perfringolysin o
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2022 ◽  
Vol 3 (1) ◽  
pp. 101068
Author(s):  
Leire Goicoechea ◽  
Fabian Arenas ◽  
Fernanda Castro ◽  
Susana Nuñez ◽  
Sandra Torres ◽  
...  

2021 ◽  
Author(s):  
Conall Mc Guinness ◽  
James Walsh ◽  
Charles Bayly-Jones ◽  
Michelle Dunstone ◽  
Craig Morton ◽  
...  

The cholesterol-dependent cytolysin perfringolysin O (PFO) is secreted by Clostridium perfringens as a bacterial virulence factor able to form giant ring-shaped pores that perforate and ultimately lyse mammalian cell membranes. To resolve the kinetics of all steps in the assembly pathway, we have used single-molecule fluorescence imaging to follow the dynamics of PFO on dye-loaded liposomes that lead to opening of a pore and release of the encapsulated dye. Formation of a long-lived membrane-bound PFO dimer nucleates the growth of an irreversible oligomer. The growing oligomer can insert into the membrane and open a pore at stoichiometries ranging from tetramers to full rings (~35-mers), whereby the rate of insertion increases linearly with the number of subunits. Oligomers that insert before the ring is complete continue to grow by monomer addition post insertion. Overall, our observations suggest that PFO membrane insertion is kinetically controlled.


Author(s):  
Moustafa Elkalaf ◽  
Karolína Vaněčková ◽  
Pavla Staňková ◽  
Zuzana Červinková ◽  
Jan Polák ◽  
...  

2021 ◽  
Author(s):  
Michael J Senior ◽  
Carina Monico ◽  
Eve E Weatherill ◽  
Robert Gilbert ◽  
Alejandro Heuck ◽  
...  

We exploit single-molecule tracking and optical single channel recording in droplet interface bilayers to resolve the assembly pathway of the Cholesterol-Dependent Cytolysin, Perfringolysin O. This enables quantification of the stoichiometry of PFO complexes during assembly with millisecond temporal resolution and 20 nanometre spatial precision. Our results support a model of overall stepwise irreversible assembly, dominated by monomer addition, but with infrequent assembly from larger partial complexes. Furthermore, our results suggest a dominant proportion of inserted, but non-conductive intermediates in assembly.


2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Takayuki Matsumura ◽  
Ayae Nishiyama ◽  
Michio Aiko ◽  
Akira Ainai ◽  
Tadayoshi Ikebe ◽  
...  

2020 ◽  
Vol 11 ◽  
Author(s):  
Jian Zhang ◽  
Shui Liu ◽  
Lining Xia ◽  
Zhongmei Wen ◽  
Naiyu Hu ◽  
...  

2020 ◽  
Vol 11 ◽  
Author(s):  
Shui Liu ◽  
Xiaofeng Yang ◽  
Hong Zhang ◽  
Jian Zhang ◽  
Yonglin Zhou ◽  
...  

RSC Advances ◽  
2020 ◽  
Vol 10 (63) ◽  
pp. 38678-38682
Author(s):  
Aleksandra Šakanović ◽  
Nace Kranjc ◽  
Neža Omersa ◽  
Marjetka Podobnik ◽  
Gregor Anderluh

By using developed ribosomal display, we discovered variants of perfringolysin O, a pore forming toxin from bacteria Clostridium perfringens, with non-conserved amino acid substitutions at regions crucial for cholesterol recognition.


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