Correction of the organoleptic characteristics of milk with a protein ingredient in the development of a product for herodietic nutrition

В статье представлены результаты исследований, направленных на корректировку органолептических характеристик молока с сухим гидролизатом говяжьего белка при разработке продукта для геродиетического питания. Описаны подходы, направленные на достижение поставленной цели. Подобрано оптимальное количество вносимого гидролизата говяжьего белка, и показан сравнительный график аминокислотных скоров молока и смеси молока с белковым ингредиентом, указывающий на целесообразность его использования. Приведен перечень технологических операций, применяемых при выработке опытных образцов продукта. Представлены результаты подбора корректирующих компонентов и параметры технологических операций, применяемых для выработки опытных образцов. Приведены данные органолептической оценки опытных образцов, и сделан вывод о наиболее приемлемых корректирующих компонентах применительно к сочетанию молока и гидролизата говяжьего белка. The article presents the results of research aimed at correcting the organoleptic characteristics of milk with dry beef protein hydrolysate in the development of a product for herodietic nutrition. Approaches aimed at achieving this goal are described. The optimal amount of beef protein hydrolysate is selected and a comparative graph of the amino acid pressure of milk and a mixture of milk with a protein ingredient is shown, indicating the feasibility of its use. The list of technological operations used in the development of product prototypes is given. The results of the selection of corrective components and the parameters of technological operations used for the development of prototypes are presented. The data of the organoleptic evaluation of the experimental samples are presented and the conclusion is made about the most acceptable corrective components in relation to the combination of milk and beef protein hydrolysate.

PSVI-25 Inclusion of Lemna as a plant-based protein ingredient in dog and cat diets

Our objective was to evaluate the inclusion of a novel plant-based protein (Lemna; MC Select; Parabel®; Vero Beach, FL) in dog diets at 0, 5, and 10% and cat diets at 0, 10 and 15% for palatability, stool quality, and nutrient digestibility. We hypothesized that Lemna would be a viable protein source in both cat and dog diets by showing no detriments to nutrition outcomes. All feeding tests were conducted at an independent research facility (Susquehanna, PA). A standard 2 bowl palatability test over a 2-day period was done with adult dogs and cats (n = 30 each) to determine intake ratios (IR) between test diets (Lemna -containing diets) and control (0% Lemna) diet. Total tract nutrient digestibility was conducted with 18 adult dogs and 21 adult cats (n=6–7 per diet) with 5 days of diet acclimation followed by 5 days of total fecal collection. Stool quality was evaluated on a 1–5 scale where 1= non-formed/diarrhea and 5= hard, formed. Palatability data was analyzed via Wilcoxon Signed Rank, and digestibility and stool quality data were analyzed by ANOVA with a Tukey’s post-hoc means separation (SAS version 9.4). Intake ratios in cats between 10% Lemna and control were significantly (P < 0.05) in favor of control, while no difference was observed between 15% Lemna and control. For dogs, 5% and 10% Lemna had significantly (P < 0.05) lower IR demonstrating a preference to control. Both cats and dogs fed Lemna diets had acceptable stool quality (3.42 avg for cat and 3.34 avg for dog). No detriments in nutrient digestibility were observed in dogs fed 5% and 10% Lemna; however, cats fed 10% and 15% Lemna had significantly (P < 0.05) lower dry matter, protein, and energy digestibility versus control. In conclusion, these data suggest more development is needed for Lemna inclusion in companion animal diets. Thursday, July 23, 2020

Protein Ingredient in Saliva on Oral Dryness Patients Caused by Calcium Blocker

Oral dryness as a side effect of certain drugs is increasing. The aim of this study was to examine the change of the protein ingredient in saliva of oral dryness patients caused by calcium blocker. Six patients taking calcium blocker and six healthy elderly were enrolled. Unstimulated salivary flow rate, protein concentration, and flow rate of protein were measured and compared between the patients taking calcium blocker and healthy elderly. iTRAQ (Isobaric Tag for Relative and Absolute Quantitation) proteomic analysis was performed to extract the salivary protein changed in patient taking calcium blocker, and the intensities of Western blotting products were quantified (unpaired t-test). Unstimulated salivary flow rate was significantly lower on patients taking calcium blocker (p < 0.01). Protein concentration tended to be higher and the flow rate of protein tended to be lower on patients. As the result of iTRAQ proteomic analysis, calmodulin-like protein 3, glutathione S-transferase P, and keratin type I cytoskeletal 13 increased characteristically in patient taking calcium blocker, and the expression in calmodulin-like protein 3 was significantly larger (p < 0.01). The results of this study indicated that calmodulin-like protein 3 increased in patients taking calcium blocker and could be a salivary biomarker for oral dryness caused by calcium blocker.