Optimization of the extraction and precipitation process of a leaf protein concentrate from Moringa oleifera Lam.

This study aimed to determine the best extraction and precipitation conditions of Moringa oleifera Lam. leaf protein. The influence of pH (10, 11, 12) and the concentration of NaCl (0, 0.25, 0.5) for the protein extraction process were studied through a Completely Randomized Design (CRD) with factorial arrange 32. The combination of pH 11 and 12 with 0 M NaCl had the best yield (P<0.05). The treatment of pH 11 without NaCl followed a precipitation stage for its purification, and the effect of different levels of pH (4, 4.5, 5) and temperature (40, 60, 80 °C) were evaluated using a CRD with factorial arrange 22 and 6 central points. The temperature did not affect the yield of the process in a significant way and the amount of precipitate was maximized at pH 4 and 4.5. From 100 g of the dry leaf, 7.26±0.19 g of protein was isolated with a recovery of 26.93±0.22 g 100 g-1 from the total protein. Due to their astringency and bitterness, consuming large amounts of Moringa oleifera Lam leaves is not a solution; therefore, obtaining a leaf proteinconcentrate could be useful for diverse applications in nutritional supplements, and as raw material for functional products development.

Optimization of foam mat drying process of moringa leaf powder (Moringa oleifera) as protein and amino acids sources

Moringa (Moringa oleifera) is a well famous plant that grows in almost all parts of Indonesia. These plants have many benefits in the fields of food, cosmetics, and health. The components of moringa that can be utilized are the nutrients and the bioactive compounds. One of the nutrients that are required by the human body is protein. This protein can be taken through the extraction method, but unfortunately, the extract has several drawbacks because it is easily rotten. As a result, further methods are needed to maintain the shelf life and product quality, namely microencapsulation using foam mat drying method. The present study was intended to obtain the optimum concentration of maltodextrin, tween 80, and drying temperature in microencapsulation using the foam mat drying method so that the protein and amino acids in Moringa leaf protein concentrate powder can be protected. Besides, it also was aimed at determining the digestibility of the Moringa leaf protein concentrate powder. This study consisted of three factors, namely X1 (Tween 80 concentration) which contained three levels, namely 0.1, 0.2, 03%; X2 (maltodextrin concentration) which had three levels, namely 5, 10, 15%; and X3 (foam mat drying temperature) consisting three levels, namely 50, 55, 60°C which were arranged using the Response Surface Method (RSM) with the Central Composite Design (CCD). The research parameters utilized were total yield, total protein, amino acid content, and protein digestibility. The results of the drying process optimization were tween 80 concentrations of 0.201%, maltodextrin up to 13.79%, and the temperature was 53.46°C with a total yield of 25.17% and a protein content of 25.377% with the desirability of 0.846. The combination of these treatments also produces 20 amino acid components in Moringa leaf powder and the protein digestibility is quite high, namely 62, 856%.

Identification of Bioactive Phytochemicals in Leaf Protein Concentrate of Jerusalem Artichoke (Helianthus tuberosus L.)

Jerusalem artichoke (JA) is widely known to have inulin-rich tubers. However, its fresh aerial biomass produces significant levels of leaf protein and economic bioactive phytochemicals. We have characterized leaf protein concentrate (JAPC) isolated from green biomass of three Jerusalem artichoke clones, Alba, Fuseau, and Kalevala, and its nutritional value for the human diet or animal feeding. The JAPC yield varied from 28.6 to 31.2 g DM kg−1 green biomass with an average total protein content of 33.3% on a dry mass basis. The qualitative analysis of the phytochemical composition of JAPC was performed by ultra-high performance liquid chromatography-electrospray ionization-Orbitrap/mass spectrometry analysis (UHPLC-ESI-ORBITRAP-MS/MS). Fifty-three phytochemicals were successfully identified in JAPC. In addition to the phenolic acids (especially mono- and di-hydroxycinnamic acid esters of quinic acids) several medically important hydroxylated methoxyflavones, i.e., dimethoxy-tetrahydroxyflavone, dihydroxy-methoxyflavone, hymenoxin, and nevadensin, were detected in the JAPC for the first time. Liquiritigenin, an estrogenic-like flavanone, was measured in the JAPC as well as butein and kukulkanin B, as chalcones. The results also showed high contents of the essential amino acids and polyunsaturated fatty acids (PUFAs; 66-68%) in JAPC. Linolenic acid represented 39–43% of the total lipid content; moreover, the ratio between ω-6 and ω-3 fatty acids in the JAPC was ~0.6:1. Comparing the JA clones, no major differences in phytochemicals, fatty acid, or amino acid compositions were observed. This paper confirms the economic and nutritional value of JAPC as it is not only an alternative plant protein source but also as a good source of biological valuable phytochemicals.

High Modernism and the Development Decade

This chapter concerns the period of high modernism, when expansive ambitions of scientific progress promised new foods from a variety of fantastical sources, including algae grown on sewage, fungi grown on oil, and inedible leaves that could be boiled into protein curd. These projects encapsulated the vision of a cheap, efficient, mass-produced famine treatment that could finally end starvation. Beginning with Leaf Protein Concentrate, which emerged from postwar investigations into food shortage, high modernist hopes soon moved to less appetizing foods made from algae and junk fish, driven by an even more futuristic and top-down vision of life emerging from primordial sources. After this initial burst of enthusiasm came a different high modernist concept: the complete nutritional wonder-product, which was meant to offer a balanced meal in a simple powder or sachet. After generating great interest in aid agencies, this ideology began to go into decline by the early 1970s, limited by expense, complexity, and fundamental impracticality.

Finding valuable bioactive components from Jerusalem artichoke (Helianthus tuberosus L.) leaf protein concentrate in a green biorefinery concept

Jerusalem artichoke is widely known for its inulin-enriched tubers. Recently the opportunity has been arisen to involve the whole plant in biorefinery concept due to its high lignocellulose biomass and tuber production. This paper focuses on the repeatedly harvestable green biomass of Jerusalem artichoke. Ultra-High Performance Liquid Chromatography-Electrospray Ionization/Mass Spectrometry (UHPLC-ESI-MS) was applied to identify the phytochemicals in Jerusalem artichoke leaf protein concentrate (JAPC) thermally extracted from green biomass of three clones, i.e., Alba, Fuseau and Kalevala. Amino acid and fatty acid profiles as well as yield of JAPC were also analyzed. The UHPLC-ESI-MS analyses showed that no toxic phytochemicals were identified in JAPC. The results revealed, also, that JAPC is not only essential-amino acids-rich but also contains substantial amounts of polyunsaturated fatty acids (66-68%) such as linolenic and linoleic acids. Linolenic acid represented 39-43% of total lipid content; moreover, the ratio between ω-6 and ω-3 essential fatty acids in JAPC was ∼0.6: 1. Using UHPLC-ESI-MS, the following hydroxylated methoxyflavones were for the first time identified in JAPC, i.e., dimethoxy-tetrahydroxyflavone, dihydroxy-methoxyflavone, hymenoxin and nevadensin. These compounds are medically important since they are referred to as anti-cancer, anti-inflammatory and antioxidants. Also, liquiritigenin - estrogenic-like compound - was identified in JAPC alongside the following terpenes, i,e., loliolide and dihydroactinidiolide. However, no remarkable differences of phytochemicals, fatty acids and amino acids composition were seen among Jerusalem artichoke clones. The green biomass of tested clones ranged between 5 to 5.6 kg m-2 and JAPC yield varied from 28.6 to 31.2 g DM kg-1 green biomass with total protein content, on average, of 33.3%. According to our knowledge, this paper is the first scientific report highlighting bioactive substances in JAPC such as PUFA phytochemicals. These results clearly prove that JAPC is a valuable product which can direct towards human and animal nutrition as well as it can serve as basic material for different industrial purposes.