The Species Composition of Microbiota and Biological Properties of Dominant Bacterial Species in the Lacrimal Fluid in Patients with Endogenous Uveites

The purpose: to study the species composition of the lacrimal fluid microbiota in patients with endogenous uveitis and the biological properties of dominant species of bacteria.Patients and Methods. A bacteriological study of the lacrimal fluid of 107 patients (114 eyes) with endogenous uveitis and 28 practically healthy persons (control) was carried out. The research was performed using the conventional methods. The isolated microorganisms were identified up to the species. We studied the main biological properties of bacteria — the presence of persistence and virulence factors of isolated dominant species.The results. 43 strains of various types of bacteria were isolated from the lacrimal fluid of patients with uveitis, bacteria of the genus Staphylococcus prevailed, S. aureus was isolated in 23.3 % of cases, and the remaining strains were representatives of coagulase-negative staphylococci. The species composition of the bacteria isolated from practically healthy individuals did not differ in essence. Isolated strains of S. aureus had a significant set of virulence factors. All isolates lysed sheep red blood cells, 80 % of the cultures had lecithovitellase and 60 % — DNA-se activity. Representatives of coagulase-negative staphylococci were characterized only by hemolytic activity. In the study of persistence factors, it was found that all the studied strains of S. aureus and the vast majority of representatives of coagulase-negative staphylococci isolated from tears were resistant to lysozyme. Biofilm formation was more typical for S. aureus strains 90 % versus 34.4 % for representatives of coagulase-negative staphylococci (t = 2.194, p = 0.034). Similar patterns were found in relation to complement resistance, 90 % of S. aureus cultures and 37.5 % of coagulase-negative staphylococci (t = 2.055; p = 0.046).Conclusion. Among the microorganisms isolated from the lacrimal fluid, Staphylococcus bacteria prevailed, from which S. cohnii was most often isolated. S. aureus to a greater extent than coagulase-negative staphylococci had a certain set of virulence and persistence factors. The microbiocenosis of the lacrimal fluid of patients with endogenous uveitis did not differ from practically healthy individuals. The presence of S. aureus in lacrimal fluid is a risk factor for the development of endogenous uveitis in cases of chronic oral and ENT organ diseases.

Pengaruh School Climate dan Persistence terhadap Counterproductive Behavior (Perilaku Menyimpang terhadap Pelestarian Lingkungan) Siswa SMA

Environmental problems cause threats to environmental sustainability. This problem can be caused by deviant behavior towards environmental preservation or counterproductive behavior. This study aims to determine the influence of school climate and persistence on counterproductive behavior student related to environmental sustainability. The method used is a survey method through causal studies and analyzed by path analysis. The survey was conducted on 70 students at SMAN 48 Jakarta in the odd semester of the 2020/2021 school year. The calculation result of the instrument reliability coefficient is counterproductive behavior 0.782, the school climate is 0.850, and the persistence is 0.917 which indicates that the three instruments can be trusted. The results showed that school climate directly affected persistence significantly, persistence directly affected counterproductive behavior significantly, school climate directly affected counterproductive behavior significantly, and obtained good persistence results as a mediator variable between school climate and counterproductive behavior in students. Therefore, in an effort to reduce counterproductive behavior student, school climate and student persistence factors also need to be considered.

Etiopathogenetic specificity and prospects of phage therapy in infectious keratoconjunctivitis in cattle

Infectious bovine keratoconjunctivitis (IBK) is a bacterial eye disease affecting cattle worldwide. The main etiological role in IBK belongs to Moraxella spp. (including in associations with Staphylococcus spp., Streptococcus spp., Pseudomonas aeruginosa, E. coli, Proteus). Persistence factors and antimicrobial resistance of these bacteria, reduce the effectiveness of traditional treatment measures for IBK. New strategies for combating bacterial infections set up prospects for the use of bacteriophages. The paper presents data substantiating the relevance of the development of a therapeutic and prophylactic method of phage processing and an agent based on lytically properties of bacteriophages to combat infectious keratoconjunctivitis in cattle.

КУРМАНЖАН ДАТКА ЖАНА СТУДЕНТ КЫЗДАРДЫН ЛИДЕРДИК ӨЗГӨЧӨЛҮКТӨРҮ

Аннотация: Бул илимий изилдөө ишинин максаты – Алай ханышасы Курманжан даткадагы лидердик өзгөчөлүктөрү жана кыргыз кыздарындагы лидер аялдардын сапаттарынын изилденүүсү максатталды. Максатка жетүү үчүн “Курманжан датка” фильмине мазмундук талдоо жана 2017-жылы декабрь айында Бишкек шаарындагы Кыргыз-Түрк «Манас», И.Арабаев жана К.Карасаев атындагы Бишкек мамлекеттик университетинде билим алган 186 студент кыздарга сурамжылоо жүргүзүлгөн. Изилдөөнүн жыйынтыгында студент кыздардан өзүнө ишенимдүүлүк, коммуникация жана өжөрлүк факторлору аныкталды жана КТМУнун студент кыздарынын башка жогорку окуу жайларынын студент кыздарына караганда өзүнө ишенимдүүлүктөрү төмөн болгондугу аныкталды. Аннотации: Целью данного исследования было изучение лидерских качеств Алайской царицы Курманжан Датка и качеств женщин-лидеров среди кыргызских девушек. Для достижения этой цели проведен контент-анализ к фильму «Курманжан датка» и был проведен опрос среди 186 студенток Кыргызско-Турецкого Университета «Манас, КГУ им. И. Арабаева и БГУ им. К. Карасаева в декабре 2017 года. Исследование выявило среди студентов такие факторы как уверенность в себе, коммуникабельность и настойчивость и обнаружило, что студентки КТМУ имеют относительно низкую уверенность в себе, чем студентки других университетов. Abstract: The purpose of this paper is to research leadership features of the Queen of Alay Kurmanjan Datka and Kyrgyz young leadership skills. The research focuses of a film on “Kurmanjan Datka” and at the end of 2017 in the city of Bishkek, Kyrgyz-Turkish University of “Manas”, I. Arabayev and Humanitar University of K. Karasayev 186 female students were surveyed. The result of interiew’s reported the female students hadself-confidence, communication and persistence factors. The results showed KTMU’s female students had relatively low self-confidence compared to other universities female students.

Eveningness Diurnal Preference: Putting the “Sluggish” in Sluggish Cognitive Tempo

Objective: Eveningness diurnal preference is common in psychiatric conditions, including attention-deficit/hyperactivity disorder (ADHD) and internalizing disorders. Little is known about how diurnal preference relates to sluggish cognitive tempo (SCT)—a distinct clinical construct associated with functional impairment—in clinical samples. Method: Adult outpatients ( n = 65; 43 with ADHD, 22 with internalizing/adjustment disorders) self-reported on SCT symptoms (total symptoms; slow/daydreamy, sleepy/sluggish, and low initiation/persistence factors) and diurnal preference. Results: Greater eveningness was associated with overall SCT severity and sleepy/sluggish symptoms in the full sample. Relationships between eveningness and overall SCT severity and slow/daydreamy symptoms were stronger for those with internalizing/adjustment disorders compared to ADHD. The relationship between eveningness and sleepy/sluggish symptoms was uniform across groups. Conclusion: Findings suggest a potential role of eveningness preference in adult SCT presentation. Future studies should investigate underlying mechanisms linking these two constructs and the efficacy of circadian interventions in the treatment of SCT among adult outpatients.

Issues beyond resistance: inadequate antibiotic therapy and bacterial hypervirulence

ABSTRACT The administration of antibiotics while critical for treatment, can be accompanied by potentially severe complications. These include toxicities associated with the drugs themselves, the selection of resistant organisms and depletion of endogenous host microbiota. In addition, antibiotics may be associated with less well-recognized complications arising through changes in the pathogens themselves. Growing evidence suggests that organisms exposed to antibiotics can respond by altering the expression of toxins, invasins and adhesins, as well as biofilm, resistance and persistence factors. The clinical significance of these changes continues to be explored; however, it is possible that treatment with antibiotics may inadvertently precipitate a worsening of the clinical course of disease. Efforts are needed to adjust or augment antibiotic therapy to prevent the transition of pathogens to hypervirulent states. Better understanding the role of antibiotic-microbe interactions and how these can influence disease course is critical given the implications on prescription guidelines and antimicrobial stewardship policies.

PERSISTENT POTENTIAL OF PROTOZOA BLASTOCYSTIS SPP.

In recent years, the parasitic protozoan Blastocystis spp., inhabiting in the human gastrointestinal tract, has begun to attract more and more attention on the site of parasitologists. A high incidence of blastocyst contamination was revealed in individuals of various population groups. In this paper, the results of the study showing high rates of invasiveness of foundry workers by the above-mentioned protozoan, characterized by hazardous working conditions of both physical and chemical nature. The ability of protozoa to inhabit a particular ecological niche depends on their biological properties; thus, persistence factors are of particular interest. The article describes the persistent potential of Blastocystis spp., inhabiting the human intestines. Persistent properties in prokaryotes is a significant physiological factor for their existence and determine the mechanism of interaction of microorganisms that form the intestinal microflora. The complex of persistence factors (ALA, ALFA, and AHA) of Blastocystis spp. was studied. A direct correlation between the persistent activity of the blastocysts and the severity of intestinal microbiocenosis disorders was found. The effect of destabilizing production factors leading to the restructuring of the intestinal biome has been established, contributing to a decrease in the indigenous group of microorganisms and an increase of opportunistic flora. The received data allow using the most important biological properties of the survival of

Exploring Persistence Factors Of Students in Agricultural Science

The authors employed a qualitative case study design to explore factors of persistence in agricultural science program. Specifically focused on factors that influence participants continue in enroll the agricultural program in tertiary education since previously studied a subject or several subjects related to agriculture in secondary school. All participants had taken agricultural programs in tertiary education and previously had studied subject that related to agriculture during secondary school. Findings show that support from close individual, school environment, exposure to agriculture and personal factor were factors influencing the persistence of students in agriculture. The authors address implications for increasing student’s persistence in agricultural science program.

Interpreting Rural Students’ Stories of Access to a Flagship University

Access has been an ongoing issue for rural students. In this study, I examined factors that have been proven barriers and supports for rural students. Rural college students who were part of the TRiO program at a flagship university shared barriers and supports to access higher education. Students talked about their college journeys by telling digital stories, using computer-based tools to create narratives. On-campus resources, family support, finding a place to belong in college, and self-efficacy proved to be important access and persistence factors for students.