aquatic product
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2021 ◽  
Author(s):  
Zhuo Tian ◽  
Lili Yang ◽  
Xin Qi ◽  
Qiuyue Zheng ◽  
Dejing Shang ◽  
...  

Abstract Background A visualized, rapid, simple method was developed based on loop-mediated isothermal amplification (LAMP) assay to detect Vibrio vulnificus in aquatic products and aquaculture waters.Results Genomic DNA was extracted from Vibrio vulnificus using the boiling and column extraction methods and optimized primers were used to detect the gyrB gene using a visual LAMP method. The sensitivity of the assay was 10 fg/μL, and the results were stable and reliable. Of 655 aquatic product samples and 558 aquaculture waters samples, the positive rates of Vibrio vulnificus detection were 9.01% and 8.60%, respectively, which is markedly higher than that of the traditional culture identification methods. Conclusion The relatively simple technical requirements, low equipment costs, and rapid detection time make the visual LAMP method for detection of Vibrio vulnificus a convenient choice for field diagnosis in the aquaculture industry.


2021 ◽  
Vol 9 ◽  
Author(s):  
Qiongping Sun ◽  
Yonglong Xiong ◽  
Ran Bi ◽  
Xinyi Zhan ◽  
Yueyao Fan ◽  
...  

Per- and polyfluoroalkyl substances (PFASs) have captured worldwide attention due to their persistent and bioaccumulative characteristics. The bullfrog is a highly nutritious and edible aquatic product, but the PFAS accumulation characteristics of bullfrogs had not been paid attention. The present study analyzed the PFAS concentrations in different tissues of cultured bullfrogs and relevant water and sediments collected from the aquaculture region in the coastal area of South China. Results showed that total PFAS concentrations were 50.26–364.25 ng/L in water, with a mean of 135.58 ng/L; concentrations in effluent water were significantly higher than those in influent water, which means bullfrog farming could increase contaminants into the surroundings. The total PFAS concentrations varied from 2.89 to 162.26 ng/g·dw in sediment. The distribution profile of PFASs in sediment was similar to that of water, with short chain playing a significant role. Total PFASs in bullfrog tissues were 3.36–84.07 ng/g·dw, with the highest values in the digestive system (intestines and stomach). As for one novel PFAS alternative, chlorinated polyfluorinated ether sulfonate (F-53B) was found at high detectable levels in all habitat samples and had a higher value of logarithmic bioaccumulation factors. The estimated dietary intake (EDI) for residents in the study area was generally higher than that in other regions; however, the hazard ratios (HRs) for most detected PFASs were far below the safety threshold value “1.” These results suggest that frequent consumption of these bullfrogs would basically not cause severe health effects on local residents in terms of PFASs.


2021 ◽  
Vol 9 (9) ◽  
pp. 1909
Author(s):  
Dandi Hou ◽  
Man Hong ◽  
Yanting Wang ◽  
Pengsheng Dong ◽  
Huangwei Cheng ◽  
...  

As microplastic pollution continues to increase, an emerging threat is the potential for microplastics to act as novel substrates and/or carriers for pathogens. This is of particular concern for aquatic product safety given the growing evidence of microplastic ingestion by aquaculture species. However, the potential risks of pathogens associated with microplastics in mariculture remain poorly understood. Here, an in situ incubation experiment involving three typical microplastics including polyethylene terephthalate (PET), polyethylene (PE), and polypropylene (PP) was conducted during the summer–autumn period in a mariculture cage. The identification of potential pathogens based on the 16S rRNA gene amplicon sequencing and a custom-made database for pathogenic bacteria involved in aquatic environments, was performed to assess the risks of different microplastics attaching potential pathogens. The enrichment of pathogens was not observed in microplastic-associated communities when compared with free-living and particle-attached communities in surrounding seawater. Despite the lower relative abundance, pathogens showed different preferences for three microplastic substrates, of which PET was the most favored by pathogens, especially potentially pathogenic members of Vibrio, Tenacibaculum, and Escherichia. Moreover, the colonization of these pathogens on microplastics was strongly affected by environmental factors (e.g., temperature, nitrite). Our results provide insights into the ecological risks of microplastics in mariculture industry.


Complexity ◽  
2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Yizhuo Zhang ◽  
Fengwei Zhang ◽  
Jinxiang Cheng ◽  
Huan Zhao

With the rapid emergence of the technology of deep learning (DL), it was successfully used in different fields such as the aquatic product. New opportunities in addition to challenges can be created according to this change for helping data processing in the smart fish farm. This study focuses on deep learning applications and how to support different activities in aquatic like identification of the fish, species classification, feeding decision, behavior analysis, estimation size, and prediction of water quality. Power and performance of computing with the analyzed given data are applied in the proposed DL method within fish farming. Results of the proposed method show the significance of contributions in deep learning and how automatic features are extracted. Still, there is a big challenge of using deep learning in an era of artificial intelligence. Training of the proposed method used a large number of labeled images got from the Fish4Knowledge dataset. The proposed method based on suitable feature extracted from the fish achieved good results in terms of recognition rate and accuracy.


2021 ◽  
Vol 26 (3) ◽  
pp. 319-327
Author(s):  
Gita Syahputra ◽  
Hariyatun Hariyatun ◽  
Muhammad Firdaus ◽  
Pugoh Santoso

Sand sea cucumber (Holothuria scabra) is an aquatic product that belongs to Echinodermata, a habitant in almost all Indonesian seas. The main component of the sea cucumber is protein, one of which is collagen. This study aimed to extract and characterize collagen from the species using the acid-base extraction method. The characterization of sea cucumber collagen includes molecular weight, amino acid components, Fourier transform infrared spectrophotometry, and scanning electron microscopy analysis. This study has successfully extracted collagen from the sample using an extraction system: NaOH 0.1 M; CH3COOH 0.1 M; and distilled water under 45°C treatments, gave 6% yield. The collagen has a molecular weight 110-130 kDa. Based on the infrared spectra, the specific functional groups of the collagen are amide A (3379.29 cm-1), amide B (2924.09 cm-1), amide I (1681.93 cm-1), amide II (1560.41 cm-1), and amide III (1249.87 cm-1). The collagen falls into type I. We suggest an alternative resource of collagen from sand sea cucumber, other than poultry and mammals.   Keywords: characterization, collagen, extraction, fishery, sand sea cucumber


2021 ◽  
Vol Volume 14 ◽  
pp. 759-768
Author(s):  
Jiefeng Wang ◽  
Ailian Wang ◽  
Lian Zhu ◽  
Haohan Wang

2021 ◽  
Vol 8 ◽  
Author(s):  
Xin-Yun Wang ◽  
Jing Xie ◽  
Xin-Jun Chen

Aquatic products, such as fish, are popular throughout the world due to their satisfying flavor characteristics as well as rich animal nutrition, and they provide high-value food therapy, but they are easily oxidized and spoiled. It is necessary to detect aquatic products through rapid and accurate technology. Low-field nuclear magnetic resonance (LF-NMR) and magnetic resonance imaging (MRI) have been widely used in the aquatic product industry due to their sensitivity, fast analysis, non-destructive nature and low cost. The applications of LF-NMR in the measurement of aquatic product quality and nutrients (water, fat, and protein) are summarized in this paper. Applications in aquatic products have been shown to depend on deep processing, storage and authentication. This review discusses the application of MRI technology in the quality control of aquatic products. Therefore, this review will guide the application of the aquatic products industry and aims to supply the reader with both the theory of the method and practical applications of the method for use as a rapid and non-destructive technology in scientific research and the industrial industry.


Author(s):  
Yongjun Zhang ◽  
Yanfeng Liu ◽  
Zhang Jiong ◽  
Xiaoshan Zhang ◽  
Baotian Li ◽  
...  

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