treatment technologies
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2022 ◽  
Author(s):  
Natalia Klanovicz ◽  
Fábio Spitza Stefanski ◽  
Aline Frumi Camargo ◽  
William Michelon ◽  
Helen Treichel ◽  
...  

Abstract BACKGROUND Our study aimed to characterize and prospect immobilization strategies for a novel fungal peroxidase - POD (EC 1.11.1.7) and to insert it in the context of pollutant remediation, since these compounds pose risks to human and environmental health. The enzymatic extract was obtained by submerged fermentation of the fungus Trichoderma koningiopsis in an alternative substrate, consisting of fresh microalgal biomass. The immobilization efficiency was evaluated by monitoring the residual activity (RA) and the discoloration potential (DP) of a synthetic dye solution. Concomitantly, the catalytic properties of free POD were explored, and the most promising storage strategy to maintain the enzymatic activity was studied. RESULTS The novel guaiacol peroxidase expressed specific activity of up to 7801 U mg−1 in the free form, showing stability when subjected to up to 80°C in a pH range between 4.0-8.0. Furthermore, the bioproduct immobilized on magnetic nanoparticles expressed up to 689% RA and 100% DP. An increase in the RA of the enzyme, both in free and immobilized form, was also observed after storage for up to 8 months. The synthesized magnetic nanozymes showed good reusability, maintaining 13546 U mg−1 after ten cycles and removing 94% of color in a second batch. Toxicological evaluation with Allium cepa indicated that the enzymatic process of color removal with immobilized POD was essential to eliminate genotoxic effects. CONCLUSION T. koningiopsis peroxidase production and immobilization presented in our work are promising for the enzyme market and for the wastewater treatment technologies, considering its high bioxidative potential.


Author(s):  
Chinenye Adaobi Igwegbe ◽  
Ifeoma Amaoge Obiora-Okafo ◽  
Kingsley O. Iwuozor ◽  
Soumya Ghosh ◽  
Setyo Budi Kurniawan ◽  
...  

Water ◽  
2022 ◽  
Vol 14 (2) ◽  
pp. 140
Author(s):  
Motasem Alazaiza ◽  
Ahmed Albahnasawi ◽  
Gomaa Ali ◽  
Mohammed Bashir ◽  
Dia Nassani ◽  
...  

Pharmaceutical contamination threatens both humans and the environment, and several technologies have been adapted for the removal of pharmaceuticals. The coagulation-flocculation process demonstrates a feasible solution for pharmaceutical removal. However, the chemical coagulation process has its drawbacks, such as excessive and toxic sludge production and high production cost. To overcome these shortcomings, the feasibility of natural-based coagulants, due to their biodegradability, safety, and availability, has been investigated by several researchers. This review presented the recent advances of using natural coagulants for pharmaceutical compound removal from aqueous solutions. The main mechanisms of natural coagulants for pharmaceutical removal from water and wastewater are charge neutralization and polymer bridges. Natural coagulants extracted from plants are more commonly investigated than those extracted from animals due to their affordability. Natural coagulants are competitive in terms of their performance and environmental sustainability. Developing a reliable extraction method is required, and therefore further investigation is essential to obtain a complete insight regarding the performance and the effect of environmental factors during pharmaceutical removal by natural coagulants. Finally, the indirect application of natural coagulants is an essential step for implementing green water and wastewater treatment technologies.


2022 ◽  
pp. 118858
Author(s):  
Tianxiao Li ◽  
Hongxia Xu ◽  
Yuxuan Zhang ◽  
Hanshuo Zhang ◽  
Xin Hu ◽  
...  

Chemosphere ◽  
2022 ◽  
pp. 133553
Author(s):  
Vaibhav Shrivastava ◽  
Izba Ali ◽  
Makid Maskawat Marjub ◽  
Eldon R. Rene ◽  
Ailén María Florencia Soto

Author(s):  
Sobia Riaz ◽  
Zia Ur Rahman Farooqi ◽  
Predrag Ilic ◽  
Muhmmad Saad Bin Zafar ◽  
Hanan Ahmad ◽  
...  

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