Metal Akümülatör Bitki olan Brassica nigra L.’nin in vitro Kallus İndüksiyonu ve Sürgün Gelişimi

This study established an efficient in vitro callus formation and plant regeneration protocol for a prevıously reported Cu accumulator, Brassica nigra, black mustard collected from Diyarbakir (Station site). Node explants from 10-day old mature plants were used for callus formation and shoot regeneration. The highest callus formation frequency (100%) was observed on Murasige Skoog (MS) medium containing 0.1 mg/L Benzylaminopurine (BAP) + 0.5 mg/L Naphthylacetic acid (NAA) (MS 2), 0.6 mg/L BAP + 0.2 mg/L NAA (MS 7), the highest shoot regeneration frequency (100%) was achieved on MS medium containing 0.6 mg/L BAP + 0.05 mg/L Indole butyric acid (IBA) (MS 8), 0.2 mg/L IBA + 0.2 mg/L NAA (MS 10) and the highest number of shoots per explant (3,25) was obtained on MS medium supplemented 0.6 mg/L BAP + 0.05 mg/L IBA (MS 8). After root, stem, and leaf formation from explants in MS medium, these plants were transferred to soil and grown in the plant growth room for one month. A dependable and effective shoot regeneration procedure was developed, laying the groundwork for genetic transformation in Brassica nigra.

EVALUATION OF MINERALIZED PLASMATIC MATRIX DURING SINUS LIFT WITH THE SIMULTANEOUS PLACEMENT OF DENTAL IMPLANTS

Objectives:Mineralized plasmatic matrix is reported to improve the quality of the bone/fibrin mixture, creating a stable and easy to handle homogeneous material. However, few studies evaluate the use of the mineralized plasmatic matrix during sinus lift with the simultaneous placement of dental implants. Purpose: This study evaluated the efficiency or not of MPM compared to the xenograft bone grafting in the maxillary sinus lift. Patients and methods:This study was conducted randomly on patients selected for treatment with a total of sixteen lateral windows sinus lift with simultaneous implant placement. Their ages ranged between 20 and 60 years old. Participating patients were divided into two groups equally and randomly The control group: eightsinus floor elevation was performed using simultaneous implant placement. As a grafting material, Xenograft was used. The study group: eight Sinus floor elevation was achieved with simultaneous implant placement. Xenograft has been used in the form of MPM as a grafting material. CBCT taken before and after sinus augmentation to measure bone volume and height after 1 week of baseline (T0), after 6 months (T1), and after 12 months of baseline (T0) (T2). The Osstell(PT) was used for assessment implant stability at implant insertion (PT0) as well as for loading visit (PT1). Result:A significant difference was observed between the two groups in bone volume (p=0.049). No significant difference was observed between the two groups (p=0.129) in height of graft. Conclusion:MPM eliminated the need for barrier membranes when a guided bone regeneration procedure is considered.The useofMPM as a grafting bone offered greater graft stability and handling.

Esthetic treatment of anterior implants: A case report

Aesthetic implant treatment in the maxillary anterior area can be challenging, especially in case of insufficient bone volume. In this clinical report, a guided bone regeneration procedure using the combination of xenograft and allograft with non-resorbable membrane was applied in the atrophic edentulous alveolar crest to reconstruct a proper implant bed. Moreover, Prosthetic tissue molding was performed to improve success and optimize aesthetics. This article provides information rele- vant to treatment plans, surgical procedures, and prosthetic management in aesthetically important areas.

Method for testing modern common rail piezoelectric fuel injectors

The paper presents the author's own method for testing piezoelectric common rail fuel injectors, which for many years were considered non-repairable components. This was mainly due to the lack of availability of spare parts and dedicated measuring equipment, enabling full diagnostics under test bench conditions. As a result, their workshop and laboratory servicing was very limited, as effective disassembly concerned basicaly only the plunger and barrel assembly (needle with nozzle) for selected reference models. The situation has now improved to such an extent that an author’s own regeneration procedure has been proposed with the replacement of the most important controls and actuators. The tests were carried out on the example of Siemens VDO Continental PCR 2.3 fuel injectors from one engine, listing the most important stages of this process, including the correction of fuel dosage and returns.

PHOSPHORUM SLAG AND PHOSPHOGYPSUM AS MAN-MADE INDUSTRIAL WASTE FOR CREATION OF MODIFIED FIRE AND EXPLOSION-SUPPRESSING POWDER COMPOSITIONS

One of the priority areas of life safety and environmental protection is the creation of modified fire and explosion-suppressing powder compositions using man-made waste from various industries. Their use makes it possible to reuse substandard (expired) fire extinguishing compositions, possibly as substances that slow down the processes of explosion and combustion of materials. Fire and explosion-suppressing powders at the end of the shelf life must be regenerated or disposed of. For the regeneration procedure, i.e. restoration of the fire-extinguishing properties of the powder, it is sent to the manufacturing plant to restore its properties and characteristics. This allows you to modify the properties of the powder and produce an improved product. At the same time, a significant contribution is made to the development of a system for the rational use of natural resources, disposal of production waste, preservation of the ecological situation in the area, as well as prevention of emergencies and emergencies at industrial dumps. Comprehensive and rational use of secondary material and raw materials and wastes of various industries should take a certain place in the raw material balance of industry and in the prevention of emergencies.

Regeneration of Viburnum dentatum L. from Alginate-Encapsulated Shoot Explants after Short-Term Cold Storage and Assessment of Genetic Stability Using ISSR Analysis

The present study demonstrates an efficient protocol for alginate encapsulation, interim cold storing of artificial seeds and conversion to genetically stable plants of Viburnum dentatum L. “Lucidum Aiton”. Explants of shoot tips and first-node segments, excised from in vitro-derived viburnum microshoots, were encapsulated in 2.5% sodium alginate mixed with liquid MS nutrient medium and hardened in 50 mM of calcium chloride producing solid, soft and uniform beads. These artificial seeds achieved 28.9% germination under light, forming 4.3 microshoots per bead. However, with 100 mM of calcium chloride for hardening, the beads were firm and of a uniform globular shape and suitable for handling and exhibited a germination response of 48.9%. Encapsulated shoot tip explants of viburnum, which were stored at 4 °C for 4, 8 or 12 weeks, showed a gradual decline in regeneration response (73.3, 62.2, 51.1%, respectively), while non-encapsulated explants, stored under same conditions, did not survive after the fourth week of cold storage. Microshoots from cold-stored encapsulated explants, which were rooted in solid MS nutrient medium with 0.5 μΜ of Indole-3-acetic acid (IAA) and transplanted to a substrate of peat-perlite (3:1, v/v), acclimatized successfully after application of 75 or 50% shading, which was gradually reduced, and were established with minimum losses in a greenhouse. For the genetic stability of the artificial seed-derived plantlets and compared with the mother plant, an assessment was conducted using Inter Simple Sequence Repeats (ISSRs) analysis. The ISSR profiles proved the genetic uniformity and clonal stability of the regenerated plantlets and their genetic resemblance to the mother plant. The present regeneration procedure could be used as an alternative method for the micropropagation of V. dentatum.

Barrier membranes for guided bone regeneration

The aim of this study was to review the possibilities of using the various types of membranes currently available and used in the guided bone regeneration procedure. Literary sources were analyzed, including English-language articles from the pubmed and elibrary information portals. For horizontal defects, it is most preferable to use resorbable membranes with various modifications of surgical techniques, while for vertical defects, the use of wire-frame non-reabsorbable membranes is most acceptable.

Battery Charging Procedure Proposal Including Regeneration of Short-Circuited and Deeply Discharged LiFePO4 Traction Batteries

The presented paper discusses the most often damages applying for lithium traction and non-traction cells. The focus is therefore given on investigation of possibilities related to the recovery of such damaged lithium-ion batteries, more specifically after long-term short-circuit and deep discharge. For this purpose, initially, the short-circuit was applied to the selected type of traction LiFePO4 cell. Also, the deeply discharged cell was identified and observed. Both damaged cells would exhibit visible damage if electro-mechanical properties were measured. Individual types of damage require a different approach for battery regeneration to recover cells as much as possible. For this purpose, experimental set-up for automated system integrating proposed recovery methods were realized, while battery under test undergone a full-range of regeneration procedure. As a verification of the proposed regeneration algorithms, the test of delivered Ampere-hours (Ah) for various discharging currents was realized both for short-circuited as well as deeply discharged cells. Received results have been compared to the new/referenced cell, which undergoes the same test of delivered Ah. From the final evaluation is seen, that proposed procedure can recover damaged cell up to 80% of its full capacity if short-circuit was applied, or 70% if a deeply discharged cell is considered.