Biochemical and cytological features of onion bulbs and leaves collected from various ecogeographical origins

Against the background of global climate change, drought stress has become one of the environmental limiting factors that can significantly influence the growth and development of crop plants. Drought stress conditions also cause changes in plant physiological and metabolic processes. The influence of soil drought on the mineral composition of the leaves of two Actinidia species with С3-type photosynthesis, namely, Actinidia argutа (Siebold & Zucc.) Planch. ex Miq. cultivar ‘Taezhny Dar’ and Actinidia kolomikta (Maxim. & Rupr.) Maxim. cultivar ‘Narodnaya’, was studied through energy dispersive spectrometry. The investigations were carried out during 2020 to 2021 at the Department of Genofonde and Bioresources of Plants, Federal Scientific Center for Horticulture, Moscow. The present research revealed that actinidia leaves contained the following major elements: K (11.19 mass% to 13.84 mass%), Ca (7.83% to 12.08 mass%), Cl (6.20 mass% to 7.33 mass%), and Mg (2.98 mass% to 3.44 mass%). Low values were recorded for Mo (1.19 mass% to 4.49 mass%) and P (0.83 mass% to 1.25 mass%). In both species, the mineral elements K and Ca were present at high levels. A positive correlation was observed between Mg–P, K–Mn, Mn–Se, Cu-Se, P–Si, Na–Mo, and Si–Mn in the leaves of A. argutа and between Cl–Ca, Mo; P–Si, Mo; and K–Ca in the leaves of A. kolomikta. Under stress conditions, the ratios of K/Ca and K/P declined to 0.9 and 6.3, respectively, whereas those of K/Cl, K/Mg, and K/Mo increased to 3.8, 4.4, and 2.7, respectively. The present studies confirmed that actinidia leaves contained high concentrations of minerals, especially K, Ca, P, and Mg, and that the accumulation of mineral elements in actinidia plant leaves under drought conditions varied depending on the species.

Primary Small Cell Malignancies of the Breast: Are They Rare Malignancies?

In contrast with the other organs such as the lung, small cell tumors have been less studied in the breast due to their relatively less frequency. Although rare, neuroendocrine neoplasms, some lymphomas, and some small cell sarcomas such as undifferentiated small round cell sarcoma and rhabdomyosarcoma can be seen in small cell morphology in the breast. Many cytological specimens such as fine-needle aspiration biopsies and touch imprint cytology are used for diagnosis and further prognostic/predictive marker determination in primary breast masses, sentinel and axillary lymph nodes, and metastatic masses. Lobular carcinoma deserves to be considered in the small cell tumor group because of its small, monomorphic, discohesive, scant cytoplasmic cytological features. Since so many different types of tumors in the breast can have small cell characteristics, they should be divided into small cell neuroendocrine tumors and small cell nonneuroendocrine tumors. When evaluating small cell breast tumors cytologically, wide tumor diversity should be kept in mind, and clinical, hematological, and radiological features should be taken into consideration.

Clinical and Cytology Description of Various Neck Lumps in Madani Medan Hospital

This study aims to determine the clinical and cytological features of various necklumps. This study used a descriptive observational method with a cross-sectionalapproach to determine the clinical and cytological features of various neck lumps.This study used secondary data of patients with indications of a neck lump whowere undergoing treatment or examination at the Medan Madani Hospital in 2018- 2019. The population in this study were all patients with symptoms and positivesexperiencing a lump in the neck, at Madani Hospital Medan. Data processing iscarried out after the observation and recording of medical record data is inaccordance with what is needed, the data obtained is tabulated and coded for lateranalysis. The results of this study indicated that respondents who experiencedthyroid nodules were more dominant in female respondents with a total of 11respondents, with the most common type of thyroid nodule adenoma follicularthyroid, namely 9 respondents with female gender. Respondents who experiencedinflammation were dominated by female respondents with a non-specific chronicinflammation process on the right side of the neck with a total of 10 respondentswith a percentage of 23.8%.

Germinal Matrix -Intraventricular Hemorrhage: Current Concepts and Future Direction

Germinal Matrix Hemorrhage -Intraventricular hemorrhage (IVH) is a bleed of multifactorial etiology involving the highly vascular and delicate neuro-glial precursors in the developing brain. It poses a challenging complication in preterm newborns. This chapter provides a focused discussion on the current concepts in pathogenesis, management, and complications of IVH. The radiological findings at diagnosis and follow-up and the cytological features of CSF will be valuable to both frontline and diagnostic healthcare providers. The chapter also reviews the ongoing scientific development in the field. The authors believe that this chapter will be a valuable tool for all healthcare providers (students, physicians, and in nursing care) in managing this challenging condition.

The Rapid Cytological Process of Grain Determines Early Maturity in Weedy Rice

Shorter grain-filling period and rapid endosperm development endow weedy rice (WR) with early maturity compared to cultivated rice (CR). However, the role of the cytological features and antioxidative enzyme system during grain development are largely unexplored. We selected four biotypes of WR and their associated cultivated rice (ACR) types from different latitudes to conduct a common garden experiment. The difference in the cytological features of endosperm between WR and ACR was compared by chemical staining, and the cell viability and nuclear morphometry of endosperm cells were observed by optical microscopy. Furthermore, antioxidative enzyme activity was measured during grain filling. Anatomic observation of endosperm shows that the development process of endosperm cell in WR was more rapid and earlier than that in ACR. The percentage of degraded nuclei of WR was 2–83% more than that of ACR. Endosperm cells in WR lost viability 2–6 days earlier than those in ACR. The antioxidant enzyme activity of WR was lower than that of ACR during grain filling. The ability of WR to scavenge reactive oxygen species (ROS) was weaker than that of ACR, which may contribute to the rapid cytological process in the endosperm cells of WR. The rapid cytological process and weaker ability to scavenge ROS in endosperm cells may contribute to early maturity in WR.

Idiopathic Thrombocytopenic Purpura in Post COVID-19 Condition: Case Study

A 50 years old woman was referred to hospital with complaints of bluish black discoloration of skin or multiple ecchymosis since 6 days and skin rashes, pink changes or petechiae changes since 1 week, dark color stools since 3 days. She had a history of COVID-19 positive on past ‘‘3 months’’ back and she received the corticosteroids, antiviral drugs, broad spectrum antibiotics, anticoagulants, and vitamin B and C supplements. Now patient is admitted and investigated for further management. Her bone marrow examination reveals marrow cytological features are compatible with immune thrombocytopenic Purpura and peripheral examination reveals red cells are microcytic hypochromic with elongation forms, platelets are markedly reduced and elevation of CRP, reduction of the Hb, PCV, MCH, MCV, MCHC, APTT, Serum vitamin B12, Lymphocytes and her HRCT-Chest shows CORADS 5, Rapid antigen test shows positive. A review on introduction of the disease, etiology, pathogenesis, pathophysiology, mechanism of thrombocytopenia in COVID-19 patients and grading is explained in literature review.

Cytopathological and histopathological evaluation of neck mass in a tertiary care hospital

<p class="abstract"><strong>Background:</strong> Patients with neck swellings are commonly seen in ENT outpatient and leads to dilemma in diagnosis. To prevent unnecessary investigations and surgery a simple and sensitive diagnostic tool is needed. Fine needle aspiration cytology (FNAC) is a simple and sensitive diagnostic tool that can provide results in minutes.</p><p class="abstract"><strong>Methods:</strong> This prospective study was done at the department of ENT, Mandya institute of medical sciences, Mandya, Karnataka, India from November 2017 to April 2019 including 100 cases of neck masses in patients aged above 18 years. FNAC was done for all neck masses and then these cases were subjected for biopsy. The cytological features was then reviewed with corresponding histopathology features.</p><p class="abstract"><strong>Results:</strong> Out of the 100 neck masses under study 67 (67%) were males and 33 (33%) were females with male:female ratio (1:2.03). Thyroid aspirations (43%) were most common followed by lymph node (24%), salivary gland aspirations (18%), congenital swellings (8%) and others (7%). Out of the 100 cases 26% were neoplastic and 74% were non-neoplastic. Histopathological correlations were available in all the 100 cases with sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of 84.2%, 98.65%, 95.65% and 94.81% respectively. FNAC was in correlation with histopathology in 86% of cases and found to be statistically significant.</p><p class="abstract"><strong>Conclusions:</strong> FNAC is safe, simple and minimally invasive first line investigation of choice for the patients presenting with palpable neck masses and can provide results rapidly and but histopathology remains the gold standard.</p>

Seborrheic Keratosis-Like Lesion of the Cervix: First Report of the Cytological Features of a Low-Risk HPV 42-Associated Lesion

<b><i>Introduction:</i></b> Seborrheic keratosis-like lesion of the cervix and vagina is a rare lesion and shows similar morphology to vulvar seborrheic keratosis; 3 of the 7 previously reported cases were associated with low-risk human papillomavirus (HPV) type 42. We report a case of seborrheic keratosis-like lesion of the cervix and provide the first description of the cytological features of this lesion. <b><i>Case Presentation:</i></b> A woman in her late forties presented with postcoital bleeding. She had a cervical screening test following which she underwent cervical biopsy, endocervical and endometrial curettage, large loop excision of the transformation zone of the cervix, and hysterectomy. <b><i>Results:</i></b> The liquid-based cytology preparation showed cohesive groups of mildly atypical squamoid cells with a spindle cell morphology, mildly increased nuclear to cytoplasmic ratio, prominent nucleoli, and occasional nuclear grooves. No koilocytes were identified. Molecular genotyping revealed positivity for HPV type 42. <b><i>Discussion/Conclusion:</i></b> This represents the first description of the cytological features of a seborrheic keratosis-like lesion of the cervix, which are distinctive and unusual. Whilst the mild squamous atypia raised the possibility of a low-grade squamous intraepithelial lesion, no koilocytes were identified. The association in our case with a low-risk HPV type, HPV 42, provides further evidence for a role of this HPV type in the pathogenesis of these lesions.