Dental Pulp Stem Cell Polarization: Effects of Biophysical Factors

Dental pulp stem cells (DPSCs) have the potential to polarize, differentiate, and form tubular dentin under certain conditions. However, the factors that initiate and regulate DPSC polarization and its underlying mechanism remain unclear. Identification of the factors that control DPSC polarization is a prerequisite for tubular dentin regeneration. We recently developed a unique bioinspired 3-dimensional platform that is capable of deciphering the factors that initiate and modulate cell polarization. The bioinspired platform has a simple background and confines a single cell on each microisland of the platform; therefore, it is an effective tool to study DPSC polarization at the single-cell level. In this work, we explored the effects of biophysical factors (surface topography, microisland area, geometry, tubular size, and gravity) on single DPSC polarization. Our results demonstrated that nanofibrous architecture, microisland area, tubular size, and gravity participated in regulating DPSC polarization by influencing the formation of the DPSC process and relocation of the Golgi apparatus. Among these factors, nanofibrous architecture, tubular size, and appropriate microisland area were indispensable for initiating DPSC polarization, whereas gravity served as an auxiliary factor to the process of DPSC polarization. Meanwhile, microisland geometry had a limited effect on DPSC polarization. Collectively, this work provides information on DPSC polarization and paves the way for the development of new biomaterials for tubular dentin regeneration.

Flooding Hazard and Vulnerability. An Interdisciplinary Experimental Approach for the Study of the 2016 West Virginia Floods

The hydrosocial (HS) and social-hydro (SH) frameworks each attempt to understand the complexity of water and society, but they have emerged from historically disparate fields with distinctly different goals as well as methodological and epistemological standpoints. This paper encapsulates the shared experiences of two human geographers and two hydrologists studying hazard and vulnerability in two communities impacted by extreme flooding in West Virginia in 2016. We add to the limited examples of scientists working across epistemologies to improve the understanding of water-societal relations. In so doing, we also contribute to broader discussions of water justice. We outline an experimental approach connecting hydrosocial and social-hydro frameworks to study flood hazard and vulnerability. Within our conceptualization, we set forth that while social and hydrological factors can be presented as purely anthropogenic or geophysical, respectively, their intersection is the crux to investigate. The relationships between variables of both major categories can help us understand how the social and biophysical systems are interrelated. We depart from 21 semi structured interviews and a secondary analysis of local biophysical factors to develop a model that could show the relations between social and biophysical factors. Linking these factors is crucial step toward integration of SH and HS approaches to create a more comprehensive understanding of water-human relations. These studies can inform policymakers by highlighting where negative connections can be remedied and positive connections can be fostered to emphasize water justice.

Kirlian Experimental Analysis and IoT

The energy measurement of different organs of the human body is essential and can be used as a warning indication of existing and future possible health issues. The pranic energy or aura of human body does not merely affect individual to himself or herself, but also it has drastic impressions on others too in a certain extent. Few studies have been done towards this direction. The article is the second part of the total content. It is an effort by the research team to logically discuss the human bioelectricity and measure the different biophysical factors and reasons for various ill symptoms. The authors have applied health sensors to capture the massive data and process it through IoT applications and concepts and subsequently compared for two subjects.

Uncovering the Role of Biophysical Factors and Socioeconomic Forces Shaping Soil Sensitivity to Degradation: Insights from Italy

Following an operational framework derived from earlier research, our study research estimates the specific contribution of biophysical and socioeconomic factors to soil sensitivity to degradation at two-time points (Early-1990s and Early-2010s) in Italy, a Mediterranean hotspot for desertification risk. A total of 34 variables associated (directly or, at least, indirectly) with different processes of soil degradation (erosion, salinization, sealing, contamination, and compaction) and climate change were considered here, delineating the predominant (underlying) cause (i.e., biophysical or socioeconomic). This set of variables represented the largest (quantitative) information available from national and international data sources including official statistics at both national and European scale. Contribution of biophysical and socioeconomic dimensions to soil sensitivity to degradation was heterogeneous in Italy, with the level of soil sensitivity to biophysical factors being the highest in less accessible, natural areas mostly located in hilly and mountainous districts. The highest level of soil sensitivity to socioeconomic drivers was instead observed in more accessible locations around large cities and flat rural districts with crop intensification and low (but increasing) population density. All these factors delineated an enlarged divide in environmental quality between (i) flat and upland districts, and between (ii) Northern and Southern Italian regions. These findings suggest the appropriateness of policy strategies protecting soils with a strong place-specific knowledge, i.e., based on permanent monitoring of local (biophysical and socioeconomic) conditions.