Assessment of the influence of the mineral complex of rutin on the degree of expression of anti-diabetic activity

Introduction. Type 2 diabetes mellitus is currently considered one of the most common non-communicable diseases. For the prevention and concomitant treatment of this pathology, various herbal remedies are successfully used, such as, for example, blueberry shoots. The plant contains phenolic compounds (anthocyanins, flavonoids, phenolcarboxylic and organic acids), which have antioxidant and hypoglycemic effects, and also accumulates macro- and microelements (Ca, Mg, Zn, Mn), which in turn can affect the course of diabetes mellitus. Complexes of elements with phenolic biological active substances (BAS) can affect the formation of a pharmacological response or change its severity. Therefore, it is possible to put forward a hypothesis about the potentiation of the antidiabetic action of phenolic compounds when they exist in the form of mineral complexes.Aim. To carry out a comparative assessment of the antidiabetic activity of the mineral complex rutin with zinc in comparison with precursor substances and extraction from blueberry shoots to predict the effect of elements on the course of this pathology.Materials and methods. The objects of the study were an aqueous solution (C = 0.18 mg/ml) of a model complex of rutin with zinc with a molar ratio of components of 1 : 1 and blueberry shoots purchased from a pharmacy in St. Petersburg. According to the information on the packaging, the region of raw material procurement is Altai Territory, Barnaul, the period for harvesting blueberries is July 2020. The complex of rutin with zinc was obtained according to the method described in the literature from the pharmaceutical substance rutin (Rutin, batch 332, valid until 26.03.2023, Sichuan Guangsong Pharmaceutical Co., Ltd., China, FS 000569-060514) and an aqueous solution (С = 0.13 mg/ml) zinc chloride (Zinc chloride, batch 39/G 4, valid until 09.10.2021, Neva Reaktiv, Russia, STP TU COMP 1-533-2012). The optimal ratio of components 1 : 1 for the formation of a mineral complex was established by us earlier experimentally using the Job's method. The mass of zinc chloride, which must be added to the extraction, and the mass of the complex for the preparation of its aqueous solution were calculated on the basis of the quantitative content of biologically active substances in blueberry shoots and the molar ratio of the components involved in the formation of the complex compound determined by the spectral method. The quantitative content of the main groups of biologically active substances (flavonoids, hydroxycinnamic acids, organic acids) was determined spectrophotometrically on SF-2000 instrument (Russia) and titrimetrically using the methods presented in Russian Pharmacopoeia XIV FS.2.5.0093.18 and FS.2.5.0012.15. The antidiabetic effect of the complex of rutin with zinc was evaluated in comparison with an aqueous extract from the shoots of common blueberries (the ratio of raw materials: extractant – 1 : 80), an aqueous solution of zinc chloride (concentration – 0.36 mg/ml) and their mixture (ratio 1 : 1) on a model of dexamethasone-induced type 2 diabetes mellitus in laboratory animals. Determination of blood glucose concentration was carried out using a portable glucometer "AccuChek Active" (Roche Diabetes Care, Germany). The presence of glucosuria and ketonuria was established using Ketoglyuk-1 test strips (Biosensor AN, Russia). Statistical processing of the results was carried out using the Microsoft Excel program according to OFS.1.1.0013.15.Results and discussion. The results of the content of glucose and ketones in urine showed that in the process of modeling diabetes mellitus, glucosuria and ketonuria are characteristic for all groups of animals, which confirmed the formation of a pathological process in rats. A lower content of glucose and ketones in urine was found in a group of animals that received a complex of rutin with zinc (glucose concentration – less than 2.3 mmol/l, ketone concentration – less than 0.2 mmol/l). Measurement of the concentration of glucose in the blood showed that the complex of rutin with zinc has the most pronounced hypoglycemic effect, while the water extract from blueberry shoots and an aqueous solution of zinc chloride have a minimal antidiabetic effect (blood glucose is 6.9 mmol/l versus 8,1 mmol/l and 7.9 mmol/l, respectively).Conclusion. The study of the influence of elements in the composition of phenolic complexes on the course of diabetes mellitus showed that the introduction of minerals has a positive effect on the severity of the pharmacological effect, which can serve as confirmation of the hypothesis about the potentiation of the antidiabetic effect of phenolic compounds when they exist in the form of mineral complexes. Thus, the complex of rutin with zinc showed the maximum activity in comparison with the solution of zinc chloride, extraction from blueberry shoots and their mixture, which suggests that the form of existence of natural phenolic compounds in the composition of mineral complexes is optimal both from the point of view of accumulation and from the side manifestations of a pharmacological response. The results of the study create the preconditions for further study of the effect of various elements in combination with marker phenolic components of antidiabetic herbal preparations on the course of diabetes mellitus, and also make it possible to conclude that natural mineral complexes have a prophylactic effect against this pathology.

Composite Adsorption of Copper Ions by Iron Oxide, Organic Matter and Clay Mineral Complexes in Sediments: Antagonistic Effect of Organic Matter

The combination of organic matter, iron oxide, and clay minerals is of great significance for the adsorption of copper ions (Cu). The purpose of this study is to explore the characteristics of Cu adsorption and laws governing Cu complexation to organic–inorganic, organic–clay mineral, and iron-oxide–clay mineral complexes in the sediments in the estuary of plateau fault and sinking lake——Dianchi Lake. In this study, Cu adsorption tests were performed on the three complexes, in order to study the kinetic behavior of adsorption, Langmuir and Freundlich isotherm models were used. The samples before and after adsorption were characterized via scanning electron microscope (SEM), Fourier infrared spectroscopy (FTIR), and X-ray diffraction (XRD). Our results show that, the Freundlich isotherm models model was able to describe adsorbent behavior in comparison to the Langmuir models. During the Cu adsorption process, the iron-oxide–clay mineral complex is able to adsorb Cu, via coordination exchange, through the –OH contained therein. Organic-matter–clay mineral complexes bonded to the surfaces of clay minerals by replacing the hydroxyl groups with functional groups (carboxyl groups or phenolic hydroxyl groups) contained in the organic matter. Organic–inorganic composites then adsorbed Cu through the coordination exchange of –OH in the polar functional groups of alcohols, phenols, and carboxylic acids. The adsorption capacity of Cu in these three sediment complexes was observed to have the following order: organic–inorganic complex > organic-matter–clay mineral complex > iron-oxide–clay mineral complex. The semi-quantitative analysis results of Fourier Infrared Spectroscopy show that the organic matter (changes in the peak area of functional groups such as carboxyl groups) in the organic-inorganic composite material has an important effect on the amount of copper ions adsorbed by clay minerals.

Influence of skimming of calves with skim milk substitute on physiological condition and productivity

The high demand for protein during this period of the calf's life is due to the active growth of muscle tissue, and protein is the structural material of all organs. A lack of protein in the diet of calves contributes to a delay in their growth and an excess to the expenditure of additional energy for the deamination of excess amino acids and the elimination of the corresponding decay products through the excretory system of the body. The younger the calves, the higher the protein level in their diet should be. The work aimed to establish the most effective protein norms in the composition of skim milk substitutes for calves over 65 days of age. The study of the influence of feeding skims milk substitute on calves' physiological state and productivity was carried out on four groups of bulls. All tested skim milk replacers varied in protein content but were almost the same in all nutritional parameters. The main ingredients of skim milk substitutes (ZOM 1) for calves of group I were, %: milk proteins – 70, vegetable proteins (soy + wheat) – 29, vitamin and mineral complex, probiotic culture – 1. For calves of group II (ZOM 2) used,%: milk proteins – 70, vegetable proteins (soy + wheat protein) – 29, vitamin-mineral complex – 1. protein) – 29, vitamin and mineral complex – 1. Studies have shown that skim milk substitutes in calves feeding, containing 20 and 22 % protein in the composition of KR-2 compound feed 10 % by weight, was reflected in the improvement of the morpho-biochemical design of the blood. At the same time, there is a tendency to an increase in the concentration of total protein in the blood serum by 3.1 and 3.3 % with a decrease in the amount of urea by 3.5 and 5.2 %, which made it possible to increase the average daily gain in live weight to 3.1 % while reducing costs feed and its price by 1.5 and 0.9 percent.

Disease-Modifying Adjunctive Therapy (DMAT) in Osteoarthritis—The Biological Effects of a Multi-Mineral Complex, LithoLexal® Joint—A Review

Modern advances in molecular medicine have led to the reframing of osteoarthritis as a metabolically active, inflammatory disorder with local and systemic contributing factors. According to the ‘inflammatory theory’ of osteoarthritis, immune response to an initial damage is the key trigger that leads to progressive joint destruction. Several intertwined pathways are known to induce and govern articular inflammation, cartilage matrix degradation, and subchondral bone changes. Effective treatments capable of halting or delaying the progression of osteoarthritis remain elusive. As a result, supplements such as glucosamine and chondroitin sulphate are commonly used despite the lack of scientific consensus. A novel option for adjunctive therapy of osteoarthritis is LithoLexal® Joint, a marine-derived, mineral-rich extract, that exhibited significant efficacy in clinical trials. LithoLexal® has a lattice microstructure containing a combination of bioactive rare minerals. Mechanistic research suggests that this novel treatment possesses various potential disease-modifying properties, such as suppression of nuclear factor kappa-B, interleukin 1β, tumor necrosis factor α, and cyclooxygenase-2. Accordingly, LithoLexal® Joint can be considered a disease-modifying adjunctive therapy (DMAT). LithoLexal® Joint monotherapy in patients with knee osteoarthritis has significantly improved symptoms and walking ability with higher efficacy than glucosamine. Preliminary evidence also suggests that LithoLexal® Joint may allow clinicians to reduce the dose of nonsteroidal anti-inflammatory drugs in osteoarthritic patients by up to 50%. In conclusion, the multi-mineral complex, LithoLexal® Joint, appears to be a promising candidate for DMAT of osteoarthritis, which may narrow the existing gap in clinical practice.

Disease-Modifying Adjunctive Therapy (DMAT) in Osteoarthritis – The Biological Effects of a Multi-mineral Complex, LithoLexal®

Modern advances in molecular medicine have led to reframing osteoarthritis as a metabolically active, inflammatory disorder with local and systemic contributing factors. According to the ‘inflammatory theory’ of osteoarthritis, immune response to an initial damage is the key trigger that leads to progressive joint destruction. Several intertwined pathways are known to induce and govern articular inflammation, cartilage matrix degradation, and subchondral bone changes. Effective treatments capable of halting or delaying the progression of osteoarthritis remain elusive. As a result, supplements such as glucosamine and chondroitin sulphate are commonly used despite the lack of scientific consensus. A novel option for adjunctive therapy of osteoarthritis is LithoLexal®, a marine-derived, mineral-rich extract, that exhibited significant efficacy in clinical trials. LithoLexal® has a lattice microstructure containing a combination of bioactive rare minerals. Mechanistic research suggests that this novel treatment possesses various potential disease-modifying properties, such as suppression of nuclear factor kappa-B, interleukin 1β, tumour necrosis factor α, and cyclooxygenase-2. Accordingly, LithoLexal® can be considered a disease-modifying adjunctive therapy (DMAT). LithoLexal® monotherapy in patients with knee osteoarthritis has significantly improved symptoms and walking ability with higher efficacy than glucosamine. Preliminary evidence also suggests that LithoLexal® may allow clinicians to reduce the dose of nonsteroidal anti-inflammatory drugs in osteoarthritic patients by up to 50%. In conclusion, the multi-mineral complex, LithoLexal®, appears to be a promising candidate for DMAT of osteoarthritis, which may narrow the existing gap in clinical practice.

REFERENCE POINT OF GEOLOGICAL TIME

In the earth sciences, there is a persistent contradiction between modern ideas about the age of the planet and the concept of V.I. Vernadsky about the geological eternity of life. According to Vernadsky, the absolute age of the rock indicates only the time of its last metamorphism, but not the age of the Earth. It is no coincidence that the pregeological substance of a cosmic formation of the planet has not been found. Such substance does not exist because any mineral complex and rock begins to form in the hypergenesis zone. Vernadsky argued that geological history was equal in duration to the existence of the biosphere and its duration was measured by biological time. His concept is confirmed by current biogeochemistry. The history of the biosphere practically coincided with the "canonical" age of the Earth. In a cosmological sense, the picture of the universe cannot be built without the concept of the geological eternity of the biosphere.

Effects of liquid organic mineral complex (MultiMix®) on milk yield, composition and cheesemaking capacity of milk in dairy cows

The objective of this experiment was to investigate the effects of liquid organic mineral complex (MultiMix®) on the milk yield, composition and cheesemaking capacity of milk in dairy cows. Thirty Holstein cows (8 primiparous and 22 multiparous) were used in a randomized complete block design experiment with 15 cows per treatment. Feeding was ad libitum targeting 5% refusals. Milk yield data and samples for fat, true protein, solids not fat (SNF), lactose content and parameters characterizing the milk’s coagulation properties were collected throughout the experiment. Data suggested that MultiMix® administered through the cows’ drinking water had a positive effect on the milk productivity with a high degree of significance (p<0.001). A positive effect on the indicators characterizing the quality composition of milk has also been observed (p<0.001). Additionally, the cheesemaking capacity of milk was enhanced by supplementing animal water with MultiMix® (p<0.001). Overall, the new liquid organic mineral complex used in the present experiment showed promising results for improvement of milk production and composition in dairy cows but further studies are needed to unveil the physiologic mechanisms underlying these beneficial effects.

Yield and quality of carrots when using the Gumiton organo-mineral complex in conditions of radioactive contamination

The aim of this work is to assess the impact of Gumiton peat-based organo-mineral complex on the productivity and quality of carrots and the transition of 137Cs from soil to root crops under conditions of radioactive contamination. According to the results of field experiments on gray forest and soddy-podzolic soils of the Kaluga region of Russia, it is shown that leaf treatment with Gumiton of carrot plantings in different phases of crop development increases the yield of root crops by 20-40%, depending on the variety, soil type and agricultural background. Analysis of studies in the growing experiment on soddy-podzolic soil showed that Gumiton treatment increases the yield of carrots by 30%. The rate of decrease of 137Cs content is 1,2-1,25 times. Gumiton is a highly effective preparation for carrots, which increases the productivity and quality of the crop and helps to reduce the intake of 137Cs in root crops in radioactively contaminated areas.