Phytoconstituents of Lantana camara L.: Rekindling Hope in the Cancer Treatment

Background: Lantana camara L. belongs to the family Verbenaceae. It originated in Tropical America in Southern Georgia and to the North of Texas and was introduced in Calcutta, India in the year 1809 as an ornamental hedge. The plant L. Camara is also distributed in Southeast Asia, China, Australia, Brazil, West Indies, Kenya, Mexico, East Africa, Tanzania. Many of its phytoconstituents possess medicinal properties which are used traditionally to treat fever, uterine hemorrhage, and excess menstrual discharge, chronic ulcers, rheumatism, gonorrhea, toothache, gastrointestinal pain, etc, and has been used in Brazil for curing malaria, mange, headaches, colds, and fevers. Objectives: The review elaborates traditional practices, phytochemistry of Lantana camara L. along with the role of Lantana camara in various types of cancers. Method: The data on L. camara was collected through different online databases like Web of Science, PubMed, Science Direct, Springer, and Google Scholar. Results: Major phytoconstituents isolated from the plant shows anticancer activity specially lantadene A-D, icterogenin, oleanolic acid, lantacamaric acid A, B, oleanonic acid, etc. In vitro and in vivo studies demonstrate its potential for various cancers. Certain extracts, isolated compounds, and their semi-synthetic derivatives have depicted a significant cytotoxic and anti-proliferative effect. Conclusion: Clinical studies are not yet established, therefore, making it crucial to direct future researches in that area.

Genotoxic and Cytotoxic Activities of Lantadene A-Loaded Gold Nanoparticles (LA-AuNPS) in MCF-7 Cell Line: An in vitro Assessment

Gold nanoparticles (AuNPs) have been widely used in many applications. Their usage as drug delivery vehicles has also gained considerable attention due to their chemical and optical properties as well as their good biocompatibility. The present study was conducted to evaluate the efficiency of AuNPs in enhancing the cytotoxic and apoptotic induction activity of lantadene A (LA), separated from Lantana camara leaves, on the breast tumor cell line MCF-7 in vitro. By utilizing plant-mediated synthesis method of nanostructures, LA-loaded AuNPs (LA-AuNPs) were prepared and their formation was confirmed by means of ultraviolet–visible spectroscope, atomic force microscope, scanning electron microscope, and zeta potential. The cytotoxic effect of LA-AuNPs was analyzed using a methylthiazol tetrazolium assay and compared to free AuNPs and LA. The results indicated a significant increase in the reduction of MCF-7 cells viability after incubation with LA-AuNPs. As determined by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay, LA-AuNPs induced a greater ratio of DNA-fragmented cells compared to LA-treated and untreated cells. Also, by operating real-time polymerase chain reaction, LA-AuNPs-treated cells displayed an increased upregulation of p53 expression and downregulation of BCL-2 expression in addition to a significant reduction in the level of BCL-2-BAX ratio. No significant effect was shown on the expression of BAX. Collectively, our results indicate that LA-AuNPs showed promising cytotoxicity to MCF-7 cells as a novel nanoscale preparation, likely via induction of apoptotic genes and stimulation of DNA fragmentation.

Herbicidal bioactivity of natural compounds from Lantana camara on the germination and seedling growth of Bidens pilosa

ABSTRACT Lantana camara (L.) is considered one of the main invasive plants, and the allelochemicals produced by this species can be defined as bioactive secondary metabolites that mediate chemical interactions between the plant and other organisms and, therefore, play an important role in its succession and establishment. The present study aimed to investigate the effect of L. camara aqueous extracts on the germination and growth behavior of Bidens pilosa (L.) by evaluating physiological parameters (germination rate, germination speed index, seedling performance and structure and membrane integrity), as well as the extract metabolic profile. The extract reduced the germination speed index and seedling growth rate and promoted abnormalities in the geotropism and root development in both populations. The third germination phase was highly affected (more than the phases I and II). Lantadene A and B are the possible compounds conferring the herbicidal properties, and the L. camara extract has a potential to be used in the development of a new environmentally-friendly herbicide.

A Cytotoxic Compound from n-Hexane Fraction of Lantana camara Linn Leaves

In this study, one triterpenoid compound from n-hexane fraction of Lantana camaraLinn leaves has cytotoxic activity was isolated. Isolation was carried out using gravity chromatographycolumnand purification by recrystallization method. Isolated compound was obtained white solid with melting point 252-253oC. The structure of isolated compound was elucidated using spectroscopic analysis Ultraviolet (UV), Infrared (IR), 1H-Nuclear Magnetic Resonance (1H-NMR), 13C-Nuclear Magnetic Resonance (13C-NMR) Heteronuclear Multiple Bond Connectivity (HMBC), Heteronuclear Multiple Quantum Correlation (HMQC), Distortionless Enhancement Polarization Transfer (DEPT) and comparative literature data, identified as Lantadene A (22β-angeloyloxy-3-oxoolean-12-en-28-oic-acid) with molecule formula C35H52O5. The Lantadene A compound was evaluated for cytotoxic activity against shrimp larvae Artemia SalinaLeach using Brine Shrimp Lethality Test(BSLT) method, showed strong cytotoxic activity with an LC50value of 48.97 µg/mL.

Labia and Lingua Histopathology of Bali Cow (Bos sondaicus) on Hepatogenous Photosensitization Symptoms in Pakutatan Village, Jembrana, Bali

Hepatogenous photosensitization is one of the ruminant diseases with symptoms of dermatitis or eczema of the skin accompanied by liver damage. The disease is caused by the compounds of toxic lantadene A and lantadene B which are secondary metabolites of Lantana camara plant. This research was carried out on January 2017. The material used in this study was preserved organs of the labia and lingua of dead three year old cow (Bos sondaicus). Samples were taken from Pakutatan Village, Jembrana, Bali. Identification of organ samples, histological preparation and histopathological examination were conducted at the Disease Investigation Center (DIC) 6, Denpasar, Bali. The preparation of labia and lingua samples used the paraffin method with Hematoxylin and Eosin staining. The tissue structural damages found were necrosis, inflammatory cell infiltration, congestion and tissue bleeding. The data obtained were analyzed statistically by One Way Anova test with a confidence level of 95%. Based on the results, dead Bali cow which consumed a large numbers of Lantana camara plants showed that the highest number of cell damage was at the picnotic stage of cell necrosis (cell death) in the labia organ significantly (P<0.005), while the highest bacterial invasion was found in the labia organ with an average percentage of 12.40%.

DOCKING MOLEKULER SENYAWA METABOLIT SEKUNDER Lantana camara SEBAGAI ANTIINFLAMASI TERHADAP ENZIM COX-1

Lantana camara mengandung beberapa jenis flavonoid seperti Umuhengerin, Lantadene A, Lantadene B, Ictero -genin. Salah satu mekanisme kerja flavonoid sebagai antiinflammatoryis dengan menghambat ceptor siklooksigenase (COX). Penelitian ini bertujuan untuk mengetahui afinitas senyawa flavonoid yang terkandung dalam L. camara dengan reseptor COX-1 sebagai antiinflamasi dan untuk mengetahui ikatan hidrogen yang dapat dibentuk dengan metode molecular docking. Pengujian in silico dilakukan berdasarkan metode desain obat yang dibantu komputer. Alat-alat yang digunakan dalam penelitian ini, perangkat lunak PyRx, perangkat lunak PyMol, Pubchem (senyawa database), PDB (Protein Data Bank), dan Po-seView. Berdasarkan hasil docking dan pembahasan di atas dapat disimpulkan bahwa Icterogenin com-pound dan senyawa Umuhengerin adalah senyawa yang paling efektif dalam proses antiinflamasi, Icterogenin com-pound memiliki nilai RMSD 41,1 Å dan nilai afinity mengikat -8,8 dan senyawa Umuhengerin memiliki val-ue RMSD 1, 61 Å dan nilai afinitas pengikatan -8.0. Ini menunjukkan bahwa senyawa tersebut memiliki nilai RMSD dari senyawa Icterogenin. Namun, senyawa Icterogenin memiliki ikatan hidrogen yang lebih kuat dan lebih efisien daripada senyawa Umuhengerin karena nilai afinitas pengikatan terendah.