bacterial detection
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PLoS ONE ◽  
2022 ◽  
Vol 17 (1) ◽  
pp. e0262416
Author(s):  
Marie S. Rye ◽  
Kerryn L. Garrett ◽  
Robert A. Holt ◽  
Cameron F. Platell ◽  
Melanie J. McCoy

Background Mucosal infiltration by certain bacterial species may contribute to the development and progression of colorectal cancer (CRC). There is considerable variation in reported detection rates in human CRC samples and the extent to which bacterial infiltration varies across regions of the primary tumour is unknown. This study aimed to determine if there is an optimal site for bacterial detection within CRC tumours. Methods Presence of target bacterial species was assessed by quantitative real-time PCR (qPCR) in 42 human CRC tumours. Abundance in primary tumour regions, normal epithelium and at metastatic sites was investigated in an expanded cohort of 51 patients. Species presence/absence was confirmed by diversity profiling in five patients. Correlation with total bacterial load and clinicopathological features was assessed. Results Fusobacterium nucleatum and Bacteroides fragilis were detected in tumours from 43% and 24% of patients, respectively (17% positive for both species). The optimal detection site was the tumour luminal surface (TLS). Patients testing positive at the TLS frequently tested negative at other sites, including central tumour and invasive margin. F. nucleatum was detected at a higher frequency in tumour versus normal epithelium (p < 0.01) and was associated with more advanced disease (p = 0.01). Detection of both species correlated with total bacterial load. However, corroboration of qPCR results via diversity profiling suggests detection of these species may indicate a specific microbial signature. Conclusions This study supports a role for F. nucleatum in CRC development. Presence of F. nucleatum and B. fragilis varies across primary tumour regions, with the TLS representing the optimal site for bacterial detection.


2021 ◽  
Vol 25 (12) ◽  
pp. 1-8
Author(s):  
Hoorieh Fallah ◽  
Tannaz Asadishad ◽  
Gholam M. Parsanasab ◽  
Sulaiman Wadi Harun ◽  
Waleed S. Mohammed ◽  
...  

Diagnostics ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 46
Author(s):  
Cheng-Han Chen ◽  
Yu-Ting Tsao ◽  
Po-Ting Yeh ◽  
Yu-Hsiang Liao ◽  
Yi-Tzu Lee ◽  
...  

Early detection of microorganisms is essential for the management of infectious diseases. However, this is challenging, as traditional culture methods are labor-intensive and time-consuming. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide-phenazine methosulfate (MTT-PMS) assay has been used to evaluate the metabolic activity in live cells and can thus be used for detecting living microorganisms. With the addition of NaOH and Tris-EDTA, the same approach can be accelerated (within 15 min) and used for the quick detection of common bacterial pathogens. The assay results can be evaluated colorimetrically or semi-quantitatively. Here, the quick detection by MTT-PMS assay was further investigated. The assay had a detection limit of approximately 104 CFU/mL. In clinical evaluations, we used the MTT-PMS assay to detect clinical samples and bacteriuria (>105 CFU/mL). The negative predictive value of the MTT-PMS assay for determining bacteriuria was 79.59% but was 100% when the interference of abnormal blood was excluded. Thus, the MTT-PMS assay might be a potential “rule-out” tool for bacterial detection in clinical samples, at a cost of approximately USD 1 per test. Owing to its low cost, rapid results, and easy-to-use characteristics, the MTT-PMS assay may be a potential tool for microorganism detection.


2021 ◽  
Vol 8 (1) ◽  
pp. 23-33
Author(s):  
Barbora Vidová ◽  
Andrej Godány ◽  
Ernest Šturdík

In this article are reviewed the promising uses of phage display in the areas such as microbial pathogens detection of and vaccination. Phage display is a molecular technique by which foreign proteins are expressed at the surface of phage particles. Such phages thereby become vehicles for expression that not only carry within them the nucleotide sequence encoding expressed proteins, but have also the capability to replicate. Recent data acquired from genome sequencing and advances in phage biology research have aided the development of phage-derived bacterial detection and treatment strategies.


2021 ◽  
pp. 2107574
Author(s):  
Wei Zhao ◽  
Meng Ding ◽  
Xu Zhang ◽  
Zhirong Xin ◽  
Lingjie Song ◽  
...  
Keyword(s):  

2021 ◽  
pp. 2101546
Author(s):  
Ashna Gopal ◽  
Li Yan ◽  
Saima Kashif ◽  
Tasnim Munshi ◽  
Roy AL Vellaisamy ◽  
...  

Author(s):  
Babeker Awad M ◽  
Abdalla AA ◽  
Ahmed AR ◽  
Ebrahiem Mohammed Alhadi

This study was designed to determine the microbial and heavy metal contamination level in expeller’s groundnut oil. Ten samples of groundnut oil were collected, labeled from E1 to E10 and the microbial and heavy metals examination tests were laboratory assessed. The study results showed that, the Total Count of Bacteria (TCB) for all oil Expellers samples was 33.8 Colony Forming Unit (Cfu)/ml oil. The highestt value was (111 Cfu/ml) in E10 and the lowest value (7 Cfu/ml oil) in E1. Concerning the Total Count of Fungal (TCF) for all samples was 31.4 and 6.75 Cfu/1ml for the yeast and mould respectively. Yeasts highest count was 109 Cfu/ml in E10 and the lowest count was 6 Cfu/ml in E1. while moulds highest count was 18 Cfu/ml in E10 and the lowest number of 2 Cfu/ml oil was found in E2. The bacterial detection test of Staph, Bacillus, Coliform, and E.Coli in the oils Expellers explored that no bacterial presence in E1, E3, and E7 but, the four types were recorded in E5, E9 and E10. On the other hand E2, E6 and E8 showed positive detection to Staph and Bacillus, while E4 was positive to Bacillus detection only. The Concentration of Iron (Fe) on expeller oil samples (E2, E3, E4, E6, E7, E8, E9 and E10) was higher than the minimum specification limit of 5meq/kg for heavy metals, but E1 and E5 were within the minimum specification limit. All expellers oil samples were higher than acceptable limit for Copper (Cu) concentration that established by SSMO and Codex alimentary for heavy metals of less than 0.1meq/kg. As well as, the Concentration value of Lead (Pb) all expeller oil samples were above the specification limit of heavy metals that should not be more than 0.1meq/kg.


2021 ◽  
Author(s):  
Naomi Rich ◽  
Jourdan Brune ◽  
David Duclos
Keyword(s):  

Author(s):  
Maria Mathisen ◽  
Sudha Basnet ◽  
Andreas Christensen ◽  
Arun K. Sharma ◽  
Garth Tylden ◽  
...  

Respiratory viruses are an important cause of respiratory tract infections in children but are underrecognized as a cause of pneumonia hospitalization in low-income settings. Previously, we detected at least one of seven respiratory viruses by PCR in 30% of young Nepalese children hospitalized with severe pneumonia over a period of 36 months.


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