Dynamic Associations of Milk Components With the Infant Gut Microbiome and Fecal Metabolites in a Mother–Infant Model by Microbiome, NMR Metabolomic, and Time-Series Clustering Analyses

Background: The gut microbiome and fecal metabolites of breastfed infants changes during lactation, and are influenced by breast milk components. This study aimed to investigate dynamic associations of milk components with the infant gut microbiome and fecal metabolites throughout the lactation period in a mother–infant model.Methods: One month after delivery, breast milk and subsequent infant feces were collected in a pair for 5 months from a mother and an exclusively breastfed infant. Composition of the fecal microbiome was determined with 16S rRNA sequencing. Low-molecular-weight metabolites, including human milk oligosaccharides (HMOs), and antibacterial proteins were measured in feces and milk using 1H NMR metabolomics and enzyme-linked immunosorbent assays. The association of milk bioactive components with the infant gut microbiome and fecal metabolites was determined with Python clustering and correlation analyses.Results: The HMOs in milk did not fluctuate throughout the lactation period. However, they began to disappear in infant feces at the beginning of month 4. Notably, at this time-point, a bifidobacterium species switching (from B. breve to B. longum subsp. infantis) occurred, accompanied by fluctuations in several metabolites including acetate and butyrate in infant feces.Conclusions: Milk bioactive components, such as HMOs, might play different roles in the exclusively breastfed infants depending on the lactation period.

Processes of Nutrition, Metabolism, Biosynthesis of Milk Components and Vitality of Cows With High and Low-Fat Milk

The trials were performed on 20 multiparous cows of Holstein breed (39.7 ± 0.75 kg of milk) at the end of the first phase of lactation this different milk fat (4.1-2.8%). The aim of the research was to study the characteristics of nutrition, metabolism and biosynthesis of milk components in highly productive dairy cows with normal and low milk fat levels and the timing of their productive use. Study the characteristics of fermentation of scar formation substrates and their use in energy metabolism and biosynthesis of the milk components. Found that low fat milk is not associated with a lack of formation of acetate in the rumen (6.1 vs. 6.6 mmol/dl in the contents of the rumen, р>0.05) and the non change in the hormonal profile, but depends on the reduction of fatty acids synthesis de novo in mammary gland, regulated by conjugated higher fatty acids. The result is a reduction in the need of cows in the exchange energy (reduction of heat transfer by 6.2 MJ), a shorter service period (109.5 vs.139 days) and the prolongation of their productive use (the number of lactations correlated back with the level of fat in milk (r=-0.68, p<0.05, n=1300).

Studies and Application of Sialylated Milk Components on Regulating Neonatal Gut Microbiota and Health

Breast milk is rich in sialic acids (SA), which are commonly combined with milk oligosaccharides and glycoconjugates. As a functional nutrient component, SA-containing milk components have received increasing attention in recent years. Sialylated human milk oligosaccharides (HMOs) have been demonstrated to promote the growth and metabolism of beneficial gut microbiota in infants, bringing positive outcomes to intestinal health and immune function. They also exhibit antiviral and bacteriostatic activities in the intestinal mucosa of new-borns, thereby inhibiting the adhesion of pathogens to host cells. These properties play a pivotal role in regulating the intestinal microbial ecosystem and preventing the occurrence of neonatal inflammatory diseases. In addition, some recent studies also support the promoting effects of sialylated HMOs on neonatal bone and brain development. In addition to HMOs, sialylated glycoproteins and glycolipids are abundant in milk, and are also critical to neonatal health. This article reviews the current research progress in the regulation of sialylated milk oligosaccharides and glycoconjugates on neonatal gut microbiota and health.

PSVIII-14 Effect of Certillus, a direct-fed microbial, on milk yield, milk components, and feed intake in primi- and multiparous lactating Holstein cows

To determine the effect of Certillus on milk production, milk components and feed efficiency, primiparous (n = 13) and multiparous (n = 12) Holstein cows were assigned to one of two dietary groups starting from 8 to 120 days of lactation. The controls (n = 13) received regular TMR ration, and treated cows (n =12) received control TMR plus 2 x 109 cfu/head of Certillus (Church & Dwight Co., Inc., Waukesha, WI). Cows were housed in an open-air free-stall barn with 16 Insentec electronic feeders (8 feeders/group) to record daily feed events and intake (DMI). Cows were provided with ad libitum TMR fed twice daily and were milked twice daily. Milk samples were taken weekly during successive a.m./p.m. milkings and analyzed for percentage milk fat, protein, lactose, and milk urea nitrogen (MUN). Data were analyzed using a repeated-measures 2 x 2 factorial ANOVA. Daily milk production (4%FCM) was influenced (P < 0.05) by treatment x parity, week and parity, averaging 11% greater (P < 0.05) in Certillus-fed than control cows. Milk fat percentage increased (P < 0.005) from 4.02 to 4.41 + 0.09% with Certillus feeding. Milk lactose was influenced (P < 0.05) by week and treatment x parity. Milk protein was affected by week (P < 0.001) only. Certillus decreased (P < 0.05) MUN levels such that MUN was 1.0 mg/dL greater (P < 0.001) in Certillus-fed than control cows. Feed intake was reduced by 9% (P < 0.01) and feed efficiency (kg 4%FCM/kg DMI) was improved by 14% (P < 0.05) in Certillus-fed versus control cows. We conclude that Certillus may hold potential as an effective direct-fed microbial to increase feed efficiency and milk production.

PSIV-11 Effects of feeding a 50% cranberry vine pellet on milk components during lactation in sheep

The objective of this study was to examine the effect of feeding cranberry vine (CV) on milk components during early lactation as part of a larger study on the antiparasitic efficacy of CV supplementation on ewes during the periparturient period. Ewes were fed a 50% CV pellet (CVP; n = 12) or a control pellet (CON; n = 13) beginning at 102±1 d of pregnancy until d 65±1 postpartum. The CV pellet fed was formulated to be equivalent in digestible dry matter to the control pellet. BW was determined weekly during the study and milk samples were collected weekly during lactation. Data were analyzed in SAS with repeated measures. Ewe BW were similar at the start of study (180.36lbs ± 4.70lbs; P = 0.43) however by wk 8 postpartum CVP ewes weighed less than CON ewes (CON: 212.31lbs±7.86lbs; CVP: 185.58lbs±8.00lbs; P = 0.04). There was a treatment*wk effect observed for milk fat, protein and MUN (P ≤ 0.05). Ewes fed CVP exhibited reduced milk fat at wks 2 (CON: 6.75%±0.63%; CVP: 6.06%±0.58%; P = 0.03) and 5 (CON: 6.66%±0.37%; CVP: 5.54%±0.26%; P = 0.05), milk protein was reduced in CVP ewes at wk 2 (CON: 4.92%±0.12%; CVP: 4.72%±0.19%; P = 0.05) and MUN was greater at weeks 4 (CON: 22.89±0.7mg/dL; CVP: 27.93±0.85mg/dL; P = 0.01) and 8 (CON: 24.80±0.51mg/dL; CVP: 26.64±0.86mg/dL; P < 0.01) in CVP ewes. Somatic cell analysis is pending. In conclusion, CVP supplementation during lactation affects milk composition in sheep. Studies are underway to determine the effect of CV supplementation on additional metabolic parameters in pregnant and lactating ewes.

63 Effect of Chicory Silage and Se-yeast on Peripartum Ewes: Performance Data

The objective of this study was to test the possibility of improving the performance of peripartum ewes by feeding a combination of chicory silage and Se-yeast. Approximately four weeks prior to lambing, 36 pregnant Polypay ewes were moved into single pens and randomly assigned to one of four groups to be fed either grass or chicory silage and supplemented or not with 3.6 mg Se/day (DiaMune, Diamond V). Daily dry matter intake (DMI), water intake, body weight gain of lambs and body weight changes of the ewes, milk yield using a weigh-suckle-weigh system, and milk components were recorded. Data were analyzed using GLIMMIX (SAS v9) with time, silage, and Se interaction as fixed effects and ewe as random effect. Significance was declared with P ≤ 0.05. The DMI was lower in ewes fed chicory compared to grass silage with no effect of Se. Water intake was greater in ewes that were fed chicory silage than grass silage but lower in those supplemented with Se. Milk production of ewes and BW gain of the lambs were not affected by the treatments, but the gain efficiency (G:F) of lambs was larger in animal fed chicory vs. grass silage. Few differences were observed in milk components between the treatments. Protein content and SNF tended to be reduced by feeding Se (P = 0.08) while feeding Se increased C18:0 and preformed fatty acids in milk. Feeding chicory+Se tended to increase percentage of de novo-derived fatty acids in milk compared to feeding grass+Se. Somatic cell count (SCC) was not affected by the treatments. Overall, our data indicated that performance of the animals as well as milk components were not affected by feeding chicory and/or Se despite a lower feed intake when chicory was fed. Higher feed efficiency by using chicory is suggested by the DMI/gain of lambs.

64 Feeding Chicory-plantain Silage and Se-yeast on Lactating Ewe Subjected to Intramammary Infection: Effect on Performance and Milk Quality

Our objective was to test the effect on milk yield and quality of feeding a combination of chicory-plantain and Se-yeast in lactating ewes subjected to intramammary infection (IMI). For this purpose, we enrolled 28 Polypay ewes from a prior study where they received chicory (CS) or grass (GC) silage and supplemented with 3.6 mg of Se/day as Se-yeast (DiaMune, Diamond V) (Y) or not (C) for 2 months. For the present study, ewes were kept on the original dietary regiment except the CS group received a chicory-plantain silage (50% each). Ewes were milked twice daily and milk was collected for analysis of components and somatic cell count (SCC). IMI was induced by injecting 2×107 CFU of Strep. uberis in both glands. Data were analyzed using GLIMMIX (SAS) with time, silage, and Se and their interactions as the fixed effects and ewe as random effect with significance declared P ≤ 0.05. SCC increased 8-fold 24h post-IMI and remained at that level until the end of the experiment with higher values pre-IMI in GC-Y animals but no difference post-IMI. Milk yield drastically decreased post-IMI but was not affected by treatments. Protein, fat, solids, amount of de novo fatty acids, and amount of C16:0 were larger in milk of ewes fed chicory-plantain compared to grass silage. Supplementation of Se had minor effect on milk components with a tendency (P = 0.07) to decrease fat, solids, and amount of de novo fatty acids. Amounts of C18:0, C18:1, and preformed fatty acids in milk were increased by Se supplementation in ewes fed grass while decreased in ewes fed chicory. The use of Strep. uberis was effective in inducing IMI in ewes with chicory-plantain improving several milk components while Se supplementation had minor effects. The combination of chicory and Se did not improve the response to IMI.