received by partner community groups. The community is enthusiastic and active in demonstrations and discussions and wants this service activity to be routine and continue with various training themes according to community needs.

PENYUSUNAN LAPORAN ARUS KAS DENGAN METODE LANGSUNG

The problem faced by partner, Lembaga Beasiswa Dharma Pembangunan, is that the students have not received material on the preparation the statement of cash flows in school. The team from Faculty of Economic and Business majoring in Accounting, Tarumanagara University, offer a solution by providing training on the preparation the statement of cash flows. The specific target to be achieved from this training is to increase the knowledge of the students at Lembaga Beasiswa Dharma Pembangunan, especially regarding to statement of cash flows. The material that will be provided is expected to provide benefits to these students when they work or continue their education to a higher level, especially those related to the accounting. There are several stage carried out in yhe implementation of this activities at Lembaga Beasiswa Dharma Pembangunan. First, a survey was conducted to find out what material is needed by the partner. Based on this survey, it is known that the material needed by parter is about preparation the statement of cash flows. Furthermore, the team will prepare the material that will given to the student and provide online training due to the pandemic caused by covid 19 virus. In the last stage, the team will make mandatory and additional outputs, and prepare the final and financial reports. Overall, this activity was carried out from July to December 2021.Permasalahan yang dihadapi oleh pihak mitra yaitu Lembaga Beasiswa Dharma Pembangunan adalah para siswa siswi yang ada di lembaga tersebut belum mendapatkan materi mengenai penyusunan laporan arus kas di sekolah. Tim dosen dan mahasiswa Fakultas Ekonomi dan Bisnis jurusan Akuntansi Universitas Tarumanagara, menawarkan solusi dengan memberikan pelatihan mengenai penyusunan laporan arus kas. Tujuan maupun target khusus yang hendak dicapai dari adanya pelatihan ini adalah agar pengetahuan dari para siswa siswi yang ada di Lembaga Beasiswa Dharma Pembangunan dapat bertambah, khususnya mengenai laporan arus kas. Materi yang akan diberikan ini diharapkan dapat memberikan manfaat kepada siswa siswi ini pada saat mereka nanti bekerja atau melanjutkan pendidikan ke tingkat yang lebih tinggi, terutama yang berkaitan dengan bidang akuntansi. Terdapat beberapa tahap yang dilakukan dalam pelaksanaan kegiatan PKM di Lembaga Beasiswa Dharma Pembangunan ini. Pertama yaitu dilakukan survei untuk dapat mengetahui materi apa yang dibutuhkan oleh pihak mitra. Berdasarkan dari survei tersebut maka diketahui bahwa materi yang dibutuhkan oleh pihak mitra untuk dilakukan pelatihan adalah materi mengenai penyusunan laporan arus kas. Selanjutnya tim dosen dan mahasiswa akan mempersiapkan materi berupa teori maupun contoh soal serta penjelasannya yang akan diberikan kepada para siswa siswi serta memberikan pelatihan secara online yang dikarenakan adanya pandemi yang disebabkan oleh virus covid 19. Pada tahap terakhir, kegiatan ini akan diakhiri dengan membuat artikel SERINA dan PINTAR sebagai luaran wajib dan luaran tambahan, serta menyusun laporan akhir dan laporan keuangan atas kegiatan yang telah dilakukan. Secara keseluruhan kegiatan PKM ini dilakukan sejak bulan Juli sampai dengan bulan Desember 2021.

Resource Allocation Among Competing Innovators

Many innovative products are designed to satisfy the demand of specific target consumers; thus, the innovators will inevitably compete with each other in the product market. We investigate how a profit-maximizing principal should properly allocate her limited resources to support the innovations of multiple potentially competing innovators. We find that, as the available resources increase, the optimal diversification of investment may first increase and then decrease. This interesting nonmonotone pattern is driven by a trade-off between the risk of innovation failure and rent dissipation because of competition. Using this framework, we also analyze a nonprofit principal seeking to maximize the total number of successful innovations, the probability of at least one innovator succeeding, consumer surplus, and total social welfare. A nonprofit principal tends to invest more diversely compared with a for-profit counterpart. This paper was accepted by Sridhar Tayur, entrepreneurship and innovation.