Cellulose Microfiber Encapsulated Probiotic: Viability, Acid and Bile Tolerance during Storage at Different Temperature

This work aimed to analyze the physicochemical properties of cellulose from OPT used in the fabrication of CMF and evaluate the efficacy of the hydrogel CMF as an encapsulant for L. fermentum InaCC B1295 stored at room temperature and in the refrigerator. The Kjeldahl method was used to evaluate the protein content; the gravimetric method was used to determine OPT's ash, moisture, and fiber contents; the Soxhlet method was used to determine the fat content carbohydrates were computed using the difference method. The levels of holocellulose, lignin, and cellulose were also determined. Viability, acid and bile resistance of strain B1295 were evaluated at various temperatures for 35 days. The most abundant component of OPT fiber was cellulose, followed by hemicellulose and lignin. XRD examination revealed that OPT cellulose has a crystal index of 83.40%. FTIR analysis was used to detect the stretching vibrations of the –OH group on cellulose at 3419.03 cm-1. CMF hydrogel from OPT sustained L. fermentum InaCC B1295 survival for up to 28 days at room and refrigerated temperatures. At acidic conditions and in the presence of bile, the viability of L. fermentum InaCC B1295 was excellent, with a drop in cell population of less than 0.2 log CFU/g over 35 days at room and refrigerated temperatures. CMF obtained from OPT can be used as an encapsulant to maintain viability, acid resistance and bile of probiotics. There is still a need for research into the usage of CMF from OPT in combination with other encapsulants to extend the storage life of L. fermentum InaCC B1295. Doi: 10.28991/ESJ-2022-06-01-08 Full Text: PDF

Establishment of metrological parameters of the method for measuring the protein mass fraction in fish food products by the Kjeldahl method

In laboratory practice, there are many protein quantification methods, and all of them have their own advantages and disadvantages. The most common and widely used method for the protein analysis in food products, including fish, is the Kjeldahl method. However, the current standards for measurement methods for the determination of the protein content in fish food products do not provide for the use of devices that meet the modern level of technical development, and also do not contain metrological indicators that guarantee the reliability of the results obtained. The aim of the study was to substantiate the method for measuring the protein mass fraction in fish food products by the Kjeldahl method on an automatic analyzer and to establish metrological parameters. The assessment of the quality indicators of the Kjeldahl measuring method was carried out using a Kjeltec System 2300 Nitrogen Analyzer (Foss Analytical AB, Sweden) in the form of a characteristic of the measurement error and its components, which will provide results with the required accuracy.

Evolution of in vitro digestibility techniques: a systematic review

The inability to reproduce certain digestive processes in vivo, high research costs and ethical aspects have led to the development of a large number of in vitro digestion models. These models allow us to take into account various factors of modeling complex multistage physiological processes occurring in the gastrointestinal tract, which makes them promising and widely used. A significant part of in vitro methods includes assessment by enzymatic digestion and are based on the calculation of nitrogen remaining after digestion in relation to the initial total nitrogen (according to the Dumas, Kjeldahl method, spectrophotometric or chromatographic method). There are also a number of titrometric methods (pH‑stat), which are mainly used to assess the digestibility of feed, most successfully for aquatic animals due to the simplicity of their digestive tract. Methods for assessing the digestibility of food products by enzymatic digestion have undergone various stages of evolution (since 1947) and have been widely modified by including various enzymes (pepsin, trypsin, pancreatin, erepsin, etc.) in model systems, indices for various products have been determined on their basis (pepsin-digest-residue (PDR) index, 1956; pepsin pancreatin digest (PPD) index, 1964; pepsin digest dialysate (PDD), 1989). As a result, a single protocol was formed to study the digestibility of food — INFOGEST (2014–2019), which includes three stages of digestion (oral, gastric and intestinal). It allows researchers to accurately reproduce the conditions of the human gastrointestinal tract and is widely used by scientists around the world.

Molecular laser-induced breakdown spectroscopy technique for the detection of nitrogen in waters

Nitrogen (N) content is a significant indicator for use in evaluating eutrophication. However, the conventional method, such as alkaline potassium persulfate digestion ultraviolet (UV) spectrophotometric or Kjeldahl method, always require...

Effects of Indigenous and Non-Indigenous Arbuscular Mycorrhizal Fungi on Growth and Plant Nutrient Uptake by Terung Asam (Solanum lasiocarpum Dunal)

Terung asam (Solanum lasiocarpum Dunal) is a native fruit vegetable that is gaining interest as a commercial crop in Sarawak and Malaysia. Malaysia is covered by soils that are highly weathered, acidic, and low in fertility and depend on chemical fertilisers to promote good plant growth. Alternative means to reduce dependency on chemical fertiliser, for example arbuscular mycorrhiza fungi (AMF), must be sourced. Very few research on terung asam has been documented particularly on nutrients uptake. The objectives of this research were to investigate the effect of indigenous and non-indigenous AMF on nutrient uptake by terung asam. A greenhouse experiment was conducted consisting of three treatments namely control (T1), indigenous AMF (T2), non-indigenous AMF (T3). The treatments were arranged in a complete randomised design with four subsamples and four replicates. Thirty-day-old seedlings were transplanted and measured for their heights and stem diameters for 90 days. Fresh and dry shoot and root weights were taken during harvesting. Plant nutrient analyses were conducted using Kjeldahl method for total N, single ashing for P and single ashing and double acid for K, Ca and Mg. The results revealed the addition of AMF spores at 200 spores per pot, increased plant height by 13 to 33% and stem diameter by 5 to 25% and more leaves were retained by T3 plants at harvesting. T3 recorded higher fresh shoot (11.27%) and dry shoot (14.98%) as well as fresh root (23.67%) and dry root (22.77%) weights than T1 plants. Addition of AMF in treatments T2 and T3 promoted better nutrient uptake by aboveground and belowground biomasses particularly for K, Ca and Mg. T3 was superior in terms of the nutrient uptake for most nutrients. AMF used in T3 showed better results as the AMF spores were proven effective in promoting plant growth while AMF used in T2 were obtained from the field and untested. The findings of this study showed the potential of indigenous and non-indigenous AMF in promoting growth and nutrient uptake by terung asam plants.

Pembuatan Konsentrat Protein Ampas Kelapa (Cocos nucifera L.) Bebas Lemak pada Berbagai Konsentrasi NaOH

Coconut pulp contains up to 18.2% protein which is one of the wastes from coconut oil processing. Protein from coconut pulp can be used in the manufacture of protein concentrates which are widely used in the food industry. The purpose of this research is to determine the concentration of NaOH that can produce the yield and protein content of coconut pulp protein concentrate. Coconut pulp protein was extracted with NaOH at various concentrations of 0.5, 1, 1.5, 2, and 2.5 M, then continued with salting out using salt (NH4)2SO4 with a saturation of 65%. The crude protein content of coconut pulp protein concentrate was determined by the Kjeldahl method. The results showed that the use of 0.5 M NaOH resulted in the highest protein content of coconut pulp protein concentrate, which was 71.30% with a yield of 14.42%.

The content of basic elements in buckwheat flour as affected by varietal characteristics

- Purpose. Determine the biochemical composition of buckwheat as affected by varietal characteristics. Methods. Humidity was determined by thermogravimetric method, protein content by the Kjeldahl method, carbohydrate content by sugar meter, ash content by burning in a muffle furnace, fat content by low-fat residue, vitamin content by liquid chromatography. Results. The biochemical composition of buckwheat grain varied significantly depending on the variety. Studies have shown that at a moisture content of 11.0–12.6%, the content of fiber, organic acids, ash, mono- and disaccharides and fat was the lowest. Thus, their content over varieties was 0.5–2.5%, 1.7–2.4, 1.5–2.5, 1.6–2.5 and 2.0–3.2%, respectively. Of the 17 varieties of buckwheat, only 4 had a significantly lower protein content (13.2–14.0%) compared to the standard variety ‘Ukrainka’. In other varieties, this figure varied from 16.3 to 18.5% or more, that is by 9–23% compared to the control. Buckwheat had the highest starch content of 57.2–62.0% depending on the variety. It should be noted that no significant difference was found between tall and dwarf varieties. Thus, the protein content in grain obtained from dwarf varieties varied from 16.5 to 17.2% while in tall ones from 13.2 to 18.5%. The content of vitamins in grain also varied depending on the variety from which it was obtained. The content of vitamin K was the lowest, 0.005–0.007 mg/100 g, the content of vitamins B2, B1, B5 and B6 varied from 0.12 to 0.42 mg/100 g of grain. The content of vitamin E and B3 was higher, 3.00–6.56 mg/100 g of grain, depending on the variety. The highest content of B4 was 50.0–54.3 mg/100 g of grain. It should be noted that the share of vitamin K in the total amount was the lowest (0.01%), the share of vitamins B2, B1, B5 and B6 ranged between 0.2 and 0.7%, E and B3 between 6.1 and 10.3%, and vitamin B4 between 80.3 and 85.9%. According to the content of vitamins, the difference between buckwheat varieties has not been found. The level of vitamins provides a determination of the integral rate of the product. It was found that 100 g of buckwheat provides the adult body with vitamin K by 4–7%, B5 – by 6–9, B4 – by 10–11, B2 – by 11–24, B1 – by 11–36, B6 – by 12–23, E – by 27–44, B3 – by 21–44% depending on the variety. Conclusions. As a result of research, it was found that the biochemical composition of grain varies significantly depending on the buckwheat variety. Buckwheat contains the most vitamins E (3.00–6.63 mg/100 g of cereal) and B4 (51.0–54.3 mg/100 g of cereal). It is determined that grain obtained from ‘Orlovskyi’, ‘Podilskyi’, ‘Yana’, ‘Sumchanka’, ‘Anisia’, ‘Krupnozelena’, ‘Skorostyhla 81’ and ‘Kvitnyk’ varieties have a high content of vitamins B2, B1, B6, E and B3 with an integrated rate of 16–44%. In addition, grain of these varieties contains 16.5–17.4% of protein.

One-Dimensional Convolutional Neural Networks for Hyperspectral Analysis of Nitrogen in Plant Leaves

Accurately determining the nutritional status of plants can prevent many diseases caused by fertilizer disorders. Leaf analysis is one of the most used methods for this purpose. However, in order to get a more accurate result, disorders must be identified before symptoms appear. Therefore, this study aims to identify leaves with excessive nitrogen using one-dimensional convolutional neural networks (1D-CNN) on a dataset of spectral data using the Keras library. Seeds of cucumber were planted in several pots and, after growing the plants, they were divided into different classes of control (without excess nitrogen), N30% (excess application of nitrogen fertilizer by 30%), N60% (60% overdose), and N90% (90% overdose). Hyperspectral data of the samples in the 400–1100 nm range were captured using a hyperspectral camera. The actual amount of nitrogen for each leaf was measured using the Kjeldahl method. Since there were statistically significant differences between the classes, an individual prediction model was designed for each class based on the 1D-CNN algorithm. The main innovation of the present research resides in the application of separate prediction models for each class, and the design of the proposed 1D-CNN regression model. The results showed that the coefficient of determination and the mean squared error for the classes N30%, N60% and N90% were 0.962, 0.0005; 0.968, 0.0003; and 0.967, 0.0007, respectively. Therefore, the proposed method can be effectively used to detect over-application of nitrogen fertilizers in plants.

DEVELOPMENT OF TECHNOLOGY FOR OBTAINING MULTIPLE EMULSION OF OVARIAN LIQUID (CAVIAR SOL), ENSURING THE DURATION OF ITS STORAGE

Дефицит пищевого белка обусловливает актуальность развития отечественного производства пищевых белковых продуктов из биоресурсов вторичного происхождения. Овариальная жидкость (икорный золь) – вторичный продукт икорного производства – может быть использована как физиологически функциональный ингредиент для пищевых продуктов сбалансированного питания. Применяемые способы сохранности овариальной жидкости (ОЖ) приводят к изменению нативных свойств ее компонентов за счет температурного или химического воздействия. Высказано предположение, что капсулирование во множественную эмульсию ОЖ позволит сохранить ее нативные свойства и увеличить срок хранения. Цель настоящего исследования – разработка технологии получения множественной эмульсии ОЖ и установление эффективности новой формы продукта при хранении. Объектом исследования была ОЖ, полученная при заборе икры осетровых рыб с применением метода прижизненного получения. Множественную эмульсию готовили двухступенчатым способом. На первом этапе получали стандартную эмульсию вода–масло, которую затем использовали в качестве одной из фаз для множественной эмульсии вода–масло–ОЖ. Полученный продукт и контрольный образец (ОЖ, прошедшая только фильтрацию) размещали в чашках Петри и хранили в термостатируемой холодильной камере при температуре не ниже 4°С в течение 40 сут. Через каждые 5 сут в отобранных пробах определяли по методу Кьельдаля содержание белковых веществ, небелкового азота и азота летучих оснований. Установлено, что при хранении в ОЖ, прошедшей только фильтрацию, белок разрушался быстрее и его содержание через 40 сут снизилось на 34%, тогда как в ОЖ, заключенной во множественную эмульсию, этот показатель снизился только на 5,9%, что коррелирует со скоростью накопления продуктов распада белков в ОЖ после 40 сут хранения. Предложенный способ получения множественной эмульсии ОЖ позволяет обеспечить сохранность ее белкового комплекса в процессе хранения и разработать новый ассортимент физиологически функциональных продуктов с использованием отечественного биологически ценного пищевого ингредиента. The shortage of dietary protein determines the relevance of the development of domestic production of food protein products from bioresources of secondary origin. Ovarian liquid (caviar ash), or caviar sol, – a secondary product of caviar production – can be used as a physiologically functional ingredient for balanced nutrition foods. The applied methods of preservation of ovarian fluid (OF) lead to a change in the native properties of its components due to temperature or chemical exposure. It is suggested that encapsulation in a multiple emulsion of OF will preserve its native properties and increase the shelf life. The purpose of this study is to develop a technology for obtaining multiple OF emulsion and to establish the effectiveness of a new product form during storage. The object of the study was the OF obtained by sampling the caviar of sturgeon fish using the method of lifetime production. The multiple emulsion was prepared in a two-stage way. At the first stage a standard water–oil emulsion was obtained, which was then used as one of the phases for a multiple water–oil–OF emulsion. The resulting product and the control sample (OF that passed only filtration) were placed in Petri dishes and stored in a thermostatically controlled refrigerator at a temperature of at least 4°C for 40 days. Every 5 days the content of protein substances, non-protein nitrogen and nitrogen of volatile bases was determined in the selected samples by the Kjeldahl method. It was found that when stored in the OF that passed only filtration, the protein was destroyed faster and its content decreased by 34% after 40 days, whereas in the OF enclosed in a multiple emulsion, this indicator decreased by only 5.9%, which correlates with the rate of accumulation of protein breakdown products in the OF after 40 days of storage. The proposed method for obtaining a multiple emulsion of COOLANT makes it possible to ensure the safety of its protein complex during storage and to develop a new range of physiologically functional products using a domestic biologically valuable food ingredient.