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Agronomy ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 16
Author(s):  
Elena Myagkikh ◽  
Svetlana Babanina ◽  
Alexander Mishnev ◽  
Ludmila Radchenko ◽  
Vladimir Pashtetskiy ◽  
...  

Since the registry of common oregano (Origanum vulgare L.) cultivars does not involve regionalization, a comprehensive study of cultivars bred by different institutions in the intended cultivation region is valuable and relevant. The objective of the research was to assess the possibility of using various indices of ecological adaptability originally developed for grain crops for their use in the most adapted genotypes’ selection (breeding samples and cultivars) of Origanum vulgare L. to the temperate climate of the Crimean Peninsula. The research was carried out in the piedmont zone of Crimea from 2016 to 2019. The study material consisted of breeding samples No. 10 and No. 82 from the collection of FSBSI “Research Institute of Agriculture of Crimea”, as well as cultivars Zima, Raduga, and Slavnitsa selected by the All-Russian Scientific Research Institute of Medicinal and Aromatic Plants (ARSRIMAP). Genotype had the greatest influence on yield of fresh oregano material (43%) with the influence of the weather conditions of the year being 2%. On the contrary, meteorological conditions had a much greater effect on the essential oil accumulation and its areal yield, which were 30% and 25%, respectively. In terms of the coefficient of ecological variation of fresh yield, sample No. 82 and Slavnitsa cultivar were the best (11.47–16.7%). The local genotypes No. 10 and No. 82 varied less by the essential oil content and its yield. The genotype effect value was greater than 0 in the Raduga cultivar and local genotype No. 82 for the yield, but only in No. 82 genotype for the other two characteristics. Cultivars Zima and Raduga were classified as intensive (bi > 1) by the environmental flexibility of fresh yield, while local genotype No. 82 and Slavnitsa cultivar formed the group of intensive ones by essential oil content and essential oil yield. Local genotypes No. 10 and No. 82 were better than the introduced cultivars in terms of essential oil content homeostability and essential oil yield (Hom = 1.91–2.18). Thus, local genotypes proved to be more adapted to the region’s conditions in terms of essential oil accumulation. However, they were inferior to the registered cultivars of ARSRIMAP breeding in terms of fresh yield.


2021 ◽  
Author(s):  
А. Дягилева ◽  
◽  
Лидия Туманова ◽  
Валентин Митин ◽  
Кристина Грэждиеру ◽  
...  

In this paper the results of molecular diagnostics of Fusarium spp. and Alternaria spp. in bell pep-per and eggplant seeds of local genotypes at different time points of storage are presented. The diagnos-tics was effectuated using nested-PCR protocol with genus-specific and species-specific primers to F. ox-ysporum, F. solani, F. nivale, F. equiseti, F. culmorum, F. verticillioides, F. avenaceum, A. alternata, and A. solani. In the samples of studied bell pepper and eggplant genotypes A. alternata was found. In egg-plant seeds certain species of Fusarium spp. were identified.


Author(s):  
Şemsettin Kulaç ◽  
Hatice Nihan Nayır

In this study, to reproduce natural chestnut genotypes, the fruit characteristics of the Düzce region determined by local people are good in a healthy way, compatible with Marigoule (C. sativa × C. crenata) hybrid varieties resistant to branch cancer and root rot was investigated. In this study, 24 different chestnut genotypes were used. As the grafting method, the most commonly used tongue grafting, split grafting, chip budding grafting, and side grafting method were used. All procedures related to grafting were carried out in the greenhouses of the Düzce University Faculty of Forestry. In this study, local genotypes that are compatible with Marigoule chestnut were determined. As a result, the native genotype, which showed the best adaptation to Marigoule seedlings, was 87.5% of Yalnızçam, and after that, 79.2% of the Ereğli Sefer genotype. The lowest fit showed Broken genotype with 15% and Akçakoca1genotype with 17.5%. Besides, the compatibility of foreign varieties Maraval, Marigoule, and Betizac were also investigated. As a result, Betizac had the highest compatibility with 95%, while Maraval had 67.5%. The most successful in the grafting methods applied was the side graft (74.2%) followed by tongue grafting (59.9%), splitting grafting (51.4%), and chip budding grafting (29.7%).


Foods ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1371
Author(s):  
Wilma Sabetta ◽  
Isabella Mascio ◽  
Giacomo Squeo ◽  
Susanna Gadaleta ◽  
Federica Flamminii ◽  
...  

This research focuses on the exploration, recovery and valorization of some minor Italian olive cultivars, about which little information is currently available. Autochthonous and unexplored germplasm has the potential to face unforeseen changes and thus to improve the sustainability of the whole olive system. A pattern of nine minor genotypes cultivated in three Italian regions has been molecularly fingerprinted with 12 nuclear microsatellites (SSRs), that were able to unequivocally identify all genotypes. Moreover, some of the principal phenolic compounds were determined and quantified in monovarietal oils and the expression levels of related genes were also investigated at different fruit developmental stages. Genotypes differed to the greatest extent in the content of oleacein (3,4-DHPEA-EDA) and total phenols. Thereby, minor local genotypes, characterized by stable production and resilience in a low-input agro-system, can provide a remarkable contribution to the improvement of the Italian olive production chain and can become very profitable from a socio-economic point of view.


Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3600
Author(s):  
Fhatuwani Thovhogi ◽  
Godwin Richard Ainamensa Mchau ◽  
Eastonce Tendayi Gwata ◽  
Nombasa Ntushelo

Spider plant (Cleome gynandra L.) is an important leafy vegetable that grows naturally in many parts of the world. The leaves are highly nutritious and are used mainly for human consumption. The mineral content and phenolic compounds of 17 genotypes (local and exotic) of spider plant and four standards (swiss chard, jute mallow, cowpea, and pumpkin) were investigated. Leaf samples were harvested from plants raised at Thohoyandou, South Africa. Exotic genotypes were superior to local genotypes for most of the minerals. Swiss chard possessed significantly high levels of some minerals such as iron and manganese in comparison with exotic spider plant genotypes. The calcium content in the local (‘MP-B-3-CG’) and exotic (‘GPS’) genotypes was >30.0% and >60.0% higher than in swiss chard, respectively. Total phenolics among spider plant genotypes ranged from 9.86 to 12.21 mg GAE/g DW and were superior to pumpkin. In addition, the spider plant genotypes varied significantly in the antioxidant capacity as estimated by the 2,2 diphenyl-1-picrylhydrazyl method and ferric-reducing antioxidant power. The main flavonoid in the leaves of spider plant genotypes was quercetin-3-rutinoside. Crotonoside (glycoside) was detected in all the spider plant genotypes and swiss chard. A positive correlation was observed between total phenolic content and each of the three flavonoids. The PCA biplot associated exotic genotypes (‘ML-SF-29′, ‘PS’, ‘TZ-1’, and ‘GPS’) and local genotypes (‘ML-3-KK’, ‘ML-13-SDM’, and ‘ML-12-TMP’) with high Al, Fe, Zn, N, and TPC. Cluster analysis indicated high “distant groups” between exotic and local genotypes of spider plant. These results indicated that some of the local germplasm of spider plant was largely inferior to the exotic germplasm in terms of their mineral composition but contained considerable quantities of quercetin-3-rutinoside, particularly in the local genotypes ‘MP-B-2-CG’ and ‘MP-B-1-CG’. There is a need for genetic improvement of the local germplasm in some of the minerals particularly to benefit the end-users.


Diversity ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 237
Author(s):  
Thomas E. Marler

A long-term reciprocal garden study was used to determine adaptive variation between Cycas micronesica K.D. Hill plants from north versus south Guam. Half-siblings from each location were planted as one-leaf seedlings in north and south gardens and monitored for 15 years. Stem height and diameter, and leaf number and maximum length were measured yearly. Survival and plant size traits were evaluated using a two-way factorial. In both locations, the local genotypes out-performed the foreign genotypes in terms of survival and growth. Survival of the foreign genotypes began to decline by year 4 and was less than 10% by year 15. Survival of the local genotypes was 70% for the north garden and 100% for the south garden. The north site was more hostile to plant performance because overall survival and plant growth were less than for the south site. The most likely environmental factor provoking local adaptation was highly contrasting soil characteristics between north and south Guam. The results indicates that long-term conservation success for C. micronesica and other cycad species must include the concept of local adaptation into decisions for transplantation and restoration projects.


2021 ◽  
Author(s):  
Eduardo Sandoval-Castro ◽  
Ayesha Yolitzín Peraza-Magallanes ◽  
Richard S Dodd ◽  
Vanessa E T M Ashworth ◽  
Abraham Cruz Mendívil ◽  
...  

Abstract The avocado (Persea americana Mill.) is highly valued worldwide for its desirable nutritional properties and broader applications in the oil and pharmaceutical industry. Despite northwestern Mexico, with a tropical semi desert climate, is not considered as part of the avocado origin center in Mexico, it possesses a high morphological diversity of avocado fruits. In an effort to provide more information about the diversity present in this region and at the same time, to support a more efficient production of avocado in this climate, this study characterized the genetic diversity of avocado trees growing at northwestern Mexico. Genetic diversity was estimated by using eight microsatellite loci. 45 seed-derived trees, with contrasting fruit morphology were identified. Results showed a high level of genetic diversity with 11.5 alleles per locus, a polymorphic index content of 0.75, and observed and expected heterozygosity values of 0.58 and 0.79, respectively, these values were similar to those observed in germplasm banks and genotypes from centers of origin. Clustering analysis assigned local genotypes to six clusters but did not provide a clear separation among avocado races, suggesting that local genotypes are a result of racial hybridization. Interestingly, avocados from the Mexican race and the commercial variety Hass clustered into two different groups. Despite not being considered a center of origin, results confirm an extensive diversity in northwestern Mexico, encouraging further exploration and preservation of genotypes with desirable traits to future breeding programs for the selection of local genotypes adapted to a lowland tropical climate.


Author(s):  
M.N. Mamathashree ◽  
S. Shyamalamma

Background: French bean or kidney bean is a rich source of protein, fibers and minerals in diet and is used by poor people in several Asian countries as a cheap source of protein and mineral vegetable/seed grain. Thus, the study on micronutrient variations in French bean local genotypes was undertaken to screen for Fe, Zn, Cu and Mn content in comparison to developed varieties. Methods: Selected twenty local genotypes/accessions and four released varieties of French bean were grown in the field. The morphological characterization was carried out as per crop descriptors. The micronutrient analysis for Fe, Zn, Mn and Cu content was carried out using AAS. Further, accessions were screened using fourteen SSR markers linked to Fe and Zn content.Result: The results revealed that accession IC342273 exhibited significantly higher dry pod (58.94 g) and seed yield (809.74 g) per plot. The accession IC538420 (177.31 ppm) exhibited significantly higher mean Fe content and was on par with Arka Suvidha (176 ppm). Accession EC500226 showed significantly higher Zn (26.91 ppm), Mn (15.31 ppm) and Cu (8.55 ppm) content over other accessions and varieties. Among fourteen SSR primers amplified, two genomic SSR markers (BM154 and BM211) exhibited higher polymorphism among the accessions. The SSR markers classified the accessions and varieties into two clusters, which can be used in crop improvement programmes on French bean for micronutrients bio-fortification studies.


2020 ◽  
Vol 7 (2) ◽  
pp. 53
Author(s):  
Ilham Nur Ardhi Wicaksono ◽  
Budi Martono

<em>The estimated value of genotype variability, phenotype variability, environmental variability, and heritability have an important role in breeding activities of tea</em> <em>in order to create the superior varieties. The objective of this study was to investigate the phenotypic performance, genotype and phenotype variation, and heritability value of nine genotypes of tea. The study was conducted at Pemandangan block, Tambi Plantation Unit,  Wonosobo, Central Java, from April 2013 until April 2014. The Randomized Complete Block Design (RBD) with nine treatments and three replications was used in this study. The 9<sup>th</sup> of treatments consisted of 2 genotypes of tea resulting from a cross (GMB 3 and GMB 4), 2 introduced genotypes (TRI 2024 and TRI 2025), and 5 local genotypes resulting from selection (Cin 143, Kiara 8, RB 3, Tambi 1, and Tambi 2). The results showed that the genotype of GMB 3 more higher than other genotypes in pecco length, leaf length, leaf width, length of leaf stalk, and length of leaf internodes characters, whereas Tambi 2, GMB 3, and GMB 4 more higher in fresh weight of P+2 and P+3. The production of fresh shoot of GMB 4 more higher than the other genotypes, but not significant with GMB 3 and RB 3. The leaf length and fresh shoot production characters has a wide of genotype and phenotype variability, whereas the high of heritability value were found in all characters observed. The selection based on leaf length and fresh shoot production characters will be effective because both characters have a high genotypes variability and heritability.</em>


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