The Effectiveness Of Maturation Stimulation Method On Fecundity And Egg Diameter Of Freshwater Lobster (Cherax quadricarinatus)

Losbter air tawar (LAT) merupakan komoditas perikanan yang memiliki banyak keunggulan sehingga oleh Kementerian Kelautan dan Perikanan LAT dijadikan komoditas budidaya andalan. Akibatnya permintaan benih LAT semakin meningkat, sementara secara alami LAT hanya memijah 2 kali per tahun. Salah satu upaya untuk meningkatkan performa reproduksi LAT yaitu pemilihan metode perangsangan maturasi yang tepat. Sehingga perlu diteliti tentang beberapa metode perangsangan maturasi induk LAT yang dapat meningkatkan performa reproduksi yaitu fekunditas dan diameter telur LAT. Rancangan yang digunakan dalam penelitian ini adalah rancangan acak lengkap, dengan 4 perlakuan dan 3 kali pengulangan. Adapun perlakuan dalam penelitian ini adalah P1 (kontrol), P2 (ablasi tangkai mata), P3 (penambahan vitamin E pada pakan), dan P4 (penyuntikan hormon). Berdasarkan hasil penelitian, perlakuan metode perangsangan maturasi yang berbeda memberikan pengaruh yang tidak berbeda nyata (P>0.05) terhadap fekunditas dan diameter telur, namun berbeda nyata dengan perlakuan kontrol. Sehingga dapat disimpulkan bahwa metode maturasi yang berbeda hanya dapat mempercepat maturasi pada induk LAT dibanding perlakuan kontrol.

Effects of GnRH and Anti-Dopamine on Gonad Maturation of Osteochilus melanopleurus (Bleeker, 1852)

The Osteochilus melanopleurus (Bleeker, 1852) is one of the endogenous fish in the Siak River waters of Riau province which has high economic value. However, the fulfillment of needs still depends on the catch in nature. Efforts to overcome these problems are the availability of mature gonads. Broadly to accelerate the maturation of gonads through hormone induction until the fish are ready to be spawned to produce fish fry continuously. The purpose of this study was to analyze the effect of gonadotropin and anti-dopamine hormone induction with the trademark “Oodev” on the final gonad maturity (TKG) of the prospective broodstock of O. melanopleurus. The research was conducted from April to August 2021 in the fish rearing column and the experimental pond, Faculty of Fisheries and Marine Science. The research method was a Completely Randomized Design with 4 treatment levels and 3 replications, while the treatments were P0 (without Oodev injection), P1 (Oodev dose 0.5 mL/kg), P2 (0.7 mL/kg), and P3 (0,9 mL/kg). Measured responses: percentage of broodstock that reached TKG and egg diameter. The results of the research that have been carried out, the measurement of the diameter of the eggs produced showed that the use of the Oodev hormone at a dose of 0.7 mL/kg body weight of O.melanopleurus given every week for twelve weeks was able to stimulate the development of the gonads of O.melanopleurus. Ovaprim injection of 0.7 mL/kg body weight produced 89,566 eggs with a latency of 4 hours. oodev injection dose of 0.7 ml/kg BW or P2 treatment has succeeded in achieving a TKG of 82%.

A Study on Some Biological Parameters of Crayfish (Astacus leptodactylus Eschscholtz, 1823) in Ulugöl, Samsun, Turkey

The study aimed to investigate some morphometric traits, length-weight relationship, meat yield, fecundity and some biochemical compositions of Astacus leptodactylus, in one of the Bafra Fish Lakes named Ulugöl, Samsun, Turkey. A total of 378 A. leptodactylus (198 female and 180 male) were examined. The average carapace length was 50.96±0.46 mm for females, 51.31±0.66 mm for males and 51.13±0.40 mm for all sampled crayfish. Abdomen length was 52.36±0.49 mm for females, 49.26±0.62 mm for males and 50.87±0.40 mm for all sampled crayfish. Live weight was between 14.38 g and 105.03 g with an average of 38.26±0.73 g. The average weight of females was less than that of males. There was a strong relationship between length and weight. The weight of crayfish with pleopodal eggs ranged from 28.24 g, to 59.50 g with an average of 41.73±1.09g. The average number of eggs per individuals was 192.90±9.94 with an average number of 4.62±0.21 per unit of live weight. The average total egg weight was 3.35±0.19 g while the average egg diameter was 2.17±0.03 mm. Abdomen meat was lower for males comparing with females. Average moisture, crude ash, crude oil and crude protein of abdomen meat were 81.27%, 1.47%, 0.81% and 16.45%, respectively.

Perkembangan Awal Larva Ikan Mas (Cyprinus carpio L) dari Induk yang Diberi Pakan dengan Penambahan Kurkumin

Perkembangan awal larva sangat dipengaruhi oleh ketersediaan makanan yang berasal dari kantung telurnya. Tidak terpenuhi nutrisi pada masa awal perkembangan dapat mempengaruhi perkembangan larva. Tujuan penelitian ini adalah untuk mengukur diameter telur hasil pemijahan dan panjang badan pada awal perkembangan larva ikan mas dari induk yang diberi pakan dengan penambahan kurkumin. Penelitian ini menggunakan induk betina ikan mas yang diberikan suplementasi kurkumin pada beberapa dosis yaitu 0%; 0,25%; 0,5%; 1%; dan 2%. Ikan yang telah matang gonad kemudian dipijahkan. Telur yang telah terbuahi kemudian diambil untuk ditetaskan dan diamati perkembangan larvanya selama 2 hari setelah penetasan (hsp). Hasil pengamatan menunjukkan bahwa perlakuan suplementasi kurkumin dengan dosis 0,5% yang diberikan dalam pakan induk betina ikan mas memberikan nilai yang optimal terhadap diameter telur hasil pemijahan (p<0,05) dan panjang badan larva sampai pada umur 2 hsp (p<0,05), namun panjang mutlak larva sampai pada 2 hsp tidak menunjukkan adanya perbedaan antar perlakuan (p>0,5). Berdasarkan hasil penelitian ini dapat disimpulkan bahwa suplementasi kurkumin dengan konsentrasi 0,5% pada induk betina ikan mas dapat mengoptimalkan pertumbuhan larva pada awal kehidupannya.Kata kunci : Cyprinus carpio, kurkumin, larva Early Development of Common Carp Larva from Broodstock Fed Diet Supplemented with CurcuminABSTRACTThe early growth of the larva is greatly influenced by the availability of food from the egg yolk. Insufficiency of nutrition in the early stages of development can affect the development of larva. The purpose of this study was to know the early growth of larva from broodstock supplemented with curcumin. This study used the female broodstock of common carp that supplemented with curcumin at several doses; 0%; 0,25%; 0,5%; 1%; and 2%. The fishes that had been mature were spawned, and the fertilized eggs were taken to be hatched. The larva was observed for 2 days after hatching (dah). The results showed that the treatment of curcumin supplementation with a dose of 0,5% given in the common carp feed gave an optimal value for egg diameter (p <0,05) and body length of the larva until the age of 2 dah (p <0,05), but did not differ for absolute length growth (p>0,05).Key words: Cyprinus carpio, curcumin, larva

Physical quality of eggs of four strains of poultry

The layer poultry is an important activity for food production with high biological value. Measuring egg quality has great relevance to ensure safety and quality products for consumers. Thus, the objective was to evaluate the egg physical quality of four laying hen’s strains. Were used 864 eggs from four laying hens’ lines (Hisex Brown®, Hy-Line Brown®, Isa Label®, and Lohmann Brown®). The experimental design was completely randomized composed of four treatments (strains) with nine replications with four eggs each. Egg weight, egg diameter, egg length, specific gravity, yolk, albumen and shell weight and percentage, Haugh units, and shell thickness were evaluated. There was a significant difference for all parameters evaluated. The Hisex Brown® strain showed the best results for egg diameter, egg length, specific weight, albumen height, Haugh units, yolk weight, albumen%, shell weight, shell%, and shell thickness, while the Hy-Line Brown® produced bigger and heavier eggs, and Isa Label® presented the highest yolk%. The Hisex Brown® strain showed the better physical quality of eggs when compared to the other studied lines, the strain being indicated when the objective is to produce eggs with better internal and external quality.

Short Communication: Investigating gonad length and fecundity in mudskippers (Gobiidae) at the Musi River estuary, South Sumatra, Indonesia

Ridho MR, Patriono E, Wati DR, Pratama R, Avesena M. 2021. Short Communication: Investigating gonad length and fecundity in mudskippers (Gobiidae) at the Musi River estuary, South Sumatra, Indonesia. Biodiversitas 22: 4413-4420. Information on the biological aspects of mudskipper (Gobiidae) reproduction is needed as a conservation effort to use economically valuable and sustainable resources in the future. This can be used to enrich the information on the status of the mudskipper research at the Musi River estuary, South Sumatra, Indonesia. The field sampling was carried out by using the purposive sampling method. The sampling of fish was carried out by the researchers and assisted by the people of the community using fishing rods and bubu fish traps. The results showed that 122 mudskipper were obtained, which consisted of four species Boleophthalmus boddarti, Boleophthalmus dussumieri, Periophthalmus chrysospilos, Periophthalmodon schlosseri. 93 male and 29 female fish were caught, the sex ratio was 3.20:1. There are more male than female fish overall with the sampling method used. Most of the female fish fecundity was reported in P. chrysospilos, namely 23,270-36,264 items in GM (III) and ranged from 40,825-87,749 items in GM (IV). The largest egg diameter was reported in P. chrysospilos by 0.051-0.069 mm (GML III) and 0.053-0.062 mm (GML IV), while the smallest weight and length were reported in P. chrysospilos than other species. The egg size was not positively correlated with body size.

The development of the reproductive organs of the hybrid grouper cantik (Epinephelus fuscoguttatus x E. polyphekadion)

Observation of the reproductive organs of the hybrid grouper cantik was carried out. Cantik grouper is the result of cross-breeding between female tiger grouper Epinephelus fuscoguttatus and male camouflage grouper E. polyphekadion. This study aimed to determine the development of the reproductive organs of cantik grouper. The observed test fishes were 3 fishes of Cantik grouper with a total length of 42 - 49 cm and a bodyweight of 1.45 - 2.26 kg. The parameters observed were the concentarion of estradiol and testosterone in the blood using the ELISA kit, gonad development, and histological maturity level of the gonads. The results showed that the hormone testosterone found in the sample cantik no 1 was 9353 pg/ml, while the hormone estradiol was found in all samples of beautiful grouper in the range of 291 - 1133 pg/ml. From the dissected fish, the gonads had developed and weighed 3.12 - 56.01 g and one sample had a fecundity of 265,800 eggs with an egg diameter of 360-520 micron. Gonadal histology shows oosit development at maturity levels I, II, and IV. This indicates that the crossbreeding between female tiger grouper and male camouflage grouper produces hybrid grouper cantik that are fertile, which is shown by their reproductive organs to have developed well.

Gonad development and blood plasma profile of female bada fish (Rasbora sp.) by giving a combination of the hormones estradiol and spirulina in the feed

This study aimed to evaluate the effect of the combination of estradiol-17β hormone and spirulina in feed on blood plasma biochemistry, histology, and gonad development of female bada fish (Rasbora argyrotaenia). The research method were an experimental study using a completely randomized design with four treatment combinations and four replications, namely without the addition of the estradiol-17β hormone and spirulina (P0), the addition of the estradiol-17β hormone at 7 g kg-1 feed (P1), the addition of spirulina at 30 g kg-1 feed (P2) and the addition of the estradiol-17β hormone at 7 g kg-1 feed and spirulina at 30 g kg-1 feed. Female bada fish (4.22±1.28 g) were kept in an aquarium measuring 100x50x50 cm3 with a density of seven individuals per aquarium. Fish were fed the treatment twice daily, with 3% of the biomass for six weeks. The test parameters were estradiol-17β hormone concentration, egg diameter, blood plasma glucose concentration, blood plasma total cholesterol concentration, and gonadal histology observations. The experimental results showed that the concentration of the estradiol-17β hormone in the body plasma of treatments P1 and P3 was statistically significantly different (P<0.05) compared to treatments P0 and P2. The combination of estradiol-17β hormone and spirulina added to the feed gave not significantly different results (P>0.05) on blood plasma biochemistry and egg diameter of bada fish broodstock. A combination of estradiol-17β hormone and spirulina hormones in the feed resulted in better P1 and P3 treatments with higher plasma concentrations of the estradiol-17β hormone. Observations of gonadal development from histology showed that the bada fish gonads belonged to the asynchronous type.

Reproductive Characteristics of the Pond-Farmed Sultan Fish (Leptobarbus hoevenii)

Highlight ResearchThe reproductive characteristics of L. hoevenii were examinedThe potentially smallest maturity sizes of female and male L. hoevenii were 350 g and 180 g, respectivelyThe fecundity of a 1,000 g L. hoevenii can be more than 100,000 eggsL. hoevenii was determined as a multiple spawnerAbstractSultan fish (Leptobarbus hoevenii) is a high value freshwater fish, cultured in some Southeast Asian countries, including Malaysia and Thailand. However, information on its reproductive characteristics is very scarce. This study examined the gonadosomatic index (GSI), fecundity, egg diameter, and determined whether L. hoevenii is a single- or multiple-spawner. Twenty male and female pond-farmed L. hoevenii broodstock were obtained to measure their total length (TL), body weight (BW) and gonad weight to calculate the GSI. Ten females were randomly sampled from the 20 to determine their fecundity. A total of 1,500 eggs were sampled from each female. The egg diameter was measured then its frequency distribution was analyzed to detect the number of egg class group, and to determine whether L. hoevenii is a single- or multiple spawner. The female L. hoevenii examined were 32.2-47.1 cm and 350-1,200 g, while the males were 30.7-45.8 cm and 180-970 g in TL and BW, respectively. All female specimens contained gonads. The potentially smallest mature samples were recorded at 350 g (female) and 180 g (male). GSI for the female and male L. hoevenii were 1.81-12.28 % and 1.03-5.09 %, respectively. The fecundity was 35,467 -128,067 eggs, while the highest fecundity was observed in a 1,000 g fish. The observed egg diameter ranged from 500 to 1,855 μm. Two to five groups of egg class were detected, indicating that L. hoevenii is a multiple spawner.

Factors affecting Oithona nana and Oithona similis along a salinity gradient

Spatial and temporal variations of reproduction variables of the two cyclopoid copepods Oithona nana Giesbrecht, 1893 and Oithona similis Claus, 1866 were evaluated monthly from September 2007 to August 2008 in four ponds at different salinities in the solar saltern of Sfax, Tunisia, under hypersaline conditions. Along the salinity gradient the abundance of O. nana and O. similis declined from 14,900 ± 15,200 ind. m-3 (A1) to 6,800 ± 7,200 (C31) and from 13,800 ± 17,100 ind. m-3 (A1) to 3,700 ± 5,800 (C31), respectively. The findings showed that the abundance of these Oithona species, particularly the ovigerous females, was positively correlated with the abundance of Diatomeae, and negatively correlated with salinity. The egg production rate (EPR), the egg diameter, the clutch size, as well as the cephalothorax length of mature females, were all negatively correlated with salinity and positively correlated with the abundance of Diatomeae. Along the existing salinity gradient, the EPR decreased from 4.7 ± 0.39 (A1) to 3.2 ± 0.13 (C31) eggs female-1 day-1 and from 5.19 ± 0.46 (A1) to 3.66 ± 0.05 (C31) eggs female-1 day-1 for O. nana and O. similis, respectively, and this could mainly be attributed to the hypersaline conditions. The positive relationship between the abundance of Diatomeae with all traits shows, that these may be a vital component of the diet for the investigated species. The reduction of body size of Oithona along the salinity gradient demonstrates that such a character could mainly be due to a network of causes, related with the confinement and not only to salinity.