A Novel Approach to Develop Lager Yeast with Higher NADH Availability to Improve the Flavor Stability of Industrial Beer

Flavor stability is important for beer quality and extensive efforts have been undertaken to improve this. In our previous work, we proved a concept whereby metabolic engineering lager yeast with increased cellular nicotinamide adenine dinucleotide hydride (NADH) availability could enhance the flavor stability of beer. However, the method for breeding non-genetically modified strains with higher NADH levels remains unsolved. In the current study, we reported a novel approach to develop such strains based on atmospheric and room temperature plasma (ARTP) mutagenesis coupled with 2,4-dinitrophenol (DNP) selection. As a result, we obtained a serial of strains with higher NADH levels as well as improved flavor stability. For screening an optimal strain with industrial application potential, we examined the other fermentation characteristics of the mutants and ultimately obtained the optimal strain, YDR-63. The overall fermentation performance of the strain YDR-63 in pilot-scale fermentation was similar to that of the parental strain YJ-002, but the acetaldehyde production was decreased by 53.7% and the resistance staling value of beer was improved by 99.8%. The forced beer aging assay further demonstrated that the favor stability was indeed improved as the contents of 5-hydroxymethylfurfural in YDR-63 was less than that in YJ-002 and the sensory notes of staling was weaker in YDR-63. We also employed this novel approach to another industrial strain, M14, and succeeded in improving its flavor stability. All the findings demonstrated the efficiency and versatility of this new approach in developing strains with improved flavor stability for the beer industry.

Development of craft Beer with pecan shell aqueous extract [(Carya illinoensis) (Wangenh) C. Koch]

Different ingredients and adjuncts have been used in the production of craft beer aiming to add new characteristics to the final product. In turn, the pecan nut has recognized antioxidant properties and, therefore, the extract of its shell was added in the beer production process with the main objective of increasing the beer antioxidant capacity. The all-grain method was used to produce the beverage and, on the packaging step, pecan nutshell extract was added at three different concentrations. The samples underwent antioxidant, physicochemical, and sensorial analysis. Data were submitted to analysis of variance (ANOVA). The use of pecan nutshell extract as an ingredient in beer processing has proved to be a viable alternative due to satisfactory results in sensory evaluation, with good acceptability of the consumers, maintaining physicochemical characteristics similar to the standard beer produced without the extract. However, the addition of the extract did not increase the antioxidant capacity in craft beer. Even though the antioxidant power of produced beer wasn´t increased in this experiment, still pecan nutshell could be a good resource of antioxidants to whom drink beer, and your low price in the market make it viable to beer industry.

The effect of competition on product removal

The relationship between innovation and competition has been vastly studied over the past fifty years. However, one piece of the puzzle that has not been studied in detail is how in certain industries competition has an effect in the number of products that are removed from the market. That is why in this paper, I use scanner data to analyze the effect of competition on product removal. In particular, I track sales in the beer industry across a set of 1107 over a period of four years. Following previous studies, I use the merger between SABMiller and Molson Coors as an unexpected change in the industry to estimate future market concentration. I find that there is a negative relationship between removal and concentration. Therefore, retailers decide to remove products from their shelves in a faster way when the market is more competitive to open the gates for a more dynamic assortment.

Oligopolistic Price Leadership and Mergers: The United States Beer Industry

We study a repeated game of price leadership in which a firm proposes supermarkups over Bertrand prices to a coalition of rivals. Supermarkups and marginal costs are recoverable from data on prices and quantities using the model’s structure. In an application to the beer industry, we find that price leadership increases profit relative to Bertrand competition by 17 percent in fiscal years 2006 and 2007, and by 22 percent in 2010 and 2011, with the change mostly due to consolidation. We simulate two mergers, which relax binding incentive compatibility constraints and increase supermarkups. These coordinated effects arise even with efficiencies that offset price increases under Bertrand competition. (JEL G34, K21, L13, L14, L41, L66)

Fungal Biovalorization of a Brewing Industry Byproduct, Brewer’s Spent Grain: A Review

The beer industry is a major producer of solid waste globally, primarily in the form of brewer’s spent grain (BSG), which due to its low value has historically been diverted to livestock as feed or to landfills. However, its high moisture content and chemical composition positions BSG as an ideal candidate for further processing with microbial fermentation. Recent research has focused on filamentous fungi and the ability of some species therein to degrade the predominant recalcitrant cellulolignin components of BSG to produce valuable compounds. Many species have been investigated to biovalorize this waste stream, including those in the genuses Aspergillus, Penicillium, Rhyzopus, and Trichoderma, which have been used to produce a wide array of highly valuable enzymes and other functional compounds, and to increase the nutritional value of BSG as an animal feed. This review of recent developments in the application of filamentous fungi for the valorization of BSG discusses the biochemical makeup of BSG, the biological mechanisms underlying fungi’s primacy to this application, and the current applications of fungi in this realm.

Beer Industry in the Czech Republic: Reasons for Founding a Craft Brewery

The goal of this article is to evaluate the evolution of the brewing industry in the Czech Republic with an emphasis on the phenomenon of craft-brewery development. It deals with the influence of globalization on the structure of the Czech beer market and the rise of craft-breweries between 2000 and 2019. The main outputs come from research where a representative sample of 48 craft breweries was questioned from the Czech Republic. The result is the identification of the main factors influencing the increase of craft-breweries (legislation changes enabling entrepreneurship, increase of purchasing power of consumers, increase in demand for different beer styles, craft beers and specials, change of consumer behavior) but also the challenges that prevent their further expansion (lack of qualified brewers, complicated administration). The main motive for founding a craft brewery is an effort to improve beer culture in the Czech Republic and the ever-increasing demand for diversified beer (as opposed to the demand for the so-called euro-beers) and a good business opportunity stemming from this, which has been attracting more and more investors into this field.

How to Install Twines in a Hopyard

Because of the rapidly growing craft beer industry and rising hop prices, the potential of hops as an alternative crop is currently being investigated at the UF/IFAS GCREC. Proper installation of twines is critical to facilitate easier crop management and harvesting. This new article by explains how to install twines on a 18-ft hop trellis. It is part of a series that reviews the challenges of hop production in Florida. Written by Shinsuke Agehara and Christopher DelCastillo; 3 pp.https://edis.ifas.ufl.edu/hs1418