Ppe.XapF: High Throughput KASP Assays to Identify Fruit Response to Xanthomonas arboricola pv. pruni (Xap) in Peach

Bacterial spot, caused by Xanthomonas arboricola pv. pruni (Xap), is a serious peach disease with symptoms that traverse severe defoliation and black surface pitting, cracking or blemishes on peach fruit with global economic impacts. A management option for control and meeting consumer demand for chemical-free, environmentally friendly fruit production is the development of resistant or tolerant cultivars. We developed simple, accurate, and efficient DNA assays (Ppe.XapF) based on SNP genotyping with KASP technology to quickly test for bacterial spot resistance alleles in peach fruit that allows breeders to cull seedlings at the greenhouse stage. The objective of this research was to validate newly developed DNA tests that target the two major QTLs for fruit resistance in peach with diagnostic utility in predicting fruit response to bacterial spot infection. Our study confirms that only two Ppe.XapF DNA tests, Ppe.XapF1-1 and Ppe.XapF6-2, are needed to distinguish between susceptible and resistant alleles. Use of these efficient and accurate Ppe.XapF KASP tests resulted in 44% reduction in seedling planting rate in the Clemson University peach breeding program.

Evaluation of Chemical Seed Treatment to Reduce Injury Caused by Preemergent Herbicides on Direct-seeded Turnips and Collard Greens

Poor competitive ability and limited herbicide options make weed management of Brassica crops difficult. Growers often adopt the use of transplants, which is less efficient in terms of time, material, and labor when compared with direct seeding, resulting in higher prices per unit. Seed treatment with protective compounds could decrease crop injury from preemergent (PRE) herbicides making it profitable to direct-seed Brassica plants for production. Research was conducted to evaluate the ability of three candidate safeners [24-epibrassinolide, melatonin, and ascorbic acid (AsA)] to reduce injury caused by four herbicides (S-metolachlor, pyroxasulfone, halosulfuron, and mesotrione) applied PRE on the collard green cultivar Top Bunch and turnip cultivar Purple Top White Globe. Two independent greenhouse trials were conducted at the Clemson University Coastal Research and Education Center in Charleston, SC. Visual injury of the treated plants was evaluated weekly and dry mass was collected 21 days after treatment. Seed treatment did not reduce injury efficiently caused by pyroxasulfone, halosulfuron, and mesotrione; all doses were lethal for both crops. However, collard seeds treated using melatonin and AsA had 66% and 54% less injury caused by S-metolachlor at 514 g⋅ha–1 a.i., respectively. On turnips, melatonin was the only treatment that reduced the S-metolachlor damage on seedlings, with 43% less injury than untreated seedlings. Plant injury and plant weight correlated significantly for both Brassica crops. The reduction in injury caused by S-metolachlor when seeds were treated with melatonin and AsA validated those compounds’ protective ability. Seed treatment with melatonin could be combined with PRE applications of S-metolachlor to overcome the low weed competitive ability of these species early in the season.

Cancer mapping pilot study of cancer association patients in Anderson County, SC

This study represents a collaboration between the Cancer Association of Anderson (CAA) and the Anderson University Center for Cancer Research (AU CCR), both located in Anderson, SC, with the assistance and support of Anderson University Professional and Lifelong Learning (AU PLL). The CAA began importing patient information in 2016 into a Microsoft Excel workbook to create a digital record that could store and provide more convenient searching and the production of reports. In a robust collaborative effort between CAA, the AU CCR, and Anderson University students, additional pertinent information from the previous paper files was gathered by contacting patients. The data was imported from the Excel system and put into a reporting system that allowed CAA staff and AU CCR students to refine the information needed to obtain an overview of cancer across Anderson County as a snapshot of those assisted by CAA. The Clemson University GIS data system and the database reports were used to plot the location of cancer incidents (the individual patient's locations) and to produce maps that aided the students in drawing initial conclusions. By working collaboratively from 2019 through 2020, the CAA and AU students have gathered local Anderson County cancer patient information and created maps to pinpoint different cancers by location. These maps indicate geographic trends of various cancers and support further investigation into the cause of those cancers. They also indicate increased or decreased rates of cancer over time.

Reviewer Acknowledgements

Journal of Agricultural Studies (JAS) would like to acknowledge the following reviewers for their assistance with peer review of manuscripts for this issue. Many authors, regardless of whether JAS publishes their work, appreciate the helpful feedback provided by the reviewers. Their comments and suggestions were of great help to the authors in improving the quality of their papers. Each of the reviewers listed below returned at least one review for this issue.Reviewers for Volume 9, Number 3Ahmad Reza Pirali Zefrehei, Gorgan University, IranAlessandra M. Lima Naoe, Federal University of Tocantins, BrazilAlexandru Ioan Apahidean, Univ. of Agricultural Sciences and Veterinary Medicine, RomaniaAna Daniela Lopes, Universidade Paranaense, BrazilAnca-Luiza Stanila, ICPA, RomaniaAndré Luiz Rodrigues Magalhães, UFAPE, BrazilAngel Ramon Sanchez Delgado, Universidade federal Rural do Rio de Janeiro, BrazilArnaud Z. Dragicevic, IRSTEA, FranceBenard Kiplangat Rop, University of Nairobi, KenyaCamilla H. M. Camargos, University of Campinas, BrazilCleber Duarte, University of Guararapes, BrazilDarwin Pangaribuan, University of Lampung, IndonesiaEben von Well, Agricultural Research Council, South AfricaEliana Mariela Werbin, National University of Cordoba, ArgentinianEmmanuel E. Omeje, University of Nigeria, NigeriaEric Krawczyk, University of Michigan, USAEric Owusu Danquah, CSIR-Crops Research Institute, GhanaFernando Rodrigues de Amorim, State University of Paulista (UNESP), BrazilGuitong Li, China Agricultural University, ChinaHabu Saleh Hamisu, National Horticultural Research Institute, NigeriaHedayatollah K. Soureshjani, Ferdowsi University of Mashhad, IranJacquelin Teresa Camperos Reyes, São Paulo State University (UNESP), BrazilJorge A. López, University Tiradentes, BrazilJuliana Nneka Ikpe, Akanu Ibiam Federal Polytechnic, NigeriaLuh Suriati, Warmadewa University, IndonesiaMahyar Gerami, Sana Institute of Higher Education, IranMaría Francisca Perera, ITANOA, EEAOC-CONICET, ArgentinaMariana Esteves, Luiz de Queiroz College of Agriculture University of Sao Paulo, BrazilMohamed Mattar, King Saud University, Saudi ArabiaMohammed El Basuini, Kagoshima University, JapanMohammed Jamal Uddin, Bangladesh Agricultural Research Institute (BARI), BangladeshNkemkanma Vivian Agi, Rivers State University Port Harcourt, NigeriaOlga Mykhailenko, National University of Pharmacy, UkraineRadu Liviu Sumalan, University of Agricultural Sciences and Veterinary, RomaniaRaul Pașcalău, Banat's University, RomaniaSaiful Irwan Zubairi, Universiti Kebangsaan Malaysia (UKM), MalaysiaSarir Ahmad, Abdul Wali Khan University, PakistanShubha Kumari, ICAR-RCER, IndiaSina Nabaei, Azad University, IranSudu Hakuruge Pushpa Malkanthi, Sabaragamuwa University of Sri Lanka, Sri LankaSybelle Mesquita Silva, Universidade Federal de Alagoas, BrazilToncho Gospodinov Penev, Trakia University, BulgariaTugay Ayasan, Osmaniye Korkut Ata University, TurkeyUtkarsh R. Moon, Mahatma Gandhi College of Science, IndiaZakaria Fouad Abdallah, National Research Centre, EgyptZeinab Mohammadi Shad, Iowa State University, USAZhao Chen, Clemson University, USA Richard WilliamsEditorial AssistantJournal of Agricultural Studies--------------------------------------Macrothink Institute5348 Vegas Dr.#825Las Vegas, Nevada 89108United StatesPhone: 1-702-953-1852 ext.521Fax: 1-702-420-2900Email 1: [email protected] 2: [email protected]: http://jas.macrothink.org

Peer commentary of the paper "Does Proximity to a Golf Course Matter?" by Owusu-Eudsei, K. & Espey, M. (2003). Working Papers of the Agricultural and Applied Economics Department at Clemson University (WP 012203)

Peer commentary on the paper "Does Proximity to a Golf Course Matter?".