ion mass spectrometry
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Author(s):  
Zebadiah Teichert ◽  
Maitrayee Bose ◽  
Peter Williams ◽  
Richard L. Hervig ◽  
Lynda B. Williams

2022 ◽  
Vol 572 ◽  
pp. 151467
Author(s):  
Sang Ju Lee ◽  
Aram Hong ◽  
Jinwan Cho ◽  
Chang Min Choi ◽  
Ji Young Baek ◽  
...  

2021 ◽  
Vol 9 (12) ◽  
pp. 2597
Author(s):  
Yukari Kuga ◽  
Ting-Di Wu ◽  
Naoya Sakamoto ◽  
Chie Katsuyama ◽  
Hisayoshi Yurimoto

Arbuscular mycorrhizal fungi are obligate symbionts of land plants; furthermore, some of the species harbor endobacteria. Although the molecular approach increased our knowledge of the diversity and origin of the endosymbiosis and its metabolic possibilities, experiments to address the functions of the fungal host have been limited. In this study, a C flow of the fungus to the bacteria was investigated. Onion seedlings colonized with Gigaspora margarita, possessing Candidatus Glomeribacter gigasporarum (CaGg, Gram-negative, resides in vacuole) and Candidatus Moeniiplasma glomeromycotorum (CaMg, Gram-positive, resides in the cytoplasm,) were labelled with 13CO2. The 13C localization within the mycorrhiza was analyzed using high-resolution secondary ion mass spectrometry (SIMS). Correlative TEM-SIMS analysis of the fungal cells revealed that the 13C/12C ratio of CaGg was the lowest among CaMg and mitochondria and was the highest in the cytoplasm. By contrast, the plant cells, mitochondria, plastids, and fungal cytoplasm, which are contributors to the host, showed significantly higher 13C enrichment than the host cytoplasm. The C allocation patterns implied that CaMg has a greater impact than CaGg on G. margarita, but both seemed to be less burdensome to the host fungus in terms of C cost.


2021 ◽  
Author(s):  
Pei Su ◽  
John P. McGee ◽  
Kenneth R. Durbin ◽  
Michael A. R. Hollas ◽  
Manxi Yang ◽  
...  

AbstractImaging of proteoforms in human tissues is hindered by low molecular specificity and limited proteome coverage. Here, we introduce proteoform imaging mass spectrometry (PiMS), which increases the size limit for proteoform detection and identification by 4-fold compared to reported methods, and reveals tissue localization of proteoforms at <80 μm spatial resolution. PiMS advances proteoform imaging by combining liquid sampling (nanospray desorption electrospray ionization, nano-DESI) with ion detection using individual ion mass spectrometry (I2MS). We demonstrate the first proteoform imaging of human kidney, identifying 169 of 400 proteoforms <70 kDa using top-down mass spectrometry and database lookup from the human proteoform atlas, including dozens of key enzymes in primary metabolism. Moreover, PiMS images visualize kidney anatomical structures and cellular neighborhoods in the vasculature versus the medulla or the cortex. The benefits of PiMS are poised to increase proteome coverage for label-free protein imaging of intact tissues.TeaserNano-DESI combined with individual ion mass spectrometry generates images of proteoforms up to 70 kDa.


Holzforschung ◽  
2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Dan Aoki ◽  
Yasuyuki Matsushita ◽  
Kazuhiko Fukushima

Abstract Various phenomena in living physiological systems are conducted on the hydrated conditions, and in many cases, they do not work in a dry state. Imaging mass spectrometry is one of the direct detection methods scanning the sample surface with some focused and pulsed energy and analysing the sputtered components. However, under the high vacuum conditions required for usual imaging mass spectrometry, the sample surface is rapidly dried. It is difficult for the target cell to survive, and the original situation are lost soon. Here, the combination of a freeze-fixation and a cryo sample stage is a promising method to do mass spectrometry while maintaining the original situation. By rapidly freezing the cells, the momentary situation as a living cell is fixed. The situation in a living cell can be captured as still images by cryo imaging mass spectrometry. This mini-review introduces the outline of imaging mass spectrometry especially for low molecular weight components and recent results for frozen-hydrated samples by cryo secondary ion mass spectrometry.


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