Analysis on the Development Potential of Bilateral Trade Cooperation between China and Uzbekistan

The economic and trade cooperation between China and Uzbekistan has been continuously strengthened. China is Uzbekistan's largest trading partner. In order to better promote the development of Sino-Uzbek relations, this article analyzes the current situation of Sino-Uzbek bilateral trade development. In this regard, it puts forward suggestions on promoting bilateral exchanges and so on, in order to seek a broader space for cooperation between the two countries.

Effect of Sperm Cryopreservation on miRNA Expression and Early Embryonic Development

Although sperm preservation is a common means of personal fertility preservation, its effects on embryonic development potential need further investigation. The purpose of this study was to identify key microRNA (miRNA) in cryopreserved sperm and determine the changes of these miRNAs and their target genes during embryonic development using cryopreserved sperm. Moreover, the embryonic development potential of cryopreserved sperm was estimated in assisted reproductive technology (ART), where key miRNAs and target genes were validated in sperm and subsequent embryos. Clinical data of embryonic development from cryopreserved sperm indicated a significant decrease in fertilization rate in both in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) cases, as well as a reduction in blastocyst formation rate in ICSI cases. Meanwhile there was a significant increase in blocked embryo ratio of Day1, Day2, and Day3.5 embryos when frozen-thawed mouse sperm was used, compared with fresh mouse sperm, suggesting a potential negative effect of sperm cryopreservation on embryonic development. From frozen-thawed and fresh sperm in humans and mice, respectively, 21 and 95 differentially expressed miRNAs (DEmiRs) were detected. miR-148b-3p were downregulated in both human and mouse frozen-thawed sperm and were also decreased in embryos after fertilization using cryopreserved sperm. Target genes of miR-148b-3p, Pten, was identified in mouse embryos using quantitative real-time PCR (qRT-PCR) and Western blot (WB). In addition, common characters of cryopreservation of mouse oocytes compared with sperm were also detected; downregulation of miR-148b-3p was also confirmed in cryopreserved oocytes. In summary, our study suggested that cryopreservation of sperm could change the expression of miRNAs, especially the miR-148b-3p across humans and mice, and may further affect fertilization and embryo development by increasing the expression of Pten. Moreover, downregulation of miR-148b-3p induced by cryopreservation was conserved in mouse gametes.

Decentralization Reforms in Developing Countries Designed to Champion the Interests of Central Politicians and not Grassroots Development

Decentralization has been considered as a powerful tool for enhancing development. However, after nearly four decades of implementing decentralization in many countries in the Global South, the evidence suggests that the impact of decentralization on development is weak and uninspiring. This article argues that the design of a decentralization program is always critical with regard the extent to which it can promote development. The evidence across the developing countries demonstrates that the motivations behind decentralization are typically not politically neutral, political interests have been the key motivation behind the adoption of various types of decentralization programs in the developing countries. As a result, the faith in decentralization as a technical tool that could propel grassroots development needs to be reconsidered, since political interests and not development are the main determinants of decentralization. This is because, in practice, the decentralization designs in many developing countries tend to limit rather than expand the political, fiscal, and administrative powers of subnational governments, while the center retains control. This severely limits the development potential of subnational governments.

Anti-Crisis Stability of Break-Even Development Potential and its Resource Support in Agribusiness

The article considers the anti-crisis stability of the potential of break-even development and its resource support in agribusiness. The necessity of a synergetic approach to estimating the dynamic flow of resources capable of generating own sources of financing to activate the target parameters of crisis stability of break-even development potential and the development of an alternative scenario of self-financing of the production and financial cycle to stimulate economic growth of agribusiness is proved. The reproductive process of resource support of anti-crisis stability of the potential for safe development of agribusiness enterprises is substantiated. The model of estimation of target parameters of anti-crisis stability of potential of unprofitable development of agrarian business and a matrix of its point estimation at a choice of the alternative scenario of self-financing is presented. Scenarios of the flow of resource support of anti-crisis stability of the potential of unprofitable development of the agribusiness enterprise are developed. An indicator of the level of anti-crisis stability of the break-even development potential according to the determined target parameters of self-financing is offered. The dynamics of anti-crisis factor load on the stability of the potential of break-even development of agribusiness enterprises on average in one region of the Steppe zone of Ukraine by its territorial location is analyzed. Cluster analysis was used to assess the elements of the qualitative system-resource component of anti-crisis stability of the potential of break-even development with the separation of types of agribusiness enterprises in the regions of the Steppe zone of Ukraine with different structure of current assets. The forecast level of resource support according to the quantitative component of anti-crisis stability of the potential of break-even development on average in the regions of the Steppe zone of Ukraine and per one agribusiness enterprise of the region is determined. The forecast range of limits of target parameters of self-financing and their influence on the level of anti-crisis stability of potential of unprofitable development of agribusiness enterprises on the average on one region of the Steppe zone is presented

Non-invasive Metabolomic Profiling of Embryo Culture Medium Using Raman Spectroscopy With Deep Learning Model Predicts the Blastocyst Development Potential of Embryos

Purpose: This study aimed to establish a non-invasive predicting model via Raman spectroscopy for evaluating the blastocyst development potential of day 3 high-quality cleavage stage embryos.Methods: Raman spectroscopy was used to detect the metabolic spectrum of spent day 3 (D3) embryo culture medium, and a classification model based on deep learning was established to differentiate between embryos that could develop into blastocysts (blastula) and that could not (non-blastula). The full-spectrum data for 80 blastula and 48 non-blastula samples with known blastocyst development potential from 34 patients were collected for this study.Results: The accuracy of the predicting method was 73.53% and the main different Raman shifts between blastula and non-blastula groups were 863.5, 959.5, 1,008, 1,104, 1,200, 1,360, 1,408, and 1,632 cm–1 from 80 blastula and 48 non-blastula samples by the linear discriminant method.Conclusion: This study demonstrated that the developing potential of D3 cleavage stage embryos to the blastocyst stage could be predicted with spent D3 embryo culture medium using Raman spectroscopy with deep learning classification models, and the overall accuracy reached at 73.53%. In the Raman spectroscopy, ribose vibration specific to RNA were found, indicating that the difference between the blastula and non-blastula samples could be due to materials that have similar structure with RNA. This result could be used as a guide for biomarker development of embryo quality assessment in the future.

Integrated Development of Health Tourism Under the Background of Rural Revitalization Strategy

This paper studies the integrated development of forest health tourism under the background of Rural Revitalization Strategy. Based on the tourism destination life cycle theory, tourism destination competitiveness theory and experience economy theory, this paper uses the linear function weighting method to establish a measurable evaluation standard of forest health development potential. This paper constructs the evaluation index system of forest health tourism development potential through Delphi method, determines the index weight combined with analytic hierarchy process, and establishes a practical comprehensive scoring model and evaluation grade. This paper uses the fuzzy comprehensive evaluation method to evaluate the development potential of forest health tourism in three forest health bases: DWS National Forest Park, FJS National Nature Reserve and LYS National Forest Park.

Spatial distinction analysis on marine ranch development potential in coastal areas of China

According to the 14th Five-Year Plan and the outline of 2035 long-term goals, a modern marine industrial system should be built, the layout of green offshore aquaculture should be optimized, and marine ranching should be built. The research on the construction of marine ranching can provide new ideas for sustainable ecological fishery and inject new vitality into the development of marine industry. In the future, the construction of marine ranching will be a vast world. In this paper, from the perspective of geography, the GC-TOPSIS method combined with the entropy weight TOPSIS method and the grey correlation method were used to construct 22 evaluation index systems to estimate the development potential of marine ranching construction in ten coastal provinces and municipalities of China from 2011 to 2016. The results showed that the overall development potential of marine ranching in ten provinces and urban areas had regional differences (north, central and south), and the differences in the north and south regions were obvious. The regional differences in the central coastal areas of China were not significant, and the development potentials of marine ranching in ten provinces and urban areas were all medium. The development potential of four criteria layers of marine ranching in ten coastal provinces and regions of China is different in space, and the development potential of marine ranching in the same region is unbalanced in all aspects of the criteria layers. To estimate the development potential of marine ranching in China’s ten coastal provinces and regions provides scientific support for the sustainable development of ecological fisheries, and has practical application value for the scientific planning of marine ranching and the selection of demonstration areas.