Emerging Advances of Detection Strategies for Tumor-Derived Exosomes

Exosomes derived from tumor cells contain various molecular components, such as proteins, RNA, DNA, lipids, and carbohydrates. These components play a crucial role in all stages of tumorigenesis and development. Moreover, they reflect the physiological and pathological status of parental tumor cells. Recently, tumor-derived exosomes have become popular biomarkers for non-invasive liquid biopsy and the diagnosis of numerous cancers. The interdisciplinary significance of exosomes research has also attracted growing enthusiasm. However, the intrinsic nature of tumor-derived exosomes requires advanced methods to detect and evaluate the complex biofluid. This review analyzes the relationship between exosomes and tumors. It also summarizes the exosomal biological origin, composition, and application of molecular markers in clinical cancer diagnosis. Remarkably, this paper constitutes a comprehensive summary of the innovative research on numerous detection strategies for tumor-derived exosomes with the intent of providing a theoretical basis and reference for early diagnosis and clinical treatment of cancer.

Improving the Performance of Mortars Made from Recycled Aggregates by the Addition of Zeolitised Cineritic Tuff

Metropolitan construction and demolition waste (CDW) is currently an important source of recycled materials that, despite having completed their useful life cycle, can be reincorporated into the circular economy process (CEP); however, the recycling process is very selective, and waste material is not always fully satisfactory due to the intrinsic nature of the waste. This work aims to demonstrate and establish how to increase the effectiveness of the construction and demolition waste in more resistant mortars, by mixing it with zeolitised cinerite tuff (ZCT) at varying normalised proportions. To attain the objectives of this research, a series of tests were done: First, a chemical, physical and mineralogical characterisation of the CDW and the ZCT through XRF, XRD, SEM and granulometric methods. Second, a technological test was made to determine the mechanical strength at 7, 28 and 90 days of specimens made with Portland cement (PC) and mixtures of PC/CDW, PC/ZCT, and PC/CDW-ZCT. The results obtained through the characterisation methods showed that the sample of construction and demolition waste consisted of the main phase made of portlandite and tobermorite, and by a secondary phase consisting of quartz, ettringite and calcite; whereas the ZCT has a main phase of mordenite and a secondary phase of smectite (montmorillonite), amorphous materials consisting of devitrified volcanic glass, quartz and plagioclase. Mechanical strength tests established that specimens made with PC/CDW mixtures have very discreet compressive strength values up to 44 MPa at 90 days, whereas specimens made with PC/ZCT mixtures achieved a remarkably high mechanical strength consisting of 68.5 MPa. However, the most interesting conclusion in this research is the good result obtained in mechanical strength of the specimens made up of mixtures of PC/CDW-ZCT, which increased from 52.5 to 62 MPa at 90 days of curing; this fact establishes the positive influence of ZCT on waste in the mortar mixtures, which permits the authors to establish that the objective of the work has been fulfilled. Finally, it can be argued that the results obtained in this research could contribute to more effective use of construction and demolition waste in metropolitan areas.

Single-Cells Isolation and Molecular Analysis: Focus on HER2-Low CTCs in Metastatic Breast Cancer

Although the detection of CTCs expressing HER2 at low intensity (HER2-low CTCs) has been shown to have a negative prognostic value in metastatic breast cancer (MBC) patients, the biological intrinsic nature of HER2-low CTCs remains unexplored. Considering the technical challenges behind the selective collection of immunophenotype-specific CTCs, we developed a pipeline to individually capture HER2-low CTCs. Four different breast cancer cell lines (MDA-MB-231, T47D, MDA-MB-453, and SKBR3), that are known to express HER2 at different immunohistochemistry levels (respectively classified as 0, 1+, 2+, and 3+), were spiked in healthy donor blood tubes (7.5 mL) and processed with the CellSearch® (Menarini Silicon Biosystems, Bologna, Italy) for enrichment and the DEPArray NxT™ for single cell selection. The HER2 signal-intensities of each cell line was compared using the nonparametric Mann–Whitney U test. The optimal cut-offs to distinguish HER2 1+ from 0 and 2+ cells were calculated performing the Receiver operating characteristic (ROC) curve. Median HER2 signal-intensities detected with the DEPArray NxT™ were: 2.59 (0), 3.58 (1+), 5.23 (2+) and 38.37 (3+). DEPArray NxT efficiently differentiated each single cell line (p < 0.001). The area under the ROC curve was 0.69 and 0.70 (respectively 0 vs. 1+ and 1+ vs. 2+) and the optimal calculated cut-offs were 2.85 (lower) and 4.64 (upper). HER2-low CTCs can be detected and separately collected using predetermined intensity cut-offs. This study will allow standardized single-cell or pooled collection of HER2-low CTCs for downstream molecular analyses.

The institutional evolution of central banks

This article contributes to the literature on central banks’ institutional rationale and evolution by analyzing the early development of the Bank of England as a case study. The history of the Bank is scrutinized under the framework of entangled political economy, revealing its origins in a process of bank and political bargains. The account clarifies the process by which the political and economic order becomes increasingly intertwined throughout the banking system, via political bargains under incomplete contracts. The analysis suggests that entanglement allows governments and non-profit organizations to transmit some of their features to banking organizations in exchange for financial benefits. Transmitting nonmarket characteristics through recurrent bargains leads a for-profit bank to gradually transform into a central bank. The article proposes an alternative rationale for the unintended emergence of central banks, providing evidence in favor of their politically oriented development, rather than their alleged intrinsic nature.

Cultural Diversity: Aesthetic Analysis of Persian and Kashmiri Paisley Ornaments

The article focuses on the origins and evolution of the Indo-Persian decoration "Paisley," as well as the use of this flowery linear pattern on textiles such as shawls, table wraps, and carpets. Paisley has gone through many stages in its evolution, including the Iranian art period and the Indo-Subcontinent phase (Mughal era). The intrinsic nature of the decoration is shown via a comparative research technique that defines Indo-Persian motif features. This article looked at the primary ornamental elements of the paisley pattern, which are floral and geometrical in nature, and how they are used in Iran and Kashmir. Finally, this article discusses how, because of Paisley's growing and blooming character, it is often a prominent ornament in the most important works of enrichment and plays an accentuation role in the decoration of textile surfaces. Paisley, a masterpiece, is especially linked to techniques for conveying the aesthetic brilliance of the pattern used in many cultures.

Observation of giant exchange bias effect in Ni-Mn-Ti all-d-metal Heusler alloy

We report a giant exchange bias (EB) field of about 3.68 KOe during field cooled process in all-d-metal Ni40(FeCo)4Mn36Ti20 Heusler alloy. The study of magnetic memory effect and isothermal magnetic relaxation processes suggest that the giant EB field arises due to the possible coexistence of antiferromagnetic (AFM) and ferromagnetic (FM) phase exchange interaction in the studied system at temperatures below 35 K. Furthermore, the temperature and cooling field dependence of EB effect are analyzed which are related to the change in unidirectional anisotropy at FM/AFM interface. The study of a well-established training effect confirms the intrinsic nature of the observed EB behavior. This result will open up a new way towards the development of EB materials considering all-d-metal Heusler alloy systems.

Impact of COVID-19 Measures on a Paediatric Oncology Outreach Program: Medical Perspectives

Background Paediatric oncology outreach programs have been effective development interventions to reduce inequalities in healthcare between high-income countries (HIC) versus low and middle-income countries (LMIC). But little is known about its sustainability during times of a pandemic. This study assesses the impact of COVID-19 government measures on a paediatric oncology outreach program between three large referral hospitals in the Netherlands, Indonesia and Kenya. Methods The head from each paediatric oncology outreach partner site was interviewed using a semi-structured questionnaire in June 2021. Results COVID-19 government measures impacted childhood cancer care at all three hospitals. However, disruptions in services are more prominent at partner sites in LMIC, increasing existing inequalities.The doctor from Dutch hospital, located in a HIC, reported only one disruption which is a decrease in surveillance evaluations for patients who completed cancer treatment. The doctors from Indonesian and Kenyan hospitals, located in LMIC, reported multiple disruptions. For example, reduced number of cancer diagnoses, delayed presentations, medical staff shortages, limited availability of chemotherapy and blood products, and delayed or modified treatment administration. Physical interaction between medical teams of all three participating institutions has slowed down. Hereby, adequate transfer of knowledge, skills and expertise have been adversely affected. Conclusion COVID-19 government measures have negatively impacted the intrinsic nature of the paediatric oncology outreach program. Disruptions in childhood cancer care services are more prominent at partner sites in LMIC. Government leaders and policy makers should take collateral damage of their policies and local settings into account to protect children with cancer

Anti-Realisms Local and Global

The Madhyamaka school of Buddhist philosophy rejects the metaphysical realist thesis that there is such a thing as how things are anyway, independently of the concepts we happen to use. While other Buddhist schools claim that such things as persons, trees, and tables are mere useful fictions, they maintain that these fictions are usefully grounded in facts about the ultimate nature of reality. Not so Mādhyamikas, who are pan-fictionalists. They support this stance by seeking to demonstrate that various hypotheses concerning things with intrinsic nature (things that are thus purported to be ultimately real) lead to absurdities. The conclusion one is invited to draw is that all things are empty, that is, devoid of intrinsic nature. Some sample reductios are examined, and various realist objections are considered. Several different interpretations of Madhyamaka anti-realism are discussed, with a semantic non-dualist reading, to the effect that the very idea of ultimate truth is incoherent, being judged the most plausible.