Degradation kinetics and in vitro digestive stability of selected bioactive compounds from a beverage formulated with butterfly pea flowers

ABSTRACT: This study evaluated the effects of temperature on the pH of extracts of ascorbic acid and anthocyanins from petals of butterfly pea, as well as their in vitro digestive stability in model systems at 60, 70, and 80 °C. The pH values significantly decreased with an increase in the temperature (P < 0.05). The findings were similar for the degradation of anthocyanins and ascorbic acid, which followed first-order kinetics in all the systems. The samples heated at 80 °C presented the highest degradation rate (kobs), as well as higher percentages of degradation at the end of digestive stability in vitro.

The Potential of Clitoria ternatea L. Extracts as an Alternative Indicator in Acid-Base Titration

Experiments are an important part of the chemistry curriculum. An indicator in the form of a synthesis indicator is commonly used in acid-base materials experiments. The usage of synthetic indicators can result in waste that is both environmentally harmful and costly. The butterfly pea flower is one of the plants that contains anthocyanins, which have the potential to be used as an alternative indication of natural acids and bases. The study aims to determine the acid and base material curriculum and competency indicators, as well as the potential of butterfly pea flower extract as a substitute for synthesis indicators and the practicality of butterfly pea flower extract as a chemical experimental design in SMA/MA. This study employs a descriptive qualitative research method that includes literature review, observation, experimentation, and interviewing. The results showed that the relationship between acid-base materials and experiments was the determination of natural materials as acid-base indicators, the pH of the solution, the identification of acid-base properties, and acid-base titration. The butterfly pea flower indicator was produced from maceration extraction using 96% ethanol as the solvent. The butterfly pea flower indicator gives pink color at pH 1-2, reddish purple at pH 3, light purple at pH 4-5, turquoise at pH 6, bluish green at pH 7, light blue at pH 8-9, green at pH 10, yellowish green at pH 11, greenish yellow at pH 12-13, and yellow at pH 14. The titration step showed that butterfly pea flower indicators could replace synthetic indicators, namely phenolphthalein and methyl orange. Based on the analysis, the butterfly pea flower indicator can be used as an alternative indicator in acid base titration and an alternative experimental design in schools.

Impact of different thin layer drying temperatures on the drying time and quality of butterfly pea flowers

With attractive flower colours ranging from dark green to purple, Butterfly pea (Clitoria ternatea L.) is grown year-round in Vietnam. The purpose of this study is to determine the effect of air temperature on drying time and antioxidant compounds of Butterfly pea flowers, fitting the drying curves and testing the goodness of fit. In this study, air drying characteristics of the Butterfly pea flowers were determined using drying air temperature from 55oC to 70oC at a constant air velocity of 1 m/s. The data of experimental moisture loss were fitted to selected seven thin-layer drying models. The effect of drying conditions on the anthocyanin and total phenolic compound changes of Butterfly pea flower were compared. The effect of temperature on the diffusivity was described using the Arrhenius equation with an activation energy of 71.63 kJ.mol- ¹. At increasing temperature, the effective moisture diffusivity values ranged from 2.39×10-12 and 7.76×10-12 m²s - ¹. The mathematical models were compared according to the three statistical parameters such as the coefficient of determination (R2 ), reduced chi-square (χ 2 ) and root mean square error (RMSE) between the observed and predicted moisture ratios. The highest value of R2 (99.8%) and the lowest values of χ 2 (0.0004) and RMSE (0.0178) were observed for drying air temperature of 70oC. Among the seven mathematical models tested with experimental data, the Page model could sufficiently be described the drying characteristics of the Butterfly pea flower.

Spectral Characteristic, Storage Stability and Antioxidant Properties of Anthocyanin Extracts from Flowers of Butterfly Pea (Clitoria Ternatea L.)

Anthocyanins from flowers of the butterfly pea (Clitoria ternatea L.) are promising edible blue food colorants. Food processing often faces extreme pHs and temperatures, which greatly affects the color and nutritional values of anthocyanins. This study explored the color, spectra, storage stability, and antioxidant properties of C. ternatea anthocyanin extract (CTAE) at different pHs. The color and absorption spectra of CTAEs at a pH of 0.5–13 were shown, with their underlying structures analyzed. Then, the storage stability of CTAEs were explored under a combination of pHs and temperatures. The stability of CTAE declines with the increase in temperature, and it can be stored stably for months at 4 °C. CTAEs also bear much resistance to acidic and alkaline conditions but exhibit higher thermal stability at pH 7 (blue) than at pH 0.5 (magenta) or pH 10 (blue-green), which is a great advantage in food making. Antioxidant abilities for flower extracts from the butterfly pea were high at pH 4–7, as assessed by DPPH free radical scavenging assays, and decreased sharply when the pH value exceeded 7. The above results provide a theoretical basis for the application of butterfly pea flowers and imply their great prospect in the food industry.

Microencapsulation of three natural dyes from butterfly pea, Sappan wood, and turmeric extracts and their mixture base oncyan, magenta, yellow (CMY) color concept

Synthetic dyes in food can cause severe problems for health, so they need to be replaced by natural dyes. However, natural dyes are unstable, and encapsulation is one way to maintain the stability of natural dyes. This study was conducted to determine the best microencapsulation coating, storage stability, and color variations produced by butterfly pea, sappan wood, and turmeric extracts. The coating materials used were maltodextrin, carrageenan, and carboxy methyl cellulose (CMC) using the following formulations: 85% maltodextrin and 15% carrageenan (formula A) and 90% maltodextrin and 10% carrageenan (formula B) for coating butterfly pea and sappan wood extracts. Turmeric extracts were coating using 85% maltodextrin and 15% carrageenan (formula A) and 75% CMC and 25% starch (formula C). The encapsulation with maltodextrin (90%) and carrageenan (10%) was the best of encapsulation formula for butterfly pea and sappan wood extract. However, the encapsulation with maltodextrin (85%) and carrageenan (15%) was the best of encapsulation formula for turmeric extract. The green color was obtained from mixing butterfly pea and turmeric dyes in 1:4 ratio, purple from mixing butterfly pea and sappandyes in 1:8 ratio, and orange from mixing turmeric and sappan dyes in 1:2 ratio.

Using a Participatory Learning and Action Approach to Improve Young Papuans and Moluccans’ Knowledge of Herbal Drinks to Increase Immunity During the COVID-19 Pandemic

The introduction of coronavirus disease (COVID-19) into the human population represents a tremendous medical and economical crisis. The immune system plays a central role in protecting against this novel virus. This activity aimed to empower young generation using participatory learning and action approach to improve their knowledge about medicinal plants that grow in Indonesia and whose anti-inflammatory, antioxidant, immunomodulatory, and antiviral properties. Organoleptic test results showed that Kunyit Asam (consisted of turmeric and ginger) and Tealang (consisted of butterfly pea and lemongrass) were highly favored by panelists. Our results provide valuable information on the medicinal plants used as an immunity booster within the studied community.