culture strategy
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Development ◽  
2022 ◽  
Author(s):  
Ling Yu ◽  
Yu-Lieh Lin ◽  
Mingquan Yan ◽  
Tao Li ◽  
Emily Y. Wu ◽  
...  

Amputation injuries in mammals are typically non-regenerative, however joint regeneration is stimulated by BMP9 treatment (Yu et al., 2019) indicating the presence of latent articular chondrocyte progenitor cells. BMP9 induces a battery of chondrogenic genes in vivo, and a similar response is observed in cultures of amputation wound cells. Extended cultures of BMP9 treated cells results in differentiation of hyaline cartilage and single cell RNAseq analysis identified wound fibroblasts as BMP9 responsive. This culture model was used to identify a BMP9 responsive adult fibroblast cell line and a culture strategy was developed to engineer hyaline cartilage for engraftment into an acutely damaged joint. Transplanted hyaline cartilage survived engraftment and maintained a hyaline cartilage phenotype but did not form mature articular cartilage. In addition, individual hypertrophic chondrocytes were identified in some samples indicating that the acute joint injury site can promote osteogenic progression of engrafted hyaline cartilage. The findings identify fibroblasts as a cell source for engineering articular cartilage and establishes a novel experimental strategy that bridges the gap between regeneration biology and regenerative medicine.


2021 ◽  
Vol 5 (2) ◽  
pp. 49-56
Author(s):  
Dian Rokhmawati ◽  
Rachma Yuliana

Conflicts in family companies cannot be avoided, given that there are stronger emotional factors in employee family ties that are integrated with logic and rational targets in doing business. For this reason, a conflict management strategy is needed that makes conflict a supporter of the strength of the family business. This study aims to: 1) Determine the extent to which conflict affects the activities and business performance of the company, and 2) how the strategy of PT. Book Mart Indonesia in managing conflicts that occur. The approach used in this research is qualitative, while the type of research is descriptive. The determination of the source in this study was carried out by means of purposive sampling. Two types of data used are primary data and secondary data. Data collection procedures used were interviews and documentation. The results showed that the root causes of conflict in PT. Book Mart Indonesia (PTBMI) is a company that is empty of family values, no organizational culture and weak corporate vision and mission. Conflicts that occur (media.neliti.com), for example misunderstandings related to information, decisions related to project making and decisions about task shifting / division of labor. This conflict is caused by the absence of Standard Operating Procedures (SOP), the absence of clear company rules such as an administrative system (recording), professional financial management to strengthening brands in the market. This conflict has a major impact, with indications of high employee turnover and a decline in company performance. Keywords : Conflict, Family Company, Organizational Culture, Strategy, Management.


Foods ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 2098
Author(s):  
Sheng-Yao Wang ◽  
Ren-Feng Huang ◽  
Ker-Sin Ng ◽  
Yen-Po Chen ◽  
Jia-Shian Shiu ◽  
...  

Our previous studies indicated that Lactobacillus kefiranofaciens HL1, isolated from kefir grain, has strong antioxidant activities and anti-aging effects. However, this strain is difficult to use in isolation when manufacturing fermented products due to poor viability in milk. Thus, the purpose of this study was to apply a co-culture strategy to develop a novel probiotic fermented milk rich in L. kefiranofaciens HL1. Each of four selected starter cultures was co-cultured with kefir strain HL1 in different media to evaluate their effects on microbial activity and availability of milk fermentation. The results of a colony size test on de Man, Rogosa and Sharpe (MRS) agar agar, microbial viability, and acidification performance in MRS broth and skimmed milk suggested that Lactococcus lactis subsp. cremoris APL15 is a suitable candidate for co-culturing with HL1. We then co-cultured HL1 and APL15 in skimmed milk and report remarkable improvement in fermentation ability and no negative impact on the viability of strain HL1 or textural and rheological properties of the milk. Through a co-culture strategy, we have improved the viability of kefir strain HL1 in fermented skimmed milk products and successfully developed a novel milk product with a unique flavor and sufficient probiotics.


2021 ◽  
Vol 32 ◽  
pp. S370
Author(s):  
G.T. Ulu ◽  
N.N. Bayram ◽  
S. Dinçer İşoğlu ◽  
Y. Baran

2021 ◽  
Vol 9 (1) ◽  
pp. 16-30
Author(s):  
Sara Scipioni

As a dynamic phenomenon that interacts across different levels – individual, group, organizational, interorganizational – the development of a unique multilevel theory of Organizational Learning (OL) is absent and challenging. The intent of this paper is to contribute to the advancement of such a theory. In this context, a systematic review of the 2004-2020 literature was carried out, with analysis of 120 papers selected from management and organization science top-ranked journals. Based on the conceptualization of OL as multiple processes of knowledge creation, transfer, and retention, the reviewed papers highlight that internal and external environments, organizational culture, strategy, structure, leadership, technology, and shared environments need to be considered for a comprehensive understanding of vertical trickle-down OL processes, and of bottom-up emerging OL processes, in both single and multi-level OL analyses. This study contributes to the theory of OL with the presentation of a novel taxonomy of contextual factors that could help researchers in the development of comprehensive OL studies. The implications offered should support the definition of a multilevel theory for OL that embraces all the relevant factors that influence its processes across the different organizational levels. The review closes with specific recommendations for further studies in OL.


2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
C Alber. Rodriguez ◽  
M Valera ◽  
L Bori ◽  
F Meseguer ◽  
L Alegre ◽  
...  

Abstract Study question Is there a significant difference in the clinical results of embryos cultured in time-lapse systems with single-step medium and conventional benchtop incubators with sequential media? Summary answer Embryos cultured in time-lapse systems and single-step media are more likely to achieve an ongoing pregnancy and have higher implantation rates than those cultured otherwise. What is known already One of the strategies for embryo culture in IVF consisted in conventional benchtop incubators combined with sequential culture media (CI-Seq). New generation time-lapse systems provide useful information on the morphokinetics of embryo development, but also a stable culture environment where embryos can develop undisturbed until blastocyst stage when paired with single-step culture media (TLS-SS). These features have the potential to improve embryo development and selection. Nonetheless, there is inconclusive evidence of whether this new culture strategy has a significant effect on clinical results of ICSI treatments. Studies on the matter are heterogeneous and reduced in both number and sample size. Study design, size, duration Unicentric retrospective cohort study. We compared the results of 11471 blastocyst transferences from 10276 ICSI treatments performed during 4 consecutive years, where embryos were cultured either on CI with sequential media (N = 5255) or a TLS with single-step medium (N = 5021). 3922 of the totals were fresh embryo transfers (ET) and 7549 frozen-thawed ET. We compared the implantation rate (IR) and ongoing pregnancy rate (OGPR) in both study groups, stratifying by ovum origin. Participants/materials, setting, methods Three models of TLS were used for embryo culture: EmbryoScope, EmbryoScope Plus (Vitrolife) and GERI (Genea Biomedx), as well as one CI (ASTEC). Sequential media: Cook, Origio, Vitrolife; Single-step media: Gems, Irvine, Life Global. Embryo scoring and selection was performed by ASEBIR criteria in the CI group, and by morphological and morphokinetic assessment for embryos cultured in TLS. Embryos were extracted from the CI only for media change. Statistical analysis: ANOVA tests and Logistic regressions. Main results and the role of chance A general Logistic Regression was performed, including egg origin, PGT-A and culture strategy to explain their impact in OGPR. Egg origin (OR = 1,094 (95%CI: 1,015–1,179); P = 0,019) and culture strategy (OR = 1,141 (95%CI: 1,060–1,229); P < 0,001) were statistically significant, which confirms the need for stratification due to the heterogeneity of the groups. The total IR in the TLS-SS group was 54,68±48,84%, significantly higher than that of CI-Seq (49,18±47,91%; P < 0,001). In ovum-donation treatments, a complete Logistic Regression for OGPR, with all typical confounding variables (age, BMI, nº oocytes, fresh/frozen transfer, number and day of ET) resulted in an OR = 1,187 (95%CI: 1,074–1,313; P = 0,001) favoring culture in TL-SS. IR in these treatments were 61,98±47,68% in TL-SS vs 55,08±46,58% in CI-Seq (P < 0,001) in fresh transfers and 51,48±48,91% in TL-SS vs 44,39±47,67% in CI-Seq (P < 0,001) in frozen-thawed ET. In autologous treatments with PGT a similar regression yielded an OR = 1,055 (95%CI: 0,889–1,252; P = 0,542) for culture strategy. The IR of genetically tested ET was not significantly different: 53,08±49,49% for TL-SS, 50,90±49,07% for CI-Seq, P = 0,246. In autologous procedures without PGT, culture strategy was not significant for OGPR (OR = 0,998 (95%CI: 0,835–1,191), P = 0,979) nor IR of fresh (49,75±48,91% TL-SS vs 44,23±47,36% CI-Seq; P = 0,081) nor frozen-thawed transferences (50,77±48,33% TL-SS vs 50,67±47,33% CI-Seq; P = 0,970). Limitations, reasons for caution After fertilization check, embryos were evaluated exclusively on D5/6. On D3, embryos cultured in CI were taken out only for a quick media change, but not for evaluation, and all handling was done in isolette cabins with controlled environmental conditions. Being a retrospective study, there is high variability in population. Wider implications of the findings: A more homogenous prospective study, including comparison in life-birth rates, is necessary to extract final conclusions. However, our results suggest that the introduction of TLS and SS media in IVF laboratories might be a valid strategy to increase clinical results, especially in fresh embryo, thanks to an improved embryo selection. Trial registration number Not applicable


2021 ◽  
pp. 125579
Author(s):  
Ming Xu ◽  
Dong ou ◽  
Zhaoxia Xue ◽  
Yongjun Zhao ◽  
Shiqing Sun ◽  
...  
Keyword(s):  

2021 ◽  
Vol 12 ◽  
Author(s):  
Rou-Wen Chen ◽  
Yuan-Qiu He ◽  
Lin-Qing Cui ◽  
Cun Li ◽  
Song-Biao Shi ◽  
...  

Actinobacteria are ubiquitous in marine ecosystems, and they are regarded as an important, underexplored, potential pharmaceutical resource. The orders Gaiellales and Rubrobacterales are deep taxonomic lineages of the phylum Actinobacteria, both are represented by a single genus and contain only a few species. Although they have been detected frequently by high-throughput sequencing, their functions and characteristics in marine habitats remain unknown due to the lack of indigenous phenotypes. Here, we investigated the status of the orders in South China Sea (SCS) sediments using culture-independent and culture-dependent methods. Gaiellales is the second-most abundant order of Actinobacteria and was widely distributed in SCS sediments at water depths of 42–4,280 m, and four novel marine representatives in this group were successfully cultured. Rubrobacterales was present at low abundance in energy-limited marine habitats. An isolation strategy for Rubrobacterales from marine samples was proposed, and a total of 138 mesophilic Rubrobacterales strains were isolated under conditions of light and culture time combined with high-salinity or low-nutrient media. Marine representatives recovered in this study formed branches with a complex evolutionary history in the phylogenetic tree. Overall, the data indicate that both Gaiellales and Rubrobacterales can adapt to and survive in extreme deep-sea environments. This study lays the groundwork for further analysis of the distribution and diversity of the orders Gaiellales and Rubrobacterales in the ocean and provides a specific culture strategy for each group. The results open a window for further research on the ecological roles of the two orders in marine ecosystems.


2021 ◽  
Author(s):  
Xianguang Nie ◽  
Lin Wang

Abstract Bisphenol A (BPA), a broadly disseminated endocrine disturbing chemicals in environment, is harmful to creatures and plants. Plants can uptake and metabolize BPA, but a single plant species ability is limited. Undeniably, plant species compositions have a more vital ability to remove pollutants than a single plant species. However, the mechanisms of plant species compositions alleviating toxicological effects of bisphenol A are poorly understood. Here, we administered plant species compositions, which based on a full-factorial design of Phragmites australis(A), Typha latifolia(B) and Arundo donax(C), to unveil their role in BPA exposure. The comes about illustrated that with 1.5-10mg L−1 of BPA introduction, the mixed-hydroponic culture groups (e.g. sp(ABC)) significantly increased biomass production and photosynthetic pigments content as revealed by augmented the shoots fresh, dry weight, chlorophyll a and total chlorophyll content. While mixed-hydroponic culture groups (e.g. sp(AB), sp(ABC)) significantly increased antioxidant enzymes activity and antioxidant substances, it astoundingly diminished responsive oxygen species (ROS) and malondialdehyde (MDA) substance, proposing that mixed-hydroponic culture groups calmed oxidative stretch in takes off. Further analysis revealed that mixed-hydroponic culture groups (e.g. sp(AB), sp(AC), sp(ABC)) significantly increased detoxification enzyme activity of NADPH-cytochrome P450 reductase (CPR), glutathione S-transferase (GST) and glycosyltransferase (GT). Moreover, mixed-hydroponic culture groups (e.g. sp(AB), sp(AC), sp(ABC)) decreased the BPA substance in leaves, proposing that mixed-hydroponic culture groups advanced BPA metabolism by improving CPR, GST, GT detoxification. These results suggest that a mixed-hydroponic culture strategy can alleviate BPA phytotoxicity and possibly offer natural and potential phytoremediation of BPA way.


2021 ◽  
pp. 25-31
Author(s):  
G. Zi

The article theoretically substantiates the importance of the category “management culture” as part of the organizational culture in the formation and implementation of the organizations strategy and separately considers some of its elements. The paper presents the categories and subcategories of management culture: strategy, organizational structure, regulation, technology, information systems, control, incentives. The study analyses the importance of the strategy as one of the elements of the management culture and the ratio of goals. The paper evaluates the factors that determine the success of planning and implementing the strategy. The article pays special attention to the discussion of information technologies as one of the elements of management culture, intensity and options for the development and implementation of modern technologies in business process management. When discussing processes as one of the elements of the management culture, the author emphasizes the complexity of managing processes and the importance of their consistency for achieving the goals of the organization. The study concludes, that the main role of management is to achieve the goals of the company using a certain number of employees, management methods and controls, which are regulated by the management culture, which is an integral part of the organizational culture of the company. 


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