hyaluronidase inhibitor
Recently Published Documents


TOTAL DOCUMENTS

59
(FIVE YEARS 2)

H-INDEX

12
(FIVE YEARS 0)

2020 ◽  
Author(s):  
G Pilco ◽  
D Vinueza ◽  
K Acosta ◽  
A Torres

Ecuador se ubica en una zona geográfica privilegiada, presenta zonas climáticas bien definidas con características ambientales y geográficas únicas. Estas particularidades hacen que cada región posea sus propios ecosistemas, existiendo así muchas especies vegetales y animales sin investigar. La población hace uso de estos recursos para tratar diversas dolencias como por ejemplo, Piper peltatum utilizada en caso de mordedura de serpiente. El objetivo del estudio fue determinar si los extractos de esta especie presentaban efectos inhibitorios sobre la hialuronidasa, para lo cual se identificó los metabolitos secundarios presentes mediante el tamizaje fitoquímico, se cuantificó la cantidad de fenoles y flavonoides totales a través de métodos espectrofotométricos y se determinó el porcentaje de inhibición tanto sobre la hialuronidasa bovina como la hialuronidasa presente en el veneno de Naja naja atra y Botrox atrox. Se identificó en P. peltatum alcaloides con potencial actividad antimitótica, cumarinas, terpenos, compuestos fenólicos y flavonoides. La cantidad de fenoles totales fue de 14,49±1,2 mg equivalente de ácido gálico/g extracto seco y 14,53±0,208 mg equivalente de Quercetina/g extracto seco de flavonoides. Respecto a la actividad inhibitoria se concluyó que existe mayor inhibición sobre la hialuronidasa bovina, seguida del veneno de Naja naja atra y no de manera significativa sobre B. atrox. Ecuador is located in a privileged geographical area, it has strongly defined climatic zones with unique environmental and geographical characteristics. These particularities mean that each region has its own ecosystems, so many plant and animal species exist without investigating. The population makes use of these resources to treat various ailments such as Piper peltatum used in case of snakebite. The objective of the study was to determine if extracts of this species has inhibitory effects on hyaluronidase. The secondary metabolites present were identified by phytochemical screening, the amount of phenols and total flavonoids was quantified by spectrophotometric methods, as well as the determination of the inhibition of bovine, Naja naja atra venom and Botrox atrox hyaluronidase. P. peltatum contains alkaloids with potential antimitotic activity, coumarins, terpenes, phenolic compounds and flavonoids. The amount of total phenols was 14.49 ± 1.2 mg gallic acid equivalent / g dry extract and 14.53 ± 0.208 mg Quercetin equivalent /g dry flavonoid extract. Regarding the inhibitory activity, it was concluded that there is greater inhibition on bovine hyaluronidase, followed by Naja naja atra venom and not significantly on B. atrox. Palabras claves: Piper peltatum, hialuronidasa, Naja naja atra, Extracto, Bothrops atrox. Keywords: Piper peltatum, hialuronidase, Naja naja atra, extract, Bothrops atrox.


2016 ◽  
Vol 195 (4S) ◽  
Author(s):  
Martin J.P. Hennig ◽  
Soum D. Lokeshwar ◽  
Shenelle N. Wilson ◽  
Andre R. Jordan ◽  
Juan Chipollini ◽  
...  

Scientifica ◽  
2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Hua-jin Zeng ◽  
Ran Yang ◽  
Jing You ◽  
Ling-bo Qu ◽  
Yan-jun Sun

The inhibitory effect of liquiritigenin on hyaluronidase and its binding mechanism were investigated systematically by UV-vis absorption, fluorescence, and molecular modeling approaches. These results indicated that liquiritigenin could interact with hyaluronidase to form a liquiritigenin-hyaluronidase complex. The binding constant, number of binding sites, and thermodynamic parameters were calculated, which indicated that liquiritigenin could spontaneously bind with hyaluronidase mainly through electrostatic and hydrophobic interactions with one binding site. Synchronous fluorescence, three-dimensional fluorescence, and molecular docking results revealed that liquiritigenin bound directly to the enzyme cavity site and this binding influenced the microenvironment of the hyaluronidase activity site, resulting in reduced hyaluronidase activity. The present study provides useful information for clinical applications of liquiritigenin as a hyaluronidase inhibitor.


2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Shiro Kohi ◽  
Norihiro Sato ◽  
Atsuhiro Koga ◽  
Keiji Hirata ◽  
Enjuro Harunari ◽  
...  

Pancreatic ductal adenocarcinoma (PDAC) is characterized by accelerated production and degradation of hyaluronan (HA), a major component of extracellular matrix involved in the malignant phenotype of cancer. In particular, increased hyaluronidase (HYAL) activity plays a critical role in cancer progression, at least in part, by producing low-molecular-weight- (LMW-) HA or small fragments of HA, suggesting HYAL as a target for cancer treatment. Hyaluromycin, a new member of the rubromycin family of antibiotics, was isolated from the culture extract of a marine-derivedStreptomyces hyaluromycinias a HYAL inhibitor. We investigated the antitumor effects of hyaluromycin in PDAC cells. We examined the effects of hyaluromycin on the proliferation and migration of PDAC cells. To elucidate the mechanisms underlying the effect of hyaluromycin on PDAC cells, we examined the concentration of LMW-HA in the conditioned media after treating PDAC cells with hyaluromycin. We demonstrate that hyaluromycin inhibits proliferation and migration of PDAC cells. We also found that these antitumor effects of hyaluromycin were associated with a decreased concentration of LMW-HA and a decreased phosphorylation of ribosomal protein S6. Our results suggest that hyaluromycin is a promising new drug against this highly aggressive neoplasm.


2015 ◽  
Author(s):  
Andre R. Jordan ◽  
Juan Chipollini ◽  
Luis Lopez ◽  
Travis Yates ◽  
Vinata Lokeshwar

2015 ◽  
Vol 2 (1) ◽  
pp. 114 ◽  
Author(s):  
Enjuro Harunari ◽  
Chiaki Imada ◽  
Yasuhiro Igarashi ◽  
Takao Fukuda ◽  
Takeshi Terahara ◽  
...  

A novel hyaluronidase inhibitor (HI) was isolated from the culture extract of a marine- derived actinomycete strain. This strain MB-PO13 was isolated from ascidian (Molgula manhattensis) in Tokyo Bay. Out of about 1,000 isolates from various marine organisms, strain MB-PO13 had the strongest inhibitory activity and was selected for further study. The strain showed abundant-to-moderate growth on most media, forming a grayish mycelium. On the basis of the taxonomical characteristics, the strain was classified as belonging to the genus of Streptomyces and was named as Streptomyces sp. strain MB-PO13. The structure of HI was elucidated by interpretation of NMR data. HI displayed about 25-fold potent hyaluronidase inhibitory activity against hyaluronidase than glycyrrhizin. Keywords: marine actinomycetes; Streptomyces; hyaluronidase inhibitor 


2014 ◽  
Vol 24 (5) ◽  
pp. 498-504 ◽  
Author(s):  
Seon Gu Hwang ◽  
Anna Yang ◽  
Soo Jung Kim ◽  
Min Kee Kim ◽  
Sung Soo Kim ◽  
...  

Marine Drugs ◽  
2014 ◽  
Vol 12 (1) ◽  
pp. 491-507 ◽  
Author(s):  
Enjuro Harunari ◽  
Chiaki Imada ◽  
Yasuhiro Igarashi ◽  
Takao Fukuda ◽  
Takeshi Terahara ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document