defatted soybean meal
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LWT ◽  
2022 ◽  
Vol 156 ◽  
pp. 113034
Author(s):  
Yujie Zhang ◽  
Yixu Feng ◽  
Huiqin Shi ◽  
Kaili Ding ◽  
Xinyun Zhou ◽  
...  

2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Muhamad Aidilfitri Mohamad Roslan ◽  
Zulfazli M. Sobri ◽  
Ali Tan Kee Zuan ◽  
Sim Choon Cheak ◽  
Nor Aini Abdul Rahman

AbstractThe extraction of soluble hydrolysate protein and sugar from a biomass cocktail of defatted soybean meal (DSM) and jackfruit peel (JP) was examined using microwave-alkaline hydrolysis by varying the NaOH concentrations (0.04–0.11 M) and residence times (2–11 min). Based on the central composite design, the optimized parameters were achieved at 0.084 M NaOH concentration (100 mL), for 8.7 min at 300 W microwave power level to obtain the highest protein (5.31 mg/mL) and sugar concentrations (8.07 mg/mL) with > 75% recovery. Both raw and detoxified hydrolysate (using activated carbon) were correspondingly biocompatible with Enterobacter hormaechei strain 40a (P > 0.05) resulting in maximal cell counts of > 10 log CFU/mL. The optimized hydrolysate was prepared as an additive in molasses-alginate bead encapsulation of strain 40a. Further evaluation on phosphate and potassium solubilization performance of the encapsulated strain 40a exhibited comparable results with those of free cell counterpart (P > 0.05). The DSM-JP hydrolysate cocktail holds potential as a carrier additive of encapsulated-cell bead biofertilizers in order to sustain bacterial cell quality and consequently improve crop growth and productivity.


Agronomy ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 996
Author(s):  
Muhamad Aidilfitri Mohamad Roslan ◽  
Izzalan Sohedein ◽  
Puan Sheau Ling ◽  
Zulfazli M. Sobri ◽  
Ali Tan Kee Zuan ◽  
...  

The application of plant beneficial bioinoculants such as phosphate solubilizing bacteria is a sustainable approach to expanding crop performance in agriculture. However, bioinoculant strains, particularly non-sporulating bacteria are often exposed to detrimental conditions throughout the production process and a long period of storage. This will negatively influence their viable cell density and eventually limit its efficacy in the field. To overcome such a scenario, an optimal formulation of biofertilizer should be prioritized. In this report, a sustainable valorization of molasses and defatted soybean meal as formulation of biofertilizer enriched with Enterobacter hormaechei 40a was proposed. Through the two-level factorial design and central composite design, the optimal formulation and fermentation conditions of bio-organic fertilizer to achieve maximum cell density of strain 40a were achieved. The highest cell density of strain 40a in the optimized molasses-DSM (OMD) medium was 12.56 log CFU/mL after 24 h which was 99.7% accuracy towards the predicted value. Interestingly, the solubilized P was increased by 62.4% in the OMD medium (174.07 µg/mL P) as compared to the standard P medium (65.38 µg/mL P). The shelf life of strain 40a after 180 days of storage was improved significantly around 10 log CFU/mL, when the OMD medium was amended with 0.1% sodium alginate. The strategy described here offers opportunities for agronomic formulation and large-scale bio-organic fertilizer production in the agriculture industry.


BioResources ◽  
2020 ◽  
Vol 15 (3) ◽  
pp. 6385-6403
Author(s):  
Jiaheng Liu ◽  
Xiangyu He ◽  
Yuhui Du ◽  
Itsanun Wiwatanaratanabutr ◽  
Guangrong Zhao ◽  
...  

This work aimed to study the potential of defatted rice bran (DRB) and defatted soybean meal (DSM) as carbon and nitrogen sources for Lactococcus lactis growth and nisin production. First, a maximum nisin yield of 3630 IU/mL was achieved using 40% DRB hydrolysates and 30% DSM hydrolysates, which was 1.13 times greater than that found in commercial media. Second, to simplify the operation and shorten the length of the entire process, the processes of combined hydrolysis of DRB-DSM followed by fermentation, and simultaneous hydrolysis and fermentation of DRB-DSM were developed. Neutral proteinase enhanced the saccharification of DRB by cellulase and α-amylase. Furthermore, the strategy of NADH oxidase expression and hemin addition was innovatively proposed to overcome the oxygen stress in a simultaneous hydrolysis and fermentation process, which could alleviate the lag period following inoculation of L. lactis and result in a 77.3% increase in nisin titer.


2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Kadry Z. Ghanem ◽  
Mohamed Z. Mahran ◽  
Manal M. Ramadan ◽  
Hassan Z. Ghanem ◽  
Mohamed Fadel ◽  
...  

Author(s):  
Paula Fernandes de Siqueira Machado ◽  
Carlos Francisco Pedroso ◽  
Roberto de Campos Giordano ◽  
Paulo Waldir Tardioli ◽  
Raquel de Lima Camargo Giordano

LWT ◽  
2018 ◽  
Vol 97 ◽  
pp. 496-502 ◽  
Author(s):  
Yaqi Zhao ◽  
Dongxiao Sun-Waterhouse ◽  
Mouming Zhao ◽  
Qiangzhong Zhao ◽  
Chaoying Qiu ◽  
...  

2018 ◽  
Vol 58 (4) ◽  
pp. 689 ◽  
Author(s):  
R. Manning

Artificial pollen substitutes were developed to improve productivity from honeybees during periods of nutrient scarcity. The history of pollen-substitute development is outlined. Although many attempts have been made, no substitute has the same nutritional value as bee-collected pollen. Following a review of honeybee nutrition, Black (2006) described the ingredient and nutrient specifications for a pollen substitute, including the need for attractiveness to honeybees. Protein isolates were recommended to avoid toxicity from carbohydrates found in many ingredients used in previous studies. Twenty-seven plant- and animal-derived oils and a rum supplement, mixed at 2% with a low-lipid irradiated pollen, were evaluated for attractiveness by measuring consumption and bee congregation when placed in dishes within beehives. Coconut, linseed oil and rum were preferred significantly (P < 0.05) to the pollen, whereas pollen was preferred to lavender and sage oils. Almond and evening primrose oils were also highly, but not significantly, preferred compared with pollen and were used in combinations with coconut and linseed oils in subsequent experiments. Eleven predominantly pure protein sources, either singly or in combination, were mixed with 2% or 5% attractive oils and evaluated for attractiveness. Soybean protein isolate was selected, because attractiveness was not significantly different from bee-collected pollen. It was then used with oils in an experiment to evaluate either powdered cellulose or milled oat hulls as a fibre source. There were no significant differences in attractiveness of substitutes with the two fibre sources or pollen, and powdered cellulose was selected for further use on the basis of availability. An experiment with ~1000 newly hatched bees with a fertile queen in cages was undertaken to evaluate the diets when given as the sole nutrient source. Consumption, bee longevity and estimated hypopharyngeal gland development using head weight were measured One artificial diet (PI-5) contained 30% soybean protein isolate, 10% cellulose, 42% icing sugar, 12.5% water, 4.5% mixed oils and 1.3% minerals and vitamin plus cholesterol. Diet (PI-10) was similar, but contained twice as much oil replacing icing sugar. Other treatments were redgum pollen (P), a commercial pollen substitute, Feedbee® and defatted soybean meal. Diet consumption, lifespan and head weights were significantly less for the PI diets than for P, while Feedbee® and defatted soybean-meal diets were generally intermediate. The PI diets were discovered to contain excess sodium, due to the manufacturing process. Bees consuming the PI diets had lower concentrations of magnesium, copper, iron, manganese and zinc in their bodies than did those offered P. The fatty acid content of bee bodies also varied with diet. A revised formulation is recommended with reduced sodium and modified mineral and fatty acid composition.


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