cry gene
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2022 ◽  
Author(s):  
Chaochen Huang ◽  
Pengbo Li ◽  
Junfeng Cao ◽  
Zishou Zheng ◽  
Jinquan Huang ◽  
...  

Abstract Background: The cryptochromes (CRY) comprise a specific blue light receptor for plants and animals, which play crucial roles in physiological processes of plant growth, development, and stress tolerance. Results: In the present work, a systematical analysis of CRY gene family from five allotetraploid cotton species, G. hirsutum, G. barbadense, G. tomentosum, G. mustelinum and G. darwinii together with seven diploid species. There were 18, 17, 17, 17, and 17 CRYs identified in G. hirsutum, G. barbadense, G. tomentosum, G. mustelinum and G. darwinii, respectively, whereas five to nine CRY genes were identified in the diploid species. Phylogenetic analysis of the protein-coding sequences revealed that CRY genes from the allotetraploids G. hirsutum and G. barbadense, three diploid cotton species (G. raimondii, G. herbaceum, and G. arboreum), and Arabidopsis thaliana could be classified into seven clades. Synteny analysis suggested that the homoeolog of G. hirsutum Gh_A02G0384 has undergone an evolutionary loss event in the other four allotetraploid cotton species. Cis-element analysis predicated the possible functions of CRY genes in G. hirsutum. Public RNA-seq data were investigated to analyze the expression patterns of G. hirsutum CRY genes in various tissues as well as gene expressions under abiotic stress treatments. Conclusion: These results indicated the possible functions of G. hirsutum CRY genes in differential tissues as well as in response to abiotic stress during the cotton plants life cycle.


2021 ◽  
Author(s):  
Drishtant Singh ◽  
Samiksha . ◽  
Seema Madhumal Thayil ◽  
Satwinder Kaur Sohal ◽  
Anup Kumar Kesavan

Abstract An expression system based on the cry gene regulatory elements was constructed. The Terminator region of cry gene from B. thuringiensis subsp. kurstaki HD-1 was cloned in pSG1151 plasmid downstream to gfpmut1. The promoter region of the cry gene was amplified to give three different reading frames. The Promoter region of cry gene was cloned in pSG1151T plasmid upstream to gfpmut1. The expression of GFP under the promoter/terminator expression system was evaluated by checking the expression of gfpmut1 under the same promoter. The GFP content of pSG1151 and three constructs; pDSA1, pDSA2 and pDSA3 were compared by fluorescence spectroscopy. The fluorescent intensity of pSG1151 and pDSA1 were compared at time interval of 6 hours upto 72 hours. Both the samples showed detectable fluorescence that increased with time up to 12 hours, but the increase in the fluorescence of pDSA1 was 3 times higher as compared to pSG1151. A cold peptidase gene was cloned under the control of the cry promoter. The transformed E.coli DH5α colonies were patched on skim milk agar plates and the clones of pSG1151CP and pDSA1CP were compared on the basis of zone of clearance. The zone of clearance of pDSA1CP was much higher as compared to that of pSG1151CP. The cell-free supernatant of Bacillus sp. S1DI 10 and recombinant pDSA1CP collected at different time points was assayed for the specific activity of the extracellular protease. At 72 hours the protease activity in pDSA1CP was 2.7 fold higher compared to that of wild Bacillus sp. S1DI 10.


2021 ◽  
Vol 74 ◽  
pp. 106497
Author(s):  
Zhao Shu-Qin ◽  
Zhang Yong ◽  
Gao Yuan ◽  
Yang Xiao-Pu ◽  
Yang Zhen ◽  
...  

2019 ◽  
Vol 113 (4) ◽  
pp. 740-754
Author(s):  
Ruibin Zhang ◽  
Leyla Slamti ◽  
Lei Tong ◽  
Emilie Verplaetse ◽  
Lixia Ma ◽  
...  

2018 ◽  
Vol 17 (1) ◽  
Author(s):  
Chihang Cheng ◽  
Jialing Qin ◽  
Choufei Wu ◽  
Mengying Lei ◽  
Yongjun Wang ◽  
...  

2018 ◽  
Vol 9 ◽  
Author(s):  
Kavita Nair ◽  
Roda Al-Thani ◽  
Dhabia Al-Thani ◽  
Fatima Al-Yafei ◽  
Talaat Ahmed ◽  
...  

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