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2022 ◽  
Vol 52 (4) ◽  
Author(s):  
Bruno Tabarelli Scheidt ◽  
Evandro Zacca Ferreira ◽  
Flávio Chupel Martins ◽  
Juliano Berghetti ◽  
Marília Michalski De Pieri ◽  
...  

ABSTRACT: The fungus Microdochium albescens can interfere in the germination of seeds and in the death of rice seedlings; however, there is not technical indication for its control by seed treatment. This research evaluated the efficiency of fungicides in the treatment of pre-germinated and dryland rice seeds in the control of M. albescens. Seeds of the cultivars, Epagri 109, SCS116 Satoru, SCS121 CL and SCS122 Miura were treated with the following fungicides (g a.i./100 kg of seeds): fluazinam + thiophanate methyl (9.45 + 63), pyraclostrobin + thiophanate methyl (5 + 45), carboxin + thiram (60 + 60), metalaxyl-M + thiabendazole + fludioxonil (3 + 22.5 + 3.75), carbendazim + thiram (45 + 105), carbendazim (45). Seeds without fungicide treatment constituted the control. The treated seeds were sown in potato-sucrose-agar (PSA) culture medium and incubated at 25 ± 2 °C and photoperiod of 12 hours for 14 days. Treated seeds were also submitted to the germination test. Fluazinam + thiophanate methyl and metalaxyl-M + thiabendazole + fludioxonil showed greater control of M. albescens, not differing statistically between them, in the five cultivars and in the two cultivation systems. The tested fungicides provided seed germination above 90% in the four cultivars.


2021 ◽  
pp. 410-420
Author(s):  
Xiaoshuang Zhang ◽  
Dequan Zhu ◽  
Kang Xue ◽  
Lanlan Li ◽  
Jianjun Zhu ◽  
...  

To improve the adaptability and precision of the slider-hole-wheel seed-metering device to meet the requirements of precision sowing, the single factor simulation experiments and the three factors three levels of orthogonal simulation experiments were carried out based on the discrete element method. The rotation speed of the seeding shaft, the shape of the hole, and the depth of the hole were set as experiment factors. The results of simulation experiments showed that the qualified rate was the highest when the rotation speed of the seeding shaft was 30 r/min, the shape of the hole was oval, and the depth of the hole was 9 mm. The qualified rate, replay rate, and miss-seeding rate were 89.09 %, 3.64 %, and 7.27 %,respectively. The hybrid rice seeds of Zhongnong 2008, Chuangliangyou 4418, and Gangyou 898 were chosen as the materials for the bench and field seeding performance tests to verify the reliability of the simulation results. The test results showed that the qualified rate of Zhongnong 2008, Chuangliangyou 4418, and Gangyou 898 seed in bench tests were 85.07 %, 85.20 %, and 82.13 %, and the qualified rate of Zhongnong 2008, Chuangliangyou 4418, and Gangyou 898 seed in field tests were 82.13 %, 82.27 %, and 80.53 %. The seeding performance with the three kinds of rice seeds could meet the agronomic requirements for precision sowing of hybrid rice. The paper provided the basis for the structure optimization and seeding performance improvement of the slider-hole-wheel seed-metering device.


2021 ◽  
Vol 20 (4) ◽  
pp. 278-285
Author(s):  
Jaquelini Garcia ◽  
Camile Thais Castoldi ◽  
Gisiane Camargo de Andrade ◽  
Cileide Maria Medeiros Coelho ◽  
Virgílio Gavicho Uarrota

O arroz é uma das espécies que apresenta dormência após a colheita, podendo esta ser prolongada durante o armazenamento das sementes. Este trabalho teve por objetivo determinar se o ácido giberélico (GA3) é um promotor eficiente da superação de dormência em sementes de arroz e avaliar mudanças nas estruturas biológicas via histoquímica. A cultivar utilizada foi a SCS122 Miura submetida a 0 mg L-1, 500 mg L-1 e 1000 mg L-1 de GA3. Foram realizadas análises de germinação, viabilidade, comprimento de raiz, parte aérea e plântula, microscopia óptica do amido e quantificação dos açúcares solúveis totais. A utilização de 500 mg L-1 e 1000 mg L-1 de GA3 foi eficiente para a superação da dormência de sementes de arroz, reduzindo o percentual de sementes dormentes para 4% e 1% respectivamente. Apesar de reduzir o percentual dormência, a presença de GA3 provoca aumento do percentual de plântulas anormais, e por isso, nas concentrações utilizadas, não pode ser recomendado como método de superação em sementes de arroz. A microscopia óptica é eficiente para verificar que com a superação de dormência, ocorre a degradação dos grânulos de amido, aumentando a disponibilidade de açúcares solúveis totais para o crescimento e desenvolvimento de plântulas.


2021 ◽  
Vol 34 (4) ◽  
pp. 791-798
Author(s):  
RAFAEL MARANI BARBOSA ◽  
MATHEUS ANDRÉ DE JESUS ◽  
RAFAELA ALVES PEREIRA ◽  
GEDEON ALMEIDA GOMES JUNIOR

ABSTRACT To evaluate seed vigor, electrical conductivity and ethanol tests are fast and efficient methodologies. They have the potential to be used in several species, such as red rice. However, there are no protocols or information about their efficiency. Thus, the objective was to evaluate the efficiency, and define parameters of execution for electrical conductivity and ethanol tests, to evaluate the vigor of red rice seeds. The study was conducted using four lots of ‘BRS 901’ red rice, which was subjected to a germination test, as well as first count, accelerated aging, and field seedling emergence tests. The electrical conductivity test was performed with 25, 50, and 100 seeds soaked in 50 mL and 75 mL of water, at 25 °C and 30 °C, for 3, 6, 20, and 24 hours, respectively. The ethanol test was performed with 50 and 100 seeds soaked in a volume of water equivalent to 1.0, 1.5, 2.0, 2.5, and 3.0× the mass of the seed sample. To assess the vigor of red rice seeds, the electrical conductivity test is an efficient method when conducted with 50 seeds soaked in 50 mL of water at 25 °C for 20 hours. Meanwhile, the ethanol test is most effective when performed with 50 seeds, in a volume of water that is 2.5× the mass of the sample, at 40 °C for 24 hours.


Cells ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 3361
Author(s):  
Waka Ishida ◽  
Tatsuma Kishimoto ◽  
Fumio Takaiwa ◽  
Ken Fukuda

We investigated the prophylactic and therapeutic effects of the oral administration of transgenic rice seeds expressing a hypoallergenic Bet v 1 derivative of allergic birch pollen conjunctivitis in mice. Transgenic rice seed depositing a chimeric molecule called TPC7 (tree pollen chimera 7) created by DNA shuffling of Bet v 1 family sequences from birch, alder and hazel in protein bodies of endosperm was generated. BALB/c mice were sensitized to birch pollen in alum and challenged with pollen in eyedrops. They were fed TPC7 transgenic or non-transgenic (control) rice seeds for 14 d before sensitization (prophylactic protocol) or 17 d after sensitization (therapeutic protocol). The clinical score and number of conjunctival eosinophils were significantly lower in TPC7-fed mice than in the control mice based on both the prophylactic and therapeutic protocols. Serum concentration of allergen-specific IgE did not differ between TPC7-fed and control groups in either protocol. Prophylactic administration of TPC7 downregulated the production of IL-4 and IFN-γ, whereas therapeutic administration of TPC7 upregulated the production of IFN-γ by allergen-stimulated splenocytes. Prophylactic or therapeutic oral administration of transgenic rice expressing TPC7 suppressed birch pollen-induced allergic conjunctivitis in mice. Feeding transgenic rice is a potentially effective approach as an allergen-specific immunotherapy for allergic conjunctivitis.


2021 ◽  
Author(s):  
Musa Ibrahim Saheed ◽  
Beckley Ibrahim Ikhajiagbe

The research investigated the possibility of phosphate solubilizing bacteria (PSB) with plant growthpromoting (PGP) capabilities to improve growth properties of rice plant under ferruginous ultisol (FU) condition through bio-priming strategy. The PSB with PGP properties used in this research were Bacillus cereus strain GGBSU-1, Proteus mirabilis strain TL14-1 and Klebsiella variicola strain AUH-KAM-9 that were previously isolated and characterized following the 16S rRNA gene sequencing. Biosafety analysis of the PSB isolates was conducted using blood agar. The rice seeds were then bio-primed with the PSBs at 3, 12 and 24 hours priming durations and then sown in a composite FU soil sample. Differences in germination bioassay involving SEM, morphology, physiology and biomass parameters were investigated for 15 weeks after bio-priming. The composite FU soil used in the study had high pH, low bioavailable phosphorus, low water holding capacity and high iron levels which has led to a low growth properties of rice seeds without bio-priming in FU soil. Germination parameters was better in seeds bio-primed with the PSBs, especially at 12h priming duration as against seeds without priming. SEM showed more bacterial colonization in the PSB bioprimed seeds. Seed bio-priming of rice seed with Bacillus cereus strain GGBSU-1, Proteus mirabilis strain TL14-1 and Klebsiella variicola strain AUH-KAM-9 under FU soil condition significantly improved seed microbiome, rhizocolonization and soil nutrient properties, thereby enhancing growth properties of the rice plant. This suggest the ability of PSB to solubilize and mineralize soil phosphate and improve its availability and soil property for optimum plant usage in phosphate stressed and iron toxic soils.


2021 ◽  
Author(s):  
Musa Saheed Ibrahim ◽  
Beckley Ikhajiagbe

Abstract Background The research investigated the possibility of phosphate solubilizing bacteria (PSB) with plant growth-promoting (PGP) capabilities to improve growth properties of rice plant under ferruginous ultisol (FU) condition through rhizo-inoculation strategy. The PSB with PGP properties used in this research were Bacillus cereus strain GGBSU-1, Proteus mirabilis strain TL14-1 and Klebsiella variicola strain AUH-KAM-9 that were previously isolated and characterized following the 16S rRNA gene sequencing. The rice seeds were sown in a composite FU soil sample and a humus soil (control) and then rhizo-inoculated along the root region of the growing rice seedling at 16 days after sowing. The rice plant was studied for differences in morphological, physiological and biomass parameters for 16 weeks after rhizo-inoculation. Results The composite FU soil used in the study had high pH, low bioavailable phosphorus, low water holding capacity and high iron levels which has led to a low growth properties of rice seeds sown in FU soil without rhizo-inoculation. After rhizo-inoculation, a significant increase in morphological, biomass and physiological parameters were observed in the rice plant grown in the FU soil as against the control and the rice plant in FU soil without inoculation except for terpenoid which is usually known to signify biotic stress and as part of plant defense mechanism. Generally, Bacillus cereus strain GGBSU-1 showed higher improvement compared to other PSBs used in the present study. This is due to a more improved growth properties observed. Conclusion Rhizo-inoculation of rice seedling with Bacillus cereus strain GGBSU-1, Proteus mirabilis strain TL14-1 and Klebsiella variicola strain AUH-KAM-9 under FU soil condition significantly improved growth properties of the rice plant. This suggest the ability of PSB to solubilize and mineralize soil phosphate and improve its availability for plant use in phosphate stressed soil, thereby improving plant growth properties.


2021 ◽  
Author(s):  
Zhishan Wang ◽  
Yongqiang Zhu ◽  
Ni Li ◽  
Biqi Sun ◽  
Hai Liu ◽  
...  

Abstract Upland rice has the characteristics of strong drought tolerance and wide adaptability. Cultivating upland rice with high yield and high quality can solve the contradiction between food shortage, water shortage, and population increase in countries all over the world, and is of great significance to the sustainable development of agriculture. In this study, high-throughput sequencing technology based on the Illumina Miseq platform was used to investigate the structure and diversity of endophytic bacterial communities using 12 upland rice variety seeds from different areas in Yunnan Province of China as materials. This study aims to reveal the "core microbiota" of the endophytic bacteria in upland rice seeds in the Yunnan Province of China by examining their diversity and community structures. The results showed that 39 endophytic OTUs were found to coexist in all samples. At the phylum level, the first dominant phyla in the 12 seed samples were Proteobacteria (66.92-99.98%). At the genus level, Pantoea (9.75-99.24%), Pseudomonas (0.11-37.24%), Curtobacterium (0.01-19.90%), Microbacterium (0.01-14.95%), Methylobacterium (0.40-5.86%), Agrobacterium (0.01-4.53%), Sphingomonas (0.04-1.56%), Aurantimonas (0.01-1.45%) and Rhodococcus (0.11-1.09%) served as the dominant genera that coexisted in all the upland rice seeds tested and represent the core microbiota in upland rice seeds. Through the correlation analysis with upland rice habitat environmental factors, the effects of climate and altitude on the structure and diversity of endophytic bacterial community in upland rice seeds were further revealed. The results showed that environmental factors such as temperature, precipitation and altitude have great influences on the structure of endophytic bacterial community in upland rice seeds. This study is of great significance to explore the relationship between upland rice and its endophytic bacteria and to tap the resources of drought-tolerant bacteria to improve the yield of local upland rice.


2021 ◽  
Vol 883 (1) ◽  
pp. 012014
Author(s):  
B Kurniasih ◽  
N Arini ◽  
D Alvioliana ◽  
R I Nisa ◽  
R A Wulandari

Abstract Climate change will increase the occurrence of salinity in agricultural land along with the coastal areas. One of the technologies to reduce salinity is NaCl pretreatment. This study aimed to evaluate salinity treatment's effect during nurseries on the growth of lowland rice seedlings. There were three separate experiments, and all the experiments used Randomized Complete Block Design. In the first experiment, local black rice seeds (var. Jelitheng) was used. The nursery was carried out at three salinity levels, i.e. 0.2, 3 and 5 dS/m. The second experiment was conducted using salt-resistant rice seeds (var. Dendang) and salt susceptible rice seeds (var. IR 64). The salinity levels applied were non-saline (0.2 dS/m) and saline (5 dS/m). The third experiment used rice seedling var. IR 64, with the first factor being the salinity level (0.2 and 5 dS/m) and the second factor was a wet nursery and dry nursery. In general, the results from the three experiments showed that giving salinity levels of 3-5 dS/m in several rice varieties improved seedling performance. Although salinity during nursery could increase the concentration of Na+ and decrease the concentration of K+ in leaves, salinity during nursery increased the seedlings fresh weight, and dry weight increased the number of seedlings leaves and increased the concentration of leaf chlorophyll. The better seedlings growth variable in the saline nursery will help the plants cope with salinity in the later growth stage in the field.


2021 ◽  
Vol 17 (2) ◽  
pp. 258-273
Author(s):  
Atang Muhammad Safei ◽  
Siti Amanah ◽  
Anna Fatchiya

The availability of certified rice seeds is still limited because the limited number of rice seed breeder farmers.The capacity of farmers in seed assessment and seed production determine the succes of the rice seed breeding. The research using the census method (112 breeder farmers) was conducted in Majalengka Regency. Interviews and observations were carried out to obtain data on extension and breeding of rice seeds by farmers. Partial Least Square (PLS-Path Modeling) was used to analyze the data. The results showed that the suitability of the materials, extension methods and ability of the extension agents were in the medium category, the intensity of extension was still low. Extension officers visit farmer groups once a month. The role of farmer groups and farmer capacity is in the medium category. Farmer groups are able to carry out the learning process and seed production even though the marketing is not continuous. The implementation of extension services and farmer groups has a positive effect on the farmer capacity. The use of social media by breeder farmers for seed marketing and obtaining marketing partners and business capital can be increased through increasing the intensity of extension by involving farmer groups.


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