optical techniques
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2021 ◽  
Author(s):  
Juan Manuel Madariaga ◽  
Roger Wiens ◽  
Gorka Arana ◽  
Violaine Sautter ◽  
Karim Benzerara ◽  
...  

2021 ◽  
Author(s):  
Juan Manuel Madariaga ◽  
Roger Wiens ◽  
Gorka Arana ◽  
Violaine Sautter ◽  
Karim Benzerara ◽  
...  

2021 ◽  
Author(s):  
Juan Manuel Madariaga ◽  
Roger Wiens ◽  
Gorka Arana ◽  
Violaine Sautter ◽  
Karim Benzerara ◽  
...  

Materials ◽  
2021 ◽  
Vol 14 (24) ◽  
pp. 7502
Author(s):  
Polina S. Tseregorodtseva ◽  
Kirill E. Buiankin ◽  
Boris P. Yakimov ◽  
Armais A. Kamalov ◽  
Gleb S. Budylin ◽  
...  

Diffuse reflectance spectroscopy (DRS) and imaging are increasingly being used in surgical guidance for tumor margin detection during endoscopic operations. However, the accuracy of the boundary detection with optical techniques may depend on the acquisition parameters, and its evaluation is in high demand. In this work, using optical phantoms with homogeneous and heterogeneous distribution of chromophores mimicking normal and pathological bladder tissues, the accuracy of tumor margin detection using single-fiber diffuse reflectance spectroscopy and spatial frequency domain imaging was evaluated. We also showed how the diffuse reflectance response obtained at different spatial frequencies with the spatial frequency domain imaging technique could be used not only to quantitatively map absorption and scattering coefficients of normal tissues and tumor-like heterogeneities but also to estimate the tumor depth localization. The demonstrated results could be helpful for proper analysis of the DRS data measured in vivo and for translation of optical techniques for tumor margin detection to clinics.


2021 ◽  
Vol 11 (23) ◽  
pp. 11433
Author(s):  
Werner B. Herppich ◽  
Tamás Zsom

Green-ripe banana fruit are sensitive to chilling injury (CI) and, thus, prone to postharvest quality losses. Early detection of CI facilitates quality maintenance and extends shelf life. CI affects all metabolic levels, with membranes and, consequently, photosynthesis being primary targets. Optical techniques such as chlorophyll a fluorescence analysis (CFA) and spectroscopy are promising tools to evaluate CI effects in photosynthetically active produce. Results obtained on bananas are, however, largely equivocal. This results from the lack of a rigorous evaluation of chilling impacts on the various aspects of photosynthesis. Continuous and modulated CFA and imaging (CFI), and VIS remission spectroscopy (VRS) were concomitantly applied to noninvasively and comprehensively monitor photosynthetically relevant effects of low temperatures (5 °C, 10 °C, 11.5 °C and 13 °C). Detailed analyses of chilling-related variations in photosynthetic activity and photoprotection, and in contents of relevant pigments in green-ripe bananas, helped to better understand the physiological changes occurring during CI, highlighting that distinct CFA and VRS parameters comprehensively reflect various effects of chilling on fruit photosynthesis. They revealed why not all CFA parameters can be applied meaningfully for early detection of chilling effects. This study provides relevant requisites for improving CI monitoring and prediction.


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