Evaluation of The Antifungal Activity of Nasturtium officinale (watercress) Oil with Calcium Hydroxide against Candida Albicans Isolated from Root Canal

Background: The interest in herbal extracts as antimicrobial agents has increased over the past few years in endodontic therapy. Nasturtium officinale (watercress) is a promising plant with great medicinal values. This study aimed to investigate the antifungal activity of watercress oil in combination with calcium hydroxide against Candida albicans as intracanal medicament. Materials and Methods: Candida albicans was isolated from patients with necrotic root canal or failed root canal treatment. The sensitivity of Candida albicans to different concentrations of watercress oil extract was determined by using the agar well diffusion method in comparison with calcium hydroxide paste. The agar plate method was used to determine the minimum fungicidal concentration (MFC) of the tested oil against the fungus. The combination of the oil extract of Nasturtium officinale with calcium hydroxide was evaluated and compared to calcium hydroxide paste with iodoform by using the agar well diffusion method. Results: The oil extract exhibited antifungal activity against Candida albicans, this activity was found to be increased as the concentration of extract increased. The tested combination of watercress oil extract with calcium hydroxide revealed larger inhibition zones than the ones formed by each tested agent individually. Conclusion: The oil extract of Nasturtium officinale is active against Candida albicans suggesting its potential to be used as an intracanal medicament alone or in combination with calcium hydroxide.

Pupuk Organik Cair Limbah Ikan (Pocli) dan Produksi Tanaman Selada (Nasturtium Officinale R. Br)

Pandemi Covid-19 mengggu kgiatan masyarakat. Tujuan penelitian untuk meningkatkan pertumbuhan dan produksi tanaman selada di pekarangan rumah masarakat desa.Tempat Penelitian di Desa Nusajaya Halmahera Timur Indonesia. selama Januari – Mei 2021. Perlakuan tanpa pupuk orgnik cair limbah ikan ((POCLI)) 1 liter air/3 polybag (A1)., (POCLI) 5 cc/1 liter air/3 polybag (A2)., (POCLI) 10 cc/1 liter air/3 polybag (A3)., (POCLI) 15 cc/1 liter air/3 polybag (A4)., (POCLI) 20 cc/1 liter air/3 polybag (A5)., (POCLI) 25 cc/1 liter air/3 polybag (A6) Masing- masing polybag diberikan 333 cc. Parameter agronomi meliputi: 1) Tinggi selada (cm), 2) banyak daun selada, 3) Panjang (cm), 4) Bobot segar sampel (g) dengan analisis ragam (Anova). Kesempulan. Hasil analisis ragam menunjukan bahwa perlakuan POCLI 25 cc/L air/3 polybag (A6) berpengaruh terhadap seluruh parameter pengamatan selada, umur 14 HST tinggi 8 cm, umur 21 HST tertinggi 13 cm dan pada umur 28 HST tinggi 20 cm. Pada saat panen menhasilkan jumlah daun selada terbayak 10 helai, daun terpanjang 20 cm, dan bobot sampel terberat 135 g bila dibandingkan dengan perlakuan lainnya. Usur makro dan mikro didalam POCLI sehingga mendorong, mempercepat pertambahan ukuran, panjang selada.

Evaluation of the efficiency of an integrated biofilm and phytoremediation system with Nasturtium officinale for the treatment of municipal wastewater in Huancavelica

In this research removal efficiency was evaluated of organic pollutants of a biofilm and phytoremediation integrated system for municipal wastewater treatment in Huancavelica. A pilot plant consisting of a settler was installed that also retained oils and fats, a biological filter using as support material the calcareous stuff and a phytoremediation process with Nasturtium officinale (watercress) The hydraulic retention time was 5.5 hours and the flowrate of 0.011 L s -1, the evaluation was made of the proper functioning of processes by monitoring control parameters. The pollutant removal efficiency was determined by Student's "t" test (p <0.05). An average pH value of 7.57 and a temperature of 12.07 ° C were obtained, subsequently the measurement of BOD5 was initiated, obtaining maximum effluent values of 131 ppm and minimum values of 45 ppm and a removal efficiency of 44.8% was obtained, for COD. In total coliforms, fecal coliforms and Escherichia coli the removal was 66.67%, 94.59%, 81.22% and 72.12% respectively, oil and fat analysis was also performed and total suspended solids, however high removal efficiency was not obtained. It is concluded that the parameters of BOD5 and COD comply with the MPL for the effluents of municipal domestic wastewater treatment plants, while the rest of the parameters analyzed do not comply with the regulations. however high removal efficiency was not obtained. It is concluded that the parameters of BOD5 and COD comply with the MPL for the effluents of municipal domestic wastewater treatment plants, while the rest of the parameters analyzed do not comply with the regulations. however high removal efficiency was not obtained. It is concluded that the parameters of BOD5 and COD comply with the MPL for the effluents of municipal domestic wastewater treatment plants, while the rest of the parameters analyzed do not comply with the regulations.

A Comparative Study of Antioxidant Potential and Phytochemical Contents of different Extracts of Wild Nasturtium Officinale W T Aiton Collected from Kumaun Region of Uttarakhand

Nasturtium officinale W. T Aiton or “watercress” (Brassicaceae) is a nutritionally valuable plant that is the richest source of carotenoids, polyphenols, iron, calcium, iodine, folic acid, essential vitamins and minerals. It is traditionally used for their appetiser, anti-scorbutic, stimulant, diuretic and detoxifying properties. The present study was conducted to investigate the antioxidant potential, phytochemical contents (total phenolic, flavonoids and tannin contents) as well as a preliminary phytochemical screening of different extract of aerial parts of Nasturtium officinale collected from the Kumaun region. The different extracts showed significant antioxidant activity as well as total phenolic, flavonoids and tannin contents. The preliminary phytochemical screening showed the presence of several phytochemical constituents such as carbohydrates, proteins, amino acids, glycosides, alkaloids and others. The hydroalcoholic extract possess significantly (P<0.05) higher antioxidant potential with IC50 value (0.333 mg/ml, 0.509mg/ml)and EC50 value (3.537 mg/ml) by 2,2-diphenyl-1-picrylhydrazyl(DPPH), 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid)(ABTS), and ferric-reducing antioxidant power (FRAP) methods, respectively, as well as highest total tannin, flavonoid and phenolic contents among all the extracts. These results provide substantial evidence that the plant Nasturtium officinalehas antioxidant potential and valuable sources of phytochemicals.