RESEARCH OF ADSORPTION AFLATOXINS BY TECHNICAL LIGNIN

Aflatoxins represent aggressive group of mycotoxins. They are really toxic, carcinogenic and dangerous for human health. There are mechanical, physical and chemical methods for their detoxification. Aflatoxins could be also neutralized by means of various adsorbents as well. We do some research work in the direction of aflatoxin adsorption by presence of lignin, which is quite affordable and gives an effective result.

Fractionation of Cynara cardunculus L. by Acidified Organosolv Treatment for the Extraction of Highly Digestible Cellulose and Technical Lignin

One of the primary targets for the new lignocellulosic feedstock-based biorefinery is the simultaneous valorization of holocellulose and lignin. Acidified organosolv treatment is among the most promising strategy for recovering technical lignin, water-soluble hemicellulose, and cellulose pulp with increased accessibility to hydrolytic enzymes. In this work, a design-of-experiment (DoE) approach was used to increase the cellulose recovery, digestibility, and the delignification of Cynara cardunculus L. feedstock. In the first treatment, the milled biomass was subjected to microwave-assisted extraction using an acidified GVL/water mixture to separate lignin and hemicellulose from cellulose. In the second treatment, the cellulose pulp was hydrolyzed by cellulolytic enzymes to demonstrate the enhanced digestibility. At the optimal condition (154 °C, 2.24% H2SO4, and 0.62 GVL/water ratio), the cellulose pulp showed a cellulose content of 87.59%, while the lignin content was lower than 8%. The cellulose recovery and digestibility were equal to 79.46% and 86.94%, respectively. About 40% of the initial hemicellulose was recovered as monosaccharides. This study demonstrated the effectiveness of the two-step organosolv treatment for biomass fractionation; however, as suggested by DoE analysis, a confirmative study at a low temperature (<154 °C) should be performed to further increase the cellulose recovery.

Fungal Treatment for the Valorization of Technical Soda Lignin

Technical lignins produced as a by-product in biorefinery processes represent a potential source of renewable carbon. In consideration of the possibilities of the industrial transformation of this substrate into various valuable bio-based molecules, the biological deconstruction of a technical soda lignin by filamentous fungi was investigated. The ability of three basidiomycetes (Polyporus brumalis, Pycnoporus sanguineus and Leiotrametes menziesii) to modify this material, the resultant structural and chemical changes, and the secreted proteins during growth on this substrate were investigated. The three fungi could grow on the technical lignin alone, and the growth rate increased when the media were supplemented with glucose or maltose. The proteomic analysis of the culture supernatants after three days of growth revealed the secretion of numerous Carbohydrate-Active Enzymes (CAZymes). The secretomic profiles varied widely between the strains and the presence of technical lignin alone triggered the early secretion of many lignin-acting oxidoreductases. The secretomes were notably rich in glycoside hydrolases and H2O2-producing auxiliary activity enzymes with copper radical oxidases being induced on lignin for all strains. The lignin treatment by fungi modified both the soluble and insoluble lignin fractions. A significant decrease in the amount of soluble higher molar mass compounds was observed in the case of P. sanguineus. This strain was also responsible for the modification of the lower molar mass compounds of the lignin insoluble fraction and a 40% decrease in the thioacidolysis yield. The similarity in the activities of P. sanguineus and P. brumalis in modifying the functional groups of the technical lignin were observed, the results suggest that the lignin has undergone structural changes, or at least changes in its composition, and pave the route for the utilization of filamentous fungi to functionalize technical lignins and produce the enzymes of interest for biorefinery applications.

Electro-oxidative depolymerisation of technical lignin in water using platinum, nickel oxide hydroxide and graphite electrodes

In order to improve the lignin exploitation to added-value bioproducts, a mild chemical conversion route based on electrochemistry was implemented. For the first time, soda lignin Protobind™ 1000 (technical lignin...

Improved understanding of technical lignin functionalization through comprehensive structural characterization of fractionated pine kraft lignins modified by the Mannich reaction

Amino alkylation of technical lignins via the Mannich reaction can be used as an analytical derivatization technique, but also in an applied manner to improve kraft lignin hydrophilicity or metal...