INVESTIGATION OF LIGHT INTENSITY OF LIGHT CURING UNITS AFTER DIFFERENT PERIODS OF USE

Quartz-tungsten halogen light curing units (LCUs) have been the main source of light for the polymerization of resin based composites (RBCs) for several decades. Since the beginning of the 20th century, however, their use has been reduced due to the invention and improvement of LED LCUs. Various factors can cause a decrease in the light intensity of LED LCUs, one of which is diode aging. The aim of the present paper is to study the change in light intensity of LCUs after different periods of intensive use. For this purpose, the light intensity of 94 regularly used LED LCUs aged between 1 and 10 years was measured with a digital radiometer. The devices were used in conventional mode with maximum light intensity. It was found that regardless of the type and model of LCU, there is a direct relationship between the time of use and light intensity - the longer the operation period of a device is and the more used it is, the lower its intensity is. The decrease in light intensity as devices age is different for different models, as well as for different devices of the same model. In the studied LCUs with a 10-year period of use, 77.5% have light intensity lower than the required minimum of 400 mW/cm2, which makes them unusable. It can be concluded that dentists should regularly monitor and measure the light intensity of their LCUs, especially as they age, to ensure the longevity of their restorative procedures.

Photocataytic Degradation of Phenol Using TiO2-Fe Under H2O2 Presence by Visible and Sunlight Irradiation

Phenol is one of the essential organic pollutants released into the environment because of its high stability and toxicity. It is harmful to organisms, environment, and posing a serious threat to human health at low concentration. This research investigated the photocatalytic degradation process of phenol using a TiO2-Fe catalyst under visible light irradiation and additional H2O2. The effect of various conditions process was applied, including different catalyst doses (0.2, 0.4, 0.6, and 0.8 g/L), pH (3, 6, 8, and 11), irradiation times (60, 90, 120, 150, and 210 minutes) and the presence of H2O2. The degradation process was studied at an initial concentration of phenol 5 mg/L. This study has been decreasing phenol content (90.51%) with catalyst doses 0.6 g/ L sample solution, pH solution 11, reaction time 210 minutes and H2O2 concentration 30%. This final phenol concentration after photodegradation under halogen light was 0.18 mg/L, while sunlight irradiation was 0.11 mg/L. This result is below government regulation as per Permen LH RI No. 5/2014 i.e. 0.5 mg/L. Therefore, this process possible to remove phenol in aqueous such as industrial wastewater or other resources.

Effect of Light-Sources and Thicknesses of Composite Onlays on Micro-Hardness of Luting Composites

The aim of this study was to compare three different light-curing-units (LCUs) and determine their effectiveness in the adhesive cementation of indirect composite restorations when a light-curing resin cement is used. Two resin composites were selected: Enamel Plus HRI (Micerium) and AURA (SDI). Three thicknesses (3 mm, 4 mm and 5 mm) were produced and applied as overlays and underlays for each resin composite. A standardized composite layer was placed between underlay and overlay surfaces. Light curing of the resin-based luting composites was attained through the overlay filters using LCUs for different exposure times. All specimens were allocated to experimental groups according to the overlay thickness, curing unit and curing time. Vickers Hardness (VH) notches were carried out on each specimen. Data were statistically evaluated. The curing unit, curing time and overlay thickness were significant factors capable of influencing VH values. The results showed significantly decreased VH values with increasing specimen thickness (p < 0.05). Significant differences in VH values were found amongst the LCUs for the various exposure times (p < 0.05). According to the results, a time of cure shorter than 80 s (with a conventional quartz–tungsten–halogen LCU) or shorter than 40 s (with a high-power light-emitting diode (LED) LCU) is not recommended. The only subgroup achieving clinically acceptable VH values after a short 20 s curing time included the 3 mm-thick overlays made out of the AURA composite, when the high-power LED LCU unit was used (VH 51.0). Composite thickness has an intense effect on polymerization. In clinical practice, light-cured resin cements may result in insufficient polymerization for high thickness and inadequate times. High-intensity curing lights can attain the sufficient polymerization of resin cements through overlays in a significantly shorter time than conventional halogen light.

Silicon Particles/Black Paint Coating for Performance Enhancement of Solar Absorbers

The availability of fresh drinkable water and water security is becoming a global challenge for sustainable development. In this regard, solar stills, due to their ease in operation, installation, and utilization of direct sunlight (as thermal energy), promise a better and sustainable future technology for water security in urban and remote areas. The major issue is its low distillate productivity, which limits its widespread commercialization. In this study, the effect of silicon (Si) particles is examined to improve the absorber surface temperature of the solar still absorber plate, which is the major component for increased distillate yield. Various weight percentages of Si particles were introduced in paint and coated on the aluminum absorber surface. Extensive indoor (using a self-made halogen light-based solar simulator) and outdoor testing were conducted to optimize the concentration. The coatings with 15 wt % Si in the paint exhibited the highest increase in temperature, namely, 98.5 °C under indoor controlled conditions at 1000 W/m2 irradiation, which is 65.81% higher than a bare aluminum plate and 37.09% higher compared to a black paint-coated aluminum plate. On the other hand, coatings with 10 wt % Si reached up to 73.2 °C under uncontrolled outdoor conditions compared to 68.8 °C for the black paint-coated aluminum plate. A further increase in concentration did not improve the surface temperature, which was due to an excessive increase in thermal conductivity and high convective heat losses.

Measuring aggregate electricity savings from the diffusion of more efficient lighting technologies

Increasing concerns about sustainability and energy conservation, coupled with the proliferation of incentives in the EU to achieve energy savings, suggest that significant improvements in energy efficiency should be realized. A policy measure that should have a direct impact on energy savings is the replacement of incandescent and halogen light bulbs by more efficient lighting technologies, which was implemented in 2009. Due to the lack of detailed data, it is not feasible to measure the effect of energy-efficient improvements on electricity consumption at the aggregate level using a bottom-up approach. To overcome this limitation, this paper analyzes hourly electricity demand in a very specific period of the day: the transition from day to night. In this short period, it is plausible that lighting is the main driver of changes in electricity demand, thus making it possible to estimate the increase in electricity consumption when lights are switched on and to analyze the effects of higher energy efficiency in lighting, if any. The results of the analysis for Spain show that during the periods 2009–2011 and 2015–2016, an estimated energy savings of 251 GWh can be attributed to a reduction in the magnitude of the lighting effect, which accounts for 20.3% of the observed decrease in electricity consumption during these two periods.

Molecular Assay Development to Monitor the Kinetics of Viable Populations of Two Biocontrol Agents, Bacillus subtilis QST 713 and Gliocladium catenulatum J1446, in the Phyllosphere of Lettuce Leaves

Optimising the use of biocontrol agents (BCAs) requires the temporal tracking of viable populations in the crop phyllosphere to ensure that effective control can be achieved. No sensitive systems for quantifying viable populations of commercially available BCAs, such as Bacillus subtilis and Gliocladium catenulatum, in the phyllosphere of crop plants are available. The objective of this study was to develop a method to quantify viable populations of these two BCAs in the crop phyllosphere. A molecular tool based on propidium monoazide (PMA) (PMAxx™-qPCR) capable of quantifying viable populations of these two BCAs was developed. Samples were treated with PMAxx™ (12.5–100 μM), followed by 15 min incubation, exposure to a 800 W halogen light for 30 min, DNA extraction, and quantification using qPCR. This provided a platform for using the PMAxx™-qPCR technique for both BCAs to differentiate viable from dead cells. The maximum number of dead cells blocked, based on the DNA, was 3.44 log10 for B. subtilis and 5.75 log10 for G. catenulatum. Validation studies showed that this allowed accurate quantification of viable cells. This method provided effective quantification of the temporal changes in viable populations of the BCAs in commercial formulations on lettuce leaves in polytunnel and glasshouse production systems.

Investigation of microleakage of polymerized with LED and halogen light devices four different restorative materials

Aim: This study aimed to evaluate the in vitro effects on microleakage of LED and halogen light devices used in the polymerization of monomer structure composite resins of different viscosities and inorganic filling particle size applied to standard class V cavities in primary teeth. Methodology: 80 non caries primary molar teeth with standard class v cavity on the buccal surfaces were used. The teeth were randomly divided into 4 main groups and restored with composite resins (Herculite® XRV, Ultra™, Filtek™ Silorane, Vertise™ Flow, Æliteflo™). Each group was divided into 2 sub-groups for polymerization with LED or halogen light devices. Following the thermal cycle and subsequent procedures, the dye penetration method was used to evaluate microleakage. The microleakage scores were evaluated using the Kruskal-Wallis and Mann-Whitney U-tests. Results: According to the results of the statistical analysis, in polymerization made with halogen and LED light devices at the occlusal edge, the microleakage scores from lowest to highest were as follows: Filtek™ Silorane < Herculite® XRV Ultra™ < Æliteflo™ < Vertise™ Flow. In polymerization made with halogen and LED light devices at the gingival edge, the microleakage scores from lowest to highest were as follows: Filtek™ Silorane < Herculite® XRV Ultr™ < Vertise™ Flow < Æliteflo™. Conclusion: In the polymerizations made by using LED and halogen light devices, Herculite® XRV Ultra™, was found to be successful as it showed similar values to Filtek™ Silorane, which gave the best results in terms of microleakage. Moreover, as we have reached similar findings in our thesis study in respect of microleakage, in cases indicating the use of flow composite resin materials such as Æliteflo™, Vertise™ Flow can be used as it has the advantage of ease application and thus provide an ideal alternative in pediatric dentistry. How to cite this article: Günay A, Tümen EC. Investigation of microleakage of polymerized with LED and halogen light devices four different restorative materials. Int Dent Res 2020;10(3):73-9. https://doi.org/10.5577/intdentres.2020.vol10.no3.2 Linguistic Revision: The English in this manuscript has been checked by at least two professional editors, both native speakers of English.

NanoLuc Bioluminescence-Driven Photodynamic Activation of Cholecystokinin 1 Receptor with Genetically-Encoded Protein Photosensitizer MiniSOG

In contrast to reversible activation by agonist, cholecystokinin 1 receptor (CCK1R) is permanently activated by singlet oxygen generated in photodynamic action, with sulphonated aluminium phthalocyanine or genetically encoded mini singlet oxygen generator (miniSOG) as photosensitizer. In these works, a halogen light source was used to power photodynamic action. For possible in vivo application of photodynamic CCK1R physiology, bearing a cumbersome light-delivery device connected to an external light source by experimental animals might interfere with their behavior. Therefore, in the present work, the possibility of bioluminescence-driven miniSOG photodynamic CCK1R activation was examined, as monitored by Fura-2 calcium imaging. In parallel experiments, it was found that, after plasma membrane (PM)-localized expression of miniSOGPM in AR4-2J cells, light irradiation with blue light-emitting diode (LED) (450 nm, 85 mW·cm−2, 1.5 min) induced persistent calcium oscillations that were blocked by CCK1R antagonist devazepide 2 nM. NanoLuc was expressed bicistronically with miniSOGPM via an internal ribosome entry site (IRES) sequence (pminiSOGPM-IRES-NanoLuc). The resultant miniSOGPM-IRES-NanoLuc-AR4-2J cells were found to generate strong bioluminescence upon addition of NanoLuc substrate coelenterazine. Strikingly, coelenterazine 5 microM was found to trigger long-lasting calcium oscillations (a hallmark for permanent CCK1R activation) in perifused miniSOGPM-IRES-NanoLuc-AR4-2J cells. These data indicate that NanoLuc bioluminescence can drive miniSOGPM photodynamic CCK1R activation, laying the foundation for its future in vivo applications.