THE USE OF CARROT SEED OIL (DAUCUS CAROTA L.) TO FORMULATE NANOEMULGELS AS AN EFFECTIVE NATURAL SUNSCREEN AND SKIN ANTI-AGING

Objective: The purpose of this study was to develop a nanoemulgel containing vegetable oil of carrot seed oil as an effective natural sunscreen and skin anti-aging. Methods: Nanoemulgels containing 4% carrot seed oil were formulated in three formulas with different ratios of Tween 80 and Sorbitol and prepared by using the high-energy emulsification method. The nanoemulgels were determined for the organoleptic characteristic, globule size, pH, physical stability during storage for 12 w at three different temperatures (room, high and low temperature), centrifugation, and cycling test. The Sun Protection Factor (SPF) value was determined by UV spectrophotometric method and the effectiveness of anti-aging was evaluated by using a skin analyzer and the results were compared with sunscreen emulgel. Results: Nanoemulgel containing 4% carrot seed oil with a ratio of Tween 80 as surfactant and Sorbitol as co-surfactant 40 and 20 resulted in the smallest mean droplet size of 338.34 nm and the sizes were increased during 12 w of storage at room temperature but still in the nano size and this nanoemulgel did not show phase separation or still stable. These nanoemulgels were also stable after the centrifugation and cycling test. The emulgel preparation was not stable or showed phase separation after the centrifugation test. The SPF value obtained from the nanoemulgel was 20.28±0.22 and these values were higher than the sunscreen emulgel (13.94±0.27). The pore size, spot, and wrinkles of the volunteer skin were reduced after using the nanoemulgel containing 4% carrot seed. Conclusion: The sunscreen and skin anti-aging activity of nanoemulgel preparation containing 4% carrot seed oil with a ratio of surfactant Tween 80 and co-surfactant Sorbitol 40 and 20 were more effective compare with emulgel preparation.

Formulasi Krim Lulur Scrub dari Ekstrak Etanol Ubi Jalar Ungu (Ipomoea batatas (L.) Lam.) dan Serbuk Beras Putih (Oryza sativa L.)

Umbi ubi jalar ungu (Ipomoea batatas (L.) Lam.) dan beras putih (Oryza sativa L.) diketahui memiliki aktivitas antioksidan. Penelitian ini bertujuan untuk membuat sediaan krim lulur scrub dari ekstrak etanol ubi jalar ungu dan mengetahui formula yang paling disukai. Umbi ubi jalar ungu diekstraksi dengan pelarut etanol 70% hingga menjadi ekstrak kental. Ekstrak kental tersebut kemudian diformulasikan menjadi sediaan krim lulur scrub yang terbagi atas 3 formula dengan variasi konsentrasi ekstrak yaitu konsentrasi 2% (FI), 4% (FII) dan 6% (FIII) yang masing masing formula ditambahkan serbuk beras putih sebagai bahan scrub. Evaluasi krim lulur scrub meliputi evaluasi mutu fisik (uji organoleptis, homogenitas, tipe krim, daya sebar, viskositas, sifat alir), uji pH, uji stabilitas dipercepat dengan metode cycling test dan uji hedonik (aroma, tekstur, warna, kesan lengket). Hasil evaluasi mutu fisik dan uji stabilitas menunjukkan krim lulur scrub yang diperoleh memiliki mutu dan stabilitas fisik yang baik. Hasil uji hedonik menunjukkan formula I merupakan formula yang paling disukai.

UJI STABILITAS FISIK FORMULASI SEDIAAN LIP BALM YANG MENGANDUNG EKSTRAK ETANOL BUAH LABU KUNING (Curcubita moschata D.)

Bibir merupakan salah satu bagian wajah yang penampilannya mempengaruhi persepsi estetika wajah. Ekstrak etanol buah labu kuning (Curcubita moschata D.) telah diakui memiliki aktivitas antioksidan dengan menggunakan metde DPPH. Pada penelitian ini, 2%, 4% dan 8% ekstrak etanol buah labu kuning diformulasikan dalam bentuk lip balm karena berguna untuk melindungi dan menjaga kelembaban bibir. Tujuan penelitian ini adalah untuk mengetahui stabilitas fisik formulasi sediaan lip balm yang mengandung ekstrak etanol buah labu kuning. Uji stabilitas fisik dilakukan uji organoleptis, homogenitas, pH, suhu lebur dan cycling test. Hasil penelitian menunjukkan bahwa ekstrak etanol buah labu kuning dapat diformulasikan dalam sediaan lip balm yang memiliki susunan yang homogen, pH yang sesuai (5,8-6,3), suhu lebur yang baik (55-59 ) dan stabil selama cycling test. Sediaan lip balm ekstrak etanl buah labu kuning dengan konsentrasi 8% memiliki stabilitas fisik yang paling baik.

Uji Formulasi dan Uji Efektivitas Repelan Lotion Ekstrak Daun Tembakau (nicotiana tabacum. L) Terhadap Nyamuk Aedes Aegypti

Tobacco leaves (Nicotiana tabaccum. L) have great potential as a pesticide or insect repellent, especially the Aedes aegypti mosquito. Maceration method was used to obtain tobacco leaf extract as much as 127.86 grams from the dried simplicia of tobacco leaves (Nicotiana tabaccum. L) 827 grams (yield 15.46%). The tobacco extract was made in the form of lotio with a concentration of 1%, 2%, and 3%, having repellant effectiveness of 90%, 96% and 100%, respectively. Physical stability test was carried out on the lotion 0%, 1%, 2%, and 3% as follows; organoleptic test (brownish color, typical tobacco aroma and homogeneity), spreadability test (6.1cm; 6.1cm; 6.2cm; 6.4cm), adhesion test (3.06sec; 2.8sec; 2.75sec; 2.61sec), cycling test (brownish color, thick consistency, homogeneity, and distinctive aroma of tobacco), pH test (7; 7; 7; 6,3), and viscosity test (4.708cPs, 4.575cPs, 4.465cPs, 4.403cPs). The results of One Way ANOVA analysis showed that there was a significant difference in each concentration of tobacco leaf extract lotio and Tukey's test showed that the repellant ability of 3% concentration had 100% percent repelance. It can be concluded that the ethanol extract lotion of tobacco leaves (Nicotiana tabaccum. L) has repellant effectiveness.Keywords: tobacco leaves, extract, lotion, repellant, ANOVA AbstrakDaun tembakau (Nicotiana tabaccum. L) memiliki potensi besar sebagai pestisida atau pengusir serangga khususnya nyamuk Aedes aegypti. Metode maserasi digunakan untuk mendapatkan ekstrak daun tembakau sebanyak 127,86 gram dari simplisia kering daun tembakau (Nicotiana tabaccum. L) 827 gram (rendemen 15,46%). Ekstrak tembakau dibuat dalam bentuk lotio dengan konsentrasi 1%, 2%, dan 3% memiliki efektivitas repelan berturut-turut adalah 90%, 96% dan 100%. Dilakukan uji stabilitas fisik terhadap lotio 0%,1%, 2%, dan 3% sebagai berikut; uji organoleptis (warna kecoklatan, aroma khas tembakau dan homogen), uji daya sebar (6,1cm; 6,1cm; 6,2cm; 6,4cm), uji daya lekat (3,06detik; 2,8detik; 2,75detik; 2,61detik), uji cycling test (warna kecoklatan, konsistensi kental, homogen, dan aroma khas tembakau), uji pH (7;7;7;6,3), dan uji viskositas (4.708cPs, 4.575cPs, 4.465cPs, 4.403cPs). Hasil analisis One Way ANOVA menunjukkan adanya perbedaan yang signifikan pada setiap konsentrasi lotio ekstrak daun tembakau dan uji Tukey menunjukkan kemampuan repelan konsentrasi 3% memiliki persen repelansi 100%. Hal ini dapat disimpulkan bahwa lotio ekstrak etanol daun tembakau (Nicotiana tabaccum. L) memiliki efektivitas repelan.Kata kunci: : daun tembakau; ekstrak; lotion; repelan; ANOVA

Synerisis and Cycling Test the Sendok Leaf Extract Gel (Plantago Mayor L.) with Optimization of Variations of Gelling Agent Carbomer 940 Concentration

The purpose of this study was to determine the effect of the concentration of the gelling agent Carbomer 940 on the gel of daun Leaf Extract (Plantago major L.). The method used is the experimental method. The extraction method used is maceration with 96% alcohol. The design in this study is the manufacture of a gel formulation of sendok Leaf extract (Plantago major L.) with the concentration of the gelling agent Carbomer 940 and the syneresis test. Syneresis test results show that all formulas do not occur syneresis. The results of the cycling test showed that the sendok Leaf (Plantago major L.) extract gel was not affected by storage temperature. The conclusion of this study shows the effect of variations in the concentration of the gelling agent Carbomer 940 on the quality of gel.

FORMULATION AND EFFECTIVITY OF THE ANTIOXIDANT GEL PREPARATION CONTAINING ZEAXANTHIN AS ANTI AGING

Objective: This study was conducted to determine the IC50 value of zeaxanthin, to know the formula that meets the requirements of the results of physical evaluation of gel preparations, and to know the effectiveness of zeaxanthin gel as an anti-aging on the skin. Methods: The preparation of this gel is made using zeaxanthin as an active substance with concentrations of 5%; 7.5%; and 10%. Tests conducted are organoleptic test, homogeneity test, pH, spreadability, viscosity, irritation test and cycling test. Tests conducted are organoleptic test, homogeneity test, pH, spreadability, viscosity, irritation test and cycling test. Tests on the effectiveness of zeaxanthin gel preparations against the backs of volunteers' hands were divided into 4 groups as well as testing conducted over 28 d. Results: The results showed a value of IC50 zeaxanthin of 9.044 μg/ml, all gel preparations met the requirements of physical evaluation results except in cycling test and test results of the effectiveness of zeaxanthin gel preparations on the backs of volunteer hands there was an increase in humidity with an average increase of 33.17%±11.867 and wrinkles obedience with an average decrease of 47.466%±7.115. Conclusion: Zeaxanthin can be formulated as gel anti-aging.

Uji Aktivitas Antioksidan Sediaan Masker Hidrogel Ekstrak Etanol Teh Hijau (Camellia sinensis) Dengan Metode BCB

Green tea is a plant that contains antioxidants. The content of green tea compounds that act as antioxidants are polyphenols. The addition of natural antioxidants in hydrogel masks has the potential as an alternative to synthetic antioxidants. The purpose of this study was to determine the antioxidant activity of the hydrogel mask preparation of green tea (Camellia sinensis L.) ethanol extract. The method of determining antioxidant activity was carried out by the -Carotene Bleaching (BCB) method. The stability test of the preparation using the cycling test method was carried out for 6 cycles including organoleptic, viscosity, pH, homogeneity tests. The data obtained from the antioxidant activity test is the IC50 value. The IC50 results of the green tea ethanol extract were 27,162 ppm, while the IC50 values for the formulations 1, 2 and 3 were 40,893 ppm, respectively; 35,348 ppm; and 32,270 ppm. The results of the stability test showed that the preparation was stable from the parameters of viscosity, pH and homogeneity but not stable from the organoleptic parameters because there was a color change in the preparation containing the extract after the cycling test process. In conclusion, both extracts and hydrogel mask preparations contain high antioxidants with relatively stable dosage forms.Keywords: Green Tea, Antioxidants, Hydrogel Mask, -Carotene Bleaching (BCB), IC50. AbstrakTeh hijau merupakan salah satu tanaman yang mengandung antioksidan. Kandungan senyawa teh hijau yang berperan sebagai antioksidan adalah polifenol. Penambahan antioksidan alami pada masker hidrogel berpotensi sebagai alternatif pengganti antioksidan sintetis. Tujuan penelitian ini adalah mengetahui aktivitas antioksidan sediaan masker hidrogel ekstrak etanol teh hijau (Camellia sinensis L.). Metode penentuan aktivitas antioksidan dilakukan dengan metode β-Carotene Bleaching (BCB). Pengujian stabilitas sediaan dengan metode cycling test dilakukan sebanyak 6 siklus meliputi uji organoleptik, viskositas, pH, homogenitas. Data yang diperoleh dari uji aktivitas antioksidan berupa nilai IC50. Hasil IC50 ekstrak etanol teh hijau sebesar 27,162 ppm, sedangkan pada sediaan menunjukan nilai IC50 berturut-turut dari formulasi 1, 2 dan 3 sebesar 40,893 ppm; 35,348 ppm; dan 32,270 ppm. Hasil uji stabilitas menunjukkan sediaan stabil dari parameter viskositas, pH dan homogenitas tetapi tidak stabil dari parameter organoleptik karena terjadi perubahan warna pada sediaan yang mengandung ekstrak setelah proses cycling test. Kesimpulannya ekstrak maupun sediaan masker hidrogel mengandung antioksidan tinggi dengan bentuk sediaan relatif stabil.Kata kunci:Teh Hijau; Antioksidan; Masker Hidrogel; β-Carotene Bleaching (BCB);IC50.

Karakterisasi Dan Optimasi Formula Sediaan Gel Ekstrak Etanol Kulit Buah Pisang Kapas (Musa paradisiaca Linn.)

Cotton banana peel (Musa paradisiaca Linn.) has not been used by the community. Nanoparticles are solid colloidal particles with a diameter of 10-1000 nm. This study aims to make ethanol extract of cotton banana peel (Musa paradisiaca Linn.) as an active substance in the form of nanoparticles formulated in gel preparations and to determine the evaluation of cotton banana peel (Musa paradisiaca Linn.) nanoparticle gel. The method of making nanoparticles of ethanolic extract of cotton banana peel (Musa paradisiaca Linn.) in this research is ionic gelation. Nanoparticles of ethanolic extract of cotton banana peel (Musa paradisiaca Linn.) were characterized using particle size analyzer. Evaluation of gel preparations included organoleptic tests, homogeneity, pH, dispersibility, adhesion, viscosity and cycling tests. The cycling test includes organoleptic, pH and viscosity testing. Cycling test observations were carried out for 6 cycles. Characterization of nanoparticles of ethanolic extract of cotton banana peel (Musa paradisiaca Linn.) had a particle size of 220.3 nm with a polydipsia index of 0.139. Evaluation of pH preparations has a pH of 6, viscosity ranges from 7116 cps – 8095 cps, dispersion ranges from 5.1 cm to 5.4 cm, adhesion ranges from 1.11 seconds to 7.54 seconds. The results of the cycling test showed a change in the color of the preparation, while the cycling test for pH and viscosity did not change the stability. Conclusion The cotton banana peel extract (Musa paradisiaca Linn.) can be made into smaller particles or nanoparticles using the ionic gelation method and the evaluation of the nanoparticle gel preparation of the cotton banana peel (Musa paradisiaca Linn.) extract has met the requirements.Keywords: Cotton banana peel, gel, nanoparticles, evaluation AbstrakKulit buah pisang kapas (Musa paradisiaca Linn.) belum dimanfaatkan oleh masyarakat. Nanopartikel merupakan partikel koloid padatan dengan diameter 10-1000 nm. Penelitian ini bertujuan untuk membuat ekstrak etanol kulit buah pisang kapas (Musa paradisiaca Linn.) sebagai zat aktif dalam bentuk nanopartikel yang diformulasi dalam sediaan gel dan untuk mengetahui evaluasi gel nanopartikel kulit buah pisang kapas (Musa paradisiaca Linn.). Metode pembuatan nanopartikel ekstrak etanol kulit buah pisang kapas (Musa paradisiaca Linn.) pada penelitian ini yaitu gelasi ionik. Nanopartikel ekstrak etanol kulit buah pisang kapas (Musa paradisiaca Linn.) dikarakterisasi menggunakan particle size analyzer. Evaluasi sediaan gel meliputi uji organoleptis, homogenitas, pH, daya sebar, daya lekat, viskositas dan cycling test. Pengujian cycling test meliputi pengujian organoleptis, pH dan viskositas. Pengamatan cycling test dilakukan selama 6 siklus.Karakterisasi nanopartikel ekstrak etanol kulit buah pisang kapas (Musa paradisiaca Linn.) memiliki ukuran partikel 220,3 nm dengan indeks polidipersitas 0,139. Evaluasi sediaan pH memiliki pH 6, viskositas rentang 7116 cps – 8095 cps, daya sebar rentang 5,1 cm -5,4 cm, daya lekat rentang 1,11 detik – 7,54 detik. Hasil pengujian cycling test terdapat perubahan warna dari sediaan, sedangkan pengujian cycling test terhadap pH dan viskositas tidak mengalami perubahan stabilitas. Kesimpulan ekstrak kulit buah pisang kapas (Musa paradisiaca Linn.) dapat dibuat dalam partikel yang lebih kecil atau nanopartikel dengan menggunkan metode gelasi ionik dan evaluasi sediaan gel nanopartikel ekstrak etanil kulit buah pisang kapas (Musa paradisiaca Linn.) telah memenuhi persyaratan.Kata kunci: Kulit buah pisang kapas, gel, nanopartikel, evaluasi