calcareous fens
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2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kristie J. Schmidt ◽  
Daniel A. Soluk ◽  
Sarah E. Mays Maestas ◽  
Hugh B. Britten

AbstractDetection of environmental DNA (eDNA) has become a commonly used surveillance method for threatened or invasive vertebrates in both aquatic and terrestrial environments. However, most studies in this field favor vertebrate target species. Environmental DNA protocols can be especially useful for endangered invertebrates such as the Hine’s emerald dragonfly (Somatochlora hineana) where conservation efforts have been greatly hindered by training, time, overall costs, and environmental impacts associated with conducting surveys in the calcareous fens occupied by this species. An essential step in developing such a protocol is to evaluate the dynamics of eDNA concentration under controlled conditions. We used the quantitative polymerase chain reaction (qPCR) to examine seasonal shifts in the persistence and net-accumulation of eDNA from captive S. hineana larvae in experimental mesocosms at temperatures corresponding with their overwintering (5.0 °C) and active (16.0 °C) seasons. Environmental DNA persisted longer at 5.0 °C but accumulated more readily at 16.0 °C. Differences in the accumulation and persistence of eDNA reflect differences in the longevity of eDNA at different temperatures and seasonal differences in larval S. hineana behavior. This study highlights the importance of considering how seasonal changes in temperature influence not only the speed of eDNA degradation but also the target species’ eDNA shedding rates.


2020 ◽  
Vol 719 ◽  
pp. 134693 ◽  
Author(s):  
Michal Hájek ◽  
Veronika Horsáková ◽  
Petra Hájková ◽  
Radovan Coufal ◽  
Daniel Dítě ◽  
...  

2020 ◽  
Author(s):  
Fernando Medina Ferrer ◽  
Kathryn Hobart ◽  
Jake V. Bailey

ABSTRACTMicrobial precipitation of calcium carbonate has diverse engineering applications, from building and soil restoration, to carbon sequestration. Urease-mediated ureolysis and CO2 (de)hydration by carbonic anhydrase (CA) are known for their potential to precipitate carbonate minerals, yet many microbial community studies rely on marker gene or metagenomic approaches that are unable to determine in situ activity. Here, we developed fast and cost-effective tests for the field detection of urease and CA activity using pH-sensitive strips inside microcentrifuge tubes that change color in response to the reaction products of urease (NH3) and CA (CO2). Samples from a saline lake, a series of calcareous fens, and ferrous springs were assayed in the field, finding relatively high urease activity in lake samples, whereas CA activity was only detected in a ferrous spring. Incubations of lake microbes with urea resulted in significantly higher CaCO3 precipitation compared to incubations with a urease inhibitor. Therefore, the rapid assay indicated an on-site active metabolism potentially mediating carbonate mineralization. Field urease and CA activity assays complement molecular approaches and facilitate the search for carbonate-precipitating microbes and their in situ activity, which could be applied toward agriculture, engineering and carbon sequestration technologies.


2019 ◽  
Vol 29 (3) ◽  
pp. 709-728 ◽  
Author(s):  
Torbjörn Tyler ◽  
Stefan Andersson ◽  
Lars Fröberg ◽  
Kjell-Arne Olsson ◽  
Åke Svensson ◽  
...  

AbstractBased on data from three surveys of the vascular flora of the province of Scania, southernmost Sweden, conducted 1938–1971, 1987–2006 and 2008–2015, we analyse the change in frequency of individual species and groups of species associated with particular vegetation types. A majority of all species have experienced a change in frequency since 1938, and this turnover has continued in recent decades. The species showing the most dramatic declines since 1987 represent a mixture of arable weeds, grassland species and ruderals, but excludes forest species. In contrast, a majority of the most increasing species are escapes from cultivation that thrive under shaded conditions. The vegetation types showing the largest decreases since 1987 are all open seminatural grasslands and wetlands, while the vegetation types performing best are wooded. All vegetation types increasing since 1987 also increased during the 1900s; however, species of wooded types performed relatively better in recent decades, as opposed to the minimal increase observed for species of vegetation strongly influenced by human activities. Among decreasing vegetation types, those that have received much attention from conservationists, e.g. sand-steppe and calcareous fens tend to perform relatively better now than during the 1900s, while those that have received less attention, e.g. poor fens, oligotrophic waters and heaths, now comprise the most rapidly declining vegetation types. A majority of the species that decreased 1938–1996 also decreased 1987–2015, but, in general, species shown to have increased during the 1900s have not continued to increase.


2019 ◽  
Vol 88 (3) ◽  
pp. 277-296 ◽  
Author(s):  
Tomáš Němec ◽  
Michal Horsák

Shell formation is the main defensive strategy against predation for the majority of snails. Therefore, various predators have had to develop a variety of techniques how to overcome this barrier. As shells can persist in a calcium-rich environment for a long time, specific external or internal traces on shells left by predators indicate whether and who killed the snail. Based on litter samples collected at 30 sites of five different habitat types, the intensity and type of predation were assessed. The minimal predation rate varied between 0.0 and 21%, with an average of 8%. The highest rate was observed at limestone steppes, on average 15%. Beetles were found to be the most common predators of snails; however, predation by snails was more common in calcareous fens. Predation by some vertebrates and dipteran flies was also recognised. To test the role of mouth barriers as a means to reduce predation by carabid beetles that break the shell from an aperture, we analysed the predation rate separately on adult and juvenile shells using 24 populations of the steppe snail Granaria frumentum (Draparnaud, 1801). As expected, carabid beetles chiefly preferred juveniles compared to adult shells (Wilcoxon test, p < 0.001). On the contrary, the parasitoid fly Pherbellia limbata (Meigen, 1830) and Drilus beetles preferred adults. We found that predation by carabid beetles positively increased with prey abundance (R2 = 42.8%, p = 0.021), while no relation was observed for the parasitoid (p = 0.703), likely due to their feeding specialisation.


2019 ◽  
Vol 54 (1-2) ◽  
pp. 125-138 ◽  
Author(s):  
Leonid Rasran ◽  
Cornelia Eisenmann ◽  
Regina Wagentristl ◽  
Karl-Georg Bernhardt

AbstractPlant species of the intermediate life strategy type are characteristic for species-rich grasslands, which are sensitive to changes in the level of disturbance. Germination and early establishment of seedlings is expected to be the most crucial stage, limiting the successful colonization of appropriate sites. Valeriana dioica is a typical plant of calcareous fens, which are dependent on regular suppression of strong competitors by means of moderate land use. It is endangered in many parts of Central Europe, including Lower Austria. We studied the effects of light, temperature and pre-treatment (cold stratification) on the germination success of V. dioica seeds and the role of litter produced by different competitors on the early establishment of seedlings. We tested seeds from five populations differing in the level of competition for light and considered also the morphological variability of propagules and maternal plants. We found that V. dioica is able to germinate without light. It showed high germination percentages both under high summer temperatures independent of cold stratification and at low temperatures, but only after the stratification. Litter produced by dicotyledonous forbs was less hampering for the seedlings than that of small sedges or grasses. Valeriana dioica possesses a broad regeneration niche and is able to use gaps for germination and establishment both in spring and in summer. It reproduces successfully in herb-dominated fen meadow vegetation but disappears from reed communities. This study demonstrates the significant plasticity of the regeneration niche for a species with an intermediate life strategy and also stresses the importance of gaps for its reproduction.


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