Connectivity of Fennoscandian Shield terrestrial deep biosphere microbiomes with surface communities

The deep biosphere is an energy constrained ecosystem yet fosters diverse microbial communities that are key in biogeochemical cycling. Whether microbial communities in deep biosphere groundwaters are shaped by infiltration of allochthonous surface microorganisms or the evolution of autochthonous species remains unresolved. In this study, 16S rRNA gene amplicon analyses showed that few groups of surface microbes infiltrated deep biosphere groundwaters at the Äspö Hard Rock Laboratory, Sweden, but that such populations constituted up to 49% of the microbial abundance. The dominant persisting phyla included Patescibacteria, Proteobacteria, and Epsilonbacteraeota. Despite the hydrological connection of the Baltic Sea with the studied groundwaters, infiltrating microbes predominantly originated from deep soil groundwater. Most deep biosphere groundwater populations lacked surface representatives, suggesting that they have evolved from ancient autochthonous populations. We propose that deep biosphere groundwater communities in the Fennoscandian Shield consist of selected infiltrated and indigenous populations adapted to the prevailing conditions.

Design of a Capacitance-to-Digital Converter Based on Iterative Delay-Chain Discharge in 180 nm CMOS Technology

The design of advanced miniaturized ultra-low power interfaces for sensors is extremely important for energy-constrained monitoring applications, such as wearable, ingestible and implantable devices used in the health and medical field. Capacitive sensors, together with their correspondent digital-output readout interfaces, make no exception. Here, we analyse and design a capacitance-to-digital converter, based on the recently introduced iterative delay-chain discharge architecture, showing the circuit inner operating principles and the correspondent design trade-offs. A complete design case, implemented in a commercial 180 nm CMOS process, operating at 0.9 V supply for a 0–250 pF input capacitance range, is presented. The circuit, tested by means of detailed electrical simulations, shows ultra-low energy consumption (≤1.884 nJ/conversion), excellent linearity (linearity error 15.26 ppm), good robustness against process and temperature corners (conversion gain sensitivity to process corners variation of 114.0 ppm and maximum temperature sensitivity of 81.9 ppm/∘C in the −40 ∘C, +125 ∘C interval) and medium-low resolution of 10.3 effective number of bits, while using only 0.0192 mm2 of silicon area and employing 2.93 ms for a single conversion.

Improved uplink throughput and energy efficiency of LoRaWAN using 2-hop LEACH protocol

The low power wide area networks (LPWAN) is the new connectivity technology that is geared towards energy constrained internet of things (IoT) devices, is starting to become one of the drivers of the re-accelerating IoT market and has one goal: ensure the wide range distance while reducing the battery energy consumption. We focus in this paper on the evaluation of the uplink throughput of the long-range wide area networks (LoRaWAN) then we attempt optimize the throughput and power dissipation using low energy adaptive clustering hierarchy (LEACH) protocol. Therefore, we exploit a novel module developed in NS-3 simulator for obtaining the first measurements scenario, then the LEACH algorithm for the second optimization case. As result, the simulation analysis will help us to add a new LoRaWAN routing protocol feature.