dietary nitrogen
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Insects ◽  
2022 ◽  
Vol 13 (1) ◽  
pp. 84
Author(s):  
Xueming Ren ◽  
Ruxin Guo ◽  
Mazarin Akami ◽  
Changying Niu

Nitrogen is usually a restrictive nutrient that affects the growth and development of insects, especially of those living in low nitrogen nutrient niches. In response to the low nitrogen stress, insects have gradually developed symbiont-based stress response strategies—biological nitrogen fixation and nitrogenous waste recycling—to optimize dietary nitrogen intake. Based on the above two patterns, atmospheric nitrogen or nitrogenous waste (e.g., uric acid, urea) is converted into ammonia, which in turn is incorporated into the organism via the glutamine synthetase and glutamate synthase pathways. This review summarized the reaction mechanisms, conventional research methods and the various applications of biological nitrogen fixation and nitrogenous waste recycling strategies. Further, we compared the bio-reaction characteristics and conditions of two strategies, then proposed a model for nitrogen provisioning based on different strategies.


2021 ◽  
Vol 12 ◽  
Author(s):  
Albert Yi-Wey Tan ◽  
Sareena-Hanim Hamzah ◽  
Chih-Yang Huang ◽  
Chia-Hua Kuo

Purpose: This study aimed to assess the requirement of protein in pre-exercise carbohydrate drinks for optimal endurance performance at high intensity and post-exercise fatigue recovery.Methods: Endurance performance at 85% V.⁢O2peak of young men (age 20 ± 0.9 years, V.⁢2peak 49.3 ± 0.3 L/min) was measured for two consecutive days using cycling time to exhaustion and total work exerted 2 h after three isocaloric supplementations: RICE (50 g, protein: 1.8 g), n = 7; SOY + RICE (50 g, protein: 4.8 g), n = 7; and WHEY + RICE (50 g, protein: 9.2 g), n = 7.Results: Endurance performance was similar for the three supplemented conditions. Nevertheless, maximal cycling time and total exerted work from Day 1 to Day 2 were improved in the WHEY + RICE (+21%, p = 0.05) and SOY-RICE (+16%, p = 0.10) supplemented conditions, not the RICE supplemented condition. Increases in plasma interleukin-6 (IL-6) were observed 1 h after exercise regardless of supplemented conditions. Plasma creatine kinase remained unchanged after exercise for all three supplemented conditions. Increases in ferric reducing antioxidant power (FRAP) after exercise were small and similar for the three supplemented conditions.Conclusion: Adding protein into carbohydrate drinks provides no immediate benefit in endurance performance and antioxidant capacity yet enhances fatigue recovery for the next day. Soy-containing carbohydrate drink, despite 50% less protein content, shows similar fatigue recovery efficacy to the whey protein-containing carbohydrate drink. These results suggest the importance of dietary nitrogen sources in fatigue recovery after exercise.


Polymers ◽  
2021 ◽  
Vol 13 (21) ◽  
pp. 3776
Author(s):  
Myrla Melo ◽  
André da Silva ◽  
Edson Silva Filho ◽  
Ronaldo Oliveira ◽  
Jarbas Silva Junior ◽  
...  

In ruminant feeding, mechanisms for controlling the rate of ammonia release in the rumen are important for increasing the efficiency of transforming dietary nitrogen into microbial protein. Three microencapsulated formulations, with increased urea concentrations of 10 (MPec1), 20 (MPec2) and 30% (MPec3) from the w/w, based on the mass of citrus pectin solution, employ the external ionic gelation/extrusion technique. The properties of microencapsulated urea were examined as a completely randomized design with 5 treatments each with 10 replicates for evaluation, and the ratios of dietary to free urea were compared using 5 fistulated male Santa Ines sheep in a Latin 5 × 5 square design. The degradation kinetics showed that the rate of controlled release from the microencapsulated systems was significantly reduced compared with that of free urea (p < 0.05). The population density of ruminal protozoa increased when sheep received the microencapsulated urea (p < 0.05). The disappearance of dry matter and crude protein reached a degradation plateau during the first minutes for the MPec1 and MPec2 systems and was slower for MPec3. The MPec1 and MPec2 systems presented higher (p < 0.05) blood serum concentrations of albumin, urea nitrogen (BUN), creatinine and total cholesterol and did not affect (p > 0.05) the other blood metabolites. The MPec2 systems are recommended because they consist of microspheres with more (p < 0.05) controlled core release, delaying the peak of urea released in the rumen and BUN without affecting (p < 0.05) ruminal pH and temperature. Microencapsulation with calcium pectinate provided better utilization of urea, reducing the risk of ruminant intoxication.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Aaron Mullins ◽  
Thomas Chouvenc ◽  
Nan-Yao Su

AbstractIntrinsic dinitrogen (N2) fixation by diazotrophic bacteria in termite hindguts has been considered an important pathway for nitrogen acquisition in termites. However, studies that supported this claim focused on measuring instant N2 fixation rates and failed to address their relationship with termite colony growth and reproduction over time. We here argue that not all wood-feeding termites rely on symbiotic diazotrophic bacteria for colony growth. The present study looks at dietary nitrogen acquisition in a subterranean termite (Rhinotermitidae, Coptotermes). Young termite colonies reared with wood and nitrogen-rich organic soil developed faster, compared to those reared on wood and inorganic sand. More critically, further colony development was arrested if access to organic soil was removed. In addition, no difference of relative nitrogenase expression rates was found when comparing the hindguts of termites reared between the two conditions. We therefore propose that subterranean termite (Rhinotermitidae) colony growth is no longer restricted to metabolically expensive intrinsic N2 fixation, as the relationship between diazotrophic bacteria and subterranean termites may primarily be trophic rather than symbiotic. Such reliance of Rhinotermitidae on soil microbial decomposition activity for optimal colony growth may also have had a critical mechanistic role in the initial emergence of Termitidae.


2021 ◽  
Vol 8 ◽  
Author(s):  
Hao Li ◽  
Jie Yin ◽  
Xi He ◽  
Zhiqing Li ◽  
Bie Tan ◽  
...  

The study investigated the impact of soybean protein from different processing on the performance, dietary nitrogen digestibility, cecal fermentation characteristics, and bacterial community in newly weaned piglets. The piglets were allocated to two dietary treatment and fed with the extruded full-fat soybean diet (EFS group) and enzyme-treated soybean meal diet (ESBM group), respectively. The piglets in ESBM group showed greater nitrogen digestibility and feed efficiency, and lower diarrhea rate in comparison to piglets in EFS group (P &lt; 0.05). Cecal samples from piglets in ESBM group contained greater concentration of acetate, propionate and total SCFAs (P &lt; 0.05), and lower contents of isobutyrate, isovalerate, total BCFAs, NH3-N and putrescine (P &lt; 0.05) than cecal samples from piglets in the EFS group. The cecal samples from piglets in ESBM group contained greater abundances of g_Blautia, g_Coprococcus_3, g_Fusicatenibacter, and g_Bifidobacterium than the cecal sample from piglets in the EFS group, which could promote to protect intestinal health. In summary, enzyme-treated soybean meal may enhance the growth performance of weaned piglets via increasing the dietary nitrogen digestibility, preventing protein fermentation in the hindgut, which shed light on the mechanism in regulating gut health of dietary protein.


2021 ◽  
Vol 42 (4) ◽  
pp. 2555-2568
Author(s):  
Conrado Trigo Moraes ◽  
◽  
Mirton José Frota Morenz ◽  
Calos Augusto Miranda Gomide ◽  
Domingos Sávio Campos Paciullo ◽  
...  

This study evaluated the effect of energy supplementation with ground corn on the performance of crossbred dairy cows in BRS Kurumi elephant grass pastures managed under rotational stocking during the rainy season. Six Holstein × Gyr cows were used, with average milk production, body weight, body condition score, and days in milk of 18.0±2.89 kg day−1, 560±66 kg, 2.50±0.21, and 99±12, respectively. The experimental design used was the complete reversion (switchback), and the evaluations were conducted over three grazing cycles, with adaptation periods of 14 days and six days of sample collection. The concentrate supplement (ground corn) was supplied twice a day, at a rate of 2 kg cow−1 day−1 in the morning and 1 kg cow−1 day−1 in the afternoon (as-fed basis). A reduction of 23.4% was observed in the pasture dry matter (DM) intake (PDMI) in cows that received energy supplementation, which corresponds to 2.96 kg day-1 less of PDMI when compared to the group without supplementation. This corresponds to a substitution rate of 1.1 kg of pasture per kg of concentrate consumed (DM basis), which reduced the intake (kg cow−1 day−1) of neutral detergent fiber and crude protein by 18.9% and 13.9% in the cows that received ground corn. There were increases of 11.8%, 9.0%, and 10.1%, respectively, in the milk yield, the 3.5% fat-corrected milk yield, and the energy-corrected milk yield of the cows that received ground corn. The response to supplementation in kg of milk per kg of DM of concentrate consumed was 0.57, and the milk contents of fat, protein, and lactose did not differ between treatments. The energy supplementation with 3 kg cow−1 day−1 of ground corn resulted in an increase of 11.8% in the milk yield of Holstein × Gyr dairy cows grazed on BRS Kurumi elephant grass, as well as promoted increases in the daily protein, lactose, and total solids yields. In addition, the provision of 3 kg day−1 of ground corn for lactating cows grazing on BRS Kurumi increased the efficiency in the use of dietary nitrogen.


2021 ◽  
Author(s):  
Matthew D. Regan ◽  
Edna Chiang ◽  
Yunxi Liu ◽  
Marco Tonelli ◽  
Kristen M. Verdoorn ◽  
...  

AbstractHibernation is a mammalian strategy that uses metabolic plasticity to reduce energy demands and enable long-term fasting. Fasting mitigates winter food scarcity but eliminates dietary nitrogen, jeopardizing body protein balance. Here, we reveal gut microbiome-mediated urea nitrogen recycling in hibernating 13-lined ground squirrels (TLGS). Ureolytic gut microbes incorporate urea nitrogen into organic compounds that are absorbed by the host, with the nitrogen reincorporated into the TLGS protein pool. Urea nitrogen recycling is greatest after prolonged fasting in late winter, when urea transporter abundance in gut tissue and urease gene abundance in the microbiome are highest. These results reveal a functional role for the gut microbiome in hibernation and suggest mechanisms by which urea nitrogen recycling contributes to protein balance in other monogastric animals, including humans.One Sentence SummaryGround squirrels and their gut symbionts benefit from urea nitrogen recycling throughout hibernation.


2021 ◽  
Vol 97 (3) ◽  
Author(s):  
Patrick Schimmel ◽  
Lennart Kleinjans ◽  
Roger S Bongers ◽  
Jan Knol ◽  
Clara Belzer

ABSTRACT Human milk stimulates a health-promoting gut microbiome in infants. However, it is unclear how the microbiota salvages and processes its required nitrogen from breast milk. Human milk nitrogen sources such as urea could contribute to the composition of this early life microbiome. Urea is abundant in human milk, representing a large part of the non-protein nitrogen (NPN). We found that B. longum subsp. infantis (ATCC17930) can use urea as a main source of nitrogen for growth in synthetic medium and enzyme activity was induced by the presence of urea in the medium. We furthermore confirmed the expression of both urease protein subunits and accessory proteins of B. longum subsp. infantis through proteomics. To the same end, metagenome data were mined for urease-related genes. It was found that the breastfed infant's microbiome possessed more urease-related genes than formula fed infants (51.4:22.1; 2.3-fold increase). Bifidobacteria provided a total of 106 of urease subunit alpha alignments, found only in breastfed infants. These experiments show how an important gut commensal that colonizes the infant intestine can metabolize urea. The results presented herein further indicate how dietary nitrogen can determine bacterial metabolism in the neonate gut and shape the overall microbiome.


Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 343
Author(s):  
Anna Lavery ◽  
Conrad Ferris

The efficiency with which dairy cows convert dietary nitrogen (N) to milk N is generally low (typically 25%). As a result, much of the N consumed is excreted in manure, from which N can be lost to the environment. Therefore there is increasing pressure to reduce N excretion and improve N use efficiency (NUE) on dairy farms. However, assessing N excretion and NUE on farms is difficult, thus the need to develop proximate measures that can provide accurate estimates of nitrogen utilisation. This review examines a number of these proximate measures. While a strong relationship exists between blood urea N and urinary N excretion, blood sampling is an invasive technique unsuitable for regular herd monitoring. Milk urea N (MUN) can be measured non-invasively, and while strong relationships exist between dietary crude protein and MUN, and MUN and urinary N excretion, the technique has limitations. Direct prediction of NUE using mid-infrared analysis of milk has real potential, while techniques such as near-infrared spectroscopy analysis of faeces and manure have received little attention. Similarly, techniques such as nitrogen isotope analysis, nuclear magnetic resonance spectroscopy of urine, and breath ammonia analysis may all offer potential in the future, but much research is still required.


Sports ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 17
Author(s):  
Ami Mizugaki ◽  
Hiroyuki Kato ◽  
Haruka Suzuki ◽  
Hidefumi Kurihara ◽  
Futoshi Ogita

The protein requirement in athletes increases as a result of exercise-induced changes in protein metabolism. In addition, the frequency, quantity, and quality (i.e., leucine content) of the protein intake modulates the protein metabolism. Thus, this study aimed to investigate whether nutritional practice (particularly, protein and amino acid intake at each eating occasion) meets the protein needs required to achieve zero nitrogen balance in elite swimmers during a training camp. Eight elite swimmers (age 21.9 ± 2.3 years, body weight 64.2 ± 7.1 kg, sex M:2 F:6) participated in a four-day study. The nitrogen balance was calculated from the dietary nitrogen intake and urinary nitrogen excretion. The amino acid intake was divided over six eating occasions. The nitrogen balance was found to be positive (6.7 ± 3.1 g N/day, p < 0.05) with protein intake of 2.96 ± 0.74 g/kg/day. The frequency and quantity of leucine and the protein intake were met within the recommended range established by the International Society of Sports Nutrition. Thus, a protein intake of 2.96 g/kg/day with a well-designated pattern (i.e., frequency throughout the day, as well as quantity and quality) of protein and amino acid intake may satisfy the increased need for protein in an elite swimmer.


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