Phylogenetic and sequencing analysis of Chikungunya virus (CHIKV) strains from local Pakistani population

Objective: The present study aims to outline the genotype of circulating CHIKV strains in Pakistan and their phylogenetic analysis Methods: The study is being conducted at Armed Forces Institute of Pathology after the approval by Institutional Review Board. The study was conducted during the period of Aug 2016 to Sep 2017. During this period we have got the total 11 suspected Chikungunya samples in our center and out of these 7 were positive. The viral RNA was extracted, amplified and then the purified product was sequenced by using Sanger dideoxy method. Structural Prediction and Analysis was performed by using Phyre2 and then models were visualized in PyMol2.2. Results: In our study total 11 samples were analyzed, in which 7 samples were positive and the amplified product from five samples were later sequenced because these samples were of good quality for sequencing. Phylogenetic tree was constructed. Five sequences generated in our study were aligned with other CHIKV sequences reported from all over the world. Conclusion: The phylogenetic analysis showed that the strains present in Pakistan belong to the ECSA genotype and derived from India and South East Asia. These results help in the better understanding of CHIKV evolution in the world and discovered that how CHIKV originated in Pakistan. Keywords: Chikungunya; glycoprotein E1; glycoprotein E2; Aedes aegypti. Continuous...

Relationships between test anxiety, self-regulation and strategies for coping with stress, in professional examination candidates

El objetivo de la investigación fue establecer relaciones de asociación, interdependencia y predicción estructural entre las variables ansiedad evaluativa, authorregulación y estrategias de afrontamiento del estrés. El marco teórico de referencia fue el modelo de la competencia de Studying, Learning, and Performing under Stress (SLPS). Participaron 142 estudiantes, que se estaban preparando en academias de Almería (España) para obtener plaza como maestros en centros públicos. Para la recogida de datos se administraron cuestionarios escritos previamente validados. El diseño fue ex post-facto lineal, con análisis de asociación bivariada, inferenciales (ANOVAs y MANOVAs) y de predicción estructural. Los resultados mostraron una relación negativa entre la ansiedad evaluativa y la autorregulación, especialmente en los estudiantes con alta emocionalidad, con un impacto negativo para la toma de decisiones. También se encontraron relaciones positivas entre la ansiedad evaluativa y las estrategias de afrontamiento del estrés. Por último, se constató la relación de predicción positiva entre la autorregulación y las estrategias de afrontamiento, a la vez que los análisis asociativos e inferenciales destacaron el papel de las metas como determinantes de las estrategias usadas para afrontar el estrés, especialmente, las referidas a la focalización en la resolución de problemas. Se discuten los resultados y se establecen implicaciones para las mejoras de estos procesos en los estudiantes opositores. The research objective was to establish relationships of association, interdependence and structural prediction between the variables of test anxiety, self-regulation and stress coping strategies. The theoretical framework of reference was the Studying, Learning and Performing under Stress (SLPS) Competency Model. Participating were 142 students who were preparing for professional examinations to attain a post as public school teacher (primary education), enrolled at academies in Almería (Spain) for this purpose. Previously validated questionnaires were administered for data collection. The study design was linear ex post-facto, with bivariate, inferential analyses of association (ANOVAs and MANOVAs) and of structural prediction. Results showed a negative relationship between test anxiety self-regulation, especially in students with high emotionality, and a negative impact on decision making. Positive relationships were found between test anxiety and strategies for coping with stress. Finally, a positive predictive relationship was verified between self-regulation and coping strategies, while associative and inferential analyses highlighted the role of goals as determining factors in strategies used for coping with stress, especially strategies that focus on problem solving. Results are discussed and implications for improving these processes in professional examination candidates are established.

Structural Prediction Model Satisfaction and Buying Decision of Muslim Consumers Towards Fast Food by Halal Labeling

This exploration means to comprehend what impacts Muslims in Bandung in creation choices on purchasing inexpensive food. The objects of this exploration are Muslim cheap food customers in Bandung. Information was gathered through an overview utilizing polls as a helping instrument. There were 180 respondents as tests. The information examination was led utilizing Structural Equation Modeling – Partial Least Square (SEM–PLS) approach. The exploration results show that the halal logo has a positive and critical impact on purchasing choices and that the clients' fulfillment likewise has a positive and huge effect on purchasing choices. Moreover, buyers' fulfillment can intervene in the impact of the halal logo on purchasing choices. This examination result is relied upon to urge eatery supervisors to be more mindful of the significance of the halal logo on their items.