morphological index
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2021 ◽  
Vol 33 (3) ◽  
pp. 187
Author(s):  
Natalia Tanojo ◽  
Damayanti Damayanti ◽  
Budi Utomo ◽  
Evy Ervianti ◽  
Dwi Murtiastutik ◽  
...  

Background: Leprosy is a neglected tropical disease caused by chronic granulomatous infection of Mycobacterium leprae. Indonesia ranks third in new case findings, with 84% of the case being multibacillary (MB) leprosy. MB leprosy cases have a higher risk of leprosy reactions and physical disabilities that decrease quality of life. Purpose: To determine the demographic, clinical characteristics, and white blood analysis of newly diagnosed MB leprosy patients, especially concerning leprosy reactions. Methods: This is a descriptive retrospective study with a cross-sectional design that describe the following data: domicile, gender, age, treatment status, disabilities, body mass index (BMI); bacterial index (BI), morphological index (MI), white blood cell (WBC) and differential counts, and thrombocyte count. Result: This study included 176 adult MB cases, predominantly male aged 20–39 years old with average BMI, lived in Surabaya with negative history of multi-drug therapy, disability, BI, nor MI. The grade 2 disability (G2D) percentage in this study setting than in Indonesia (10.7% vs. 6.43%). The WBCs, especially neutrophil count, was higher in T2R group. Monocyte and lymphocyte counts were relatively similar. There was an increase in thrombocyte count in leprosy reaction groups. Conclusion: MB leprosy in the endemic area, which is more commonly found in productive-aged male, displayed higher G2D than global Indonesia population. Thus denotes the importance of active case findings. The difference in blood analysis characteristics between MB leprosy with and without reactions may serve as the foundation for future study.


2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Yan Liu ◽  
Yu Zhang ◽  
Yueguo Chen

Abstract Background In vivo corneal biomechanics evaluation has been used to help screen early keratoconus in recent years. This study is to evaluate the value of a Scheimpflug-based biomechanical analyser combined with tomography in detecting subclinical keratoconus by distinguishing normal eyes from frank keratoconus (KC) and forme frusta keratoconus (FFKC) eyes in Chinese patients. Methods Study design: diagnostic test. This study included 31 bilateral frank keratoconus patients, 27 unilateral clinically manifesting keratoconus patients with very asymmetric eyes, and 79 control subjects with normal corneas. Corneal morphological and biomechanical parameters were measured using a Pentacam HR and a Corvis ST (OCULUS, Wetzlar, Germany). The diagnostic ability of computed parameters reflecting corneal biomechanical and morphological traits [including the Belin-Ambrósio deviation index (BAD_D), the Corvis biomechanical index (CBI) and the tomographic and biomechanical index (TBI)] was determined using receiver operating characteristic (ROC) curve analysis and compared by the DeLong test. Additionally, the area under the curve (AUC), the best cut-off values, and the Youden index for each parameter were reported. A novel corneal stiffness parameter, the stress-strain index (SSI), was also compared between KC, FFKC and normal eyes. Results Every morphological and biomechanical index analysed in this study was significantly different among KC, FFKC and normal eyes (P = 0.000). The TBI was most valuable in detecting subclinical keratoconus (FFKC eyes), with an AUC of 0.928 (P = 0.000), and both forms of corneal ectasia (FFKC and frank KC eyes), with an AUC of 0.966 (P = 0.000). The sensitivity and specificity of the TBI was 97.5 and 77.8 % in detecting FFKC and 97.5 and 89.7 % in detecting any KC, respectively, with a cut-off value of 0.375. The morphological index BAD_D and the biomechanical index CBI were also very useful in distinguishing eyes with any KC from normal eyes, with AUCs of 0.965 and 0.934, respectively. The SSI was significantly different between KC, FFKC and normal eyes (P = 0.000), indicating an independent decrease in corneal stiffness in KC eyes. Conclusions The combination of a Scheimpflug-based biomechanical analyser and tomography could increase the accuracy in detecting subclinical keratoconus in Chinese patients. The TBI was the most valuable index for detecting subclinical keratoconus, with a high sensitivity and specificity. Evaluation of corneal biomechanical properties in refractive surgery candidates could be helpful for recognizing potential keratoconic eyes and increasing surgical safety.


Author(s):  
Ymberzal Koul ◽  
V. Harshini ◽  
S.M.K. Karthickeyan ◽  
K. Thilak Pon Jawahar ◽  
A. Gopinathan

Background: Nattukuttai is a small-sized cattle population, native to north-eastern districts of Tamil Nadu. In light of the ongoing research on genetic characterisation of cattle genetic resources of India, the present study was undertaken with the objective of cytogenetic characterisation of Nattukuttai cattle, which is imperative for its conservation and genetic implications to breeding programs. Methods: Blood samples from ten Nattukuttai cattle (five males and five females) were utilized to study the chromosome profile through short-term lymphocyte culture method. Good metaphase spreads were selected for estimation of the relative length, arm ratio, centromeric index and morphological index. Result: The diploid number was 60. All the 29 pairs of autosomes and Y-chromosome were acrocentric while X-chromosome was sub-metacentric. The mean relative length of autosomes ranged from 5.24 ±0.08 to 1.90±0.06. X-chromosome was the largest in the karyotype (5.64±0.12), while the Y-chromosome was the smallest (1.85±0.03). The arm ratio, centromeric index and morphological index were 1.98±0.02, 0.33±0.03 and 4.06±0.4 respectively. The study revealed that the chromosome architecture of Nattukuttai cattle was similar to that of other breeds of Zebu cattle.


Agriculture ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 770
Author(s):  
Chuanhai Shu ◽  
Feijie Li ◽  
Dongdong Liu ◽  
Jian Qin ◽  
Mingtian Wang ◽  
...  

Productive tiller percentage (PTP) is the only available comprehensive indicator of rice population quality. However, productive panicle number (PN) has a great effect on its characterization accuracy. Panicle exsertion is an important but difficult to describe morphological index; therefore, it cannot be easily determined. The aims of this study were to develop heading uniformity (HU), which describes the difference in the degree of rice panicle exsertion, as a new comprehensive indicator by designing a representative sampling and calculation method and exploring the relationship between HU and yield components. HU first decreased then increased after initial heading, exhibiting a single-valley curve. Adequate HU was obtained by panicle sampling on day two or three (panicle N fertilizer proportion ≤40 or >40%) after initial heading. The explanatory power of PTP for grain yield variance was markedly insufficient in low- and high-PN rice populations. Compared with the percent contribution of PTP to grain yield variance (12.32–41.26%), that of HU (49.02–61.93%) was greater and more stable across rice populations of different PNs. Moreover, HU showed fewer interannual variations, despite large interannual differences in weather and soil conditions. Hence, HU may have applications as a comprehensive indicator of rice population quality.


Author(s):  
Basavraj Inamdar ◽  
R. Nagaraja ◽  
H.M. Yathish ◽  
S. Naveen Kumar ◽  
G.S. Naveen Kumar ◽  
...  

Background: To characterize the chromosomal complement of Nandidurga goats. Methods: Blood samples for short term lymphocyte culture were collected from 5 bucks and 5 does from its breeding tract and then mitotic chromosomal spreads were accomplished. Result: The diploid chromosome number was found to be 60, consisting of 58 acrocentric autosomes and 2 sex chromosomes (X and Y). The X chromosome was found to be the longest acrocentric and Y chromosome was found to be sub metacentric. The mean mitotic drive was 64.5±2.01 and 63.15±1.30 percent in bucks and does, respectively. The mean relative length of autosomes varied from 2.08±0.24 to 4.81±0.12 in does and 2.09±0.09 to 4.70±0.11in bucks. The relative length of X chromosome in does was 5.14±0.17 and that in bucks was 4.92±0.21, whereas Y chromosome had a relative length of 1.99±0.20. The mean Arms Ratio, Centromeric Index and Morphological Index were 2.47, 31.62 and 396.14, respectively. This cytogenetic analysis indicates the normal chromosomal complement in the studied Nandidurga goats.


2021 ◽  
Author(s):  
Wei Feng ◽  
Meng Zhen ◽  
Wei Ding ◽  
Qishu Zou

Abstract The inadequate consideration of the impact of building morphology on ventilation efficiency in many urban residential areas has resulted in a series of environmental problems that threaten human health. The purpose of this paper is to establish a prediction model between ventilation efficiency and building forms in residential areas. Firstly, the characteristics of vertical wind profile in residential areas are measured through unmanned aerial vehicle (UAV); secondly, the wind speed ratio (WSR) at different height levels under the impact of morphological index (floor area ratio, building density, average building height, enclosure degree, height fall and maximum building height) in the residential area is simulated by ENVI-met; finally, two kinds of prediction formulas are obtained: (1) the average ventilation efficiency at the pedestrian level and (2) the prediction formula of WSR at different heights. The results show that the wind speed (WS) in residential area below 35 m is about 0.6 m/s lower than that in park. The results of numerical simulation show that the mean WSR at the pedestrian level is negatively correlated with each index and the height fall morphological index has the greatest impact on the WSR at different heights. The research can provide a reference for the optimal planning and design of ventilation efficiency of residential buildings, especially those in static wind areas.


Animals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 925
Author(s):  
Maria Teresa Antognoni ◽  
Maria Luisa Marenzoni ◽  
Ambra Lisa Misia ◽  
Luca Avellini ◽  
Elisabetta Chiaradia ◽  
...  

Storage lesions (SLs) occur when the red blood cell quality is altered during the preservation of blood units. Pre-storage leukoreduction would limit the number of SLs. The aims of this study were to evaluate the effectiveness of a leukoreduction filter for human use and the effect of pre-storage leukoreduction on some ematobiochemical parameters in stored canine whole blood. Seven canine blood units were tested. Each one was divided into two units—one leukoreduced (LRWB) and one non-leukoreduced (nLRWB). On each unit, we determined the complete blood count (CBC), lactate-dehydrogenase (LDH), electrolytes (Na+, K+, Cl−), morphological index (MI) and hemolysis, on storage days 0, 7, 14, 21, 28, 35, and 42. Leukoreduction allowed a 98.30% recovery of the RBC count, retaining 99.69% and 94.91% of WBCs and PLTs, respectively. We detected a significant increase of LDH and MI with strongly higher values in nLRWB compared to LRWB. A progressive increase in electrolytes and LDH concentrations was observed as indices of stored hemolysis. LDH showed significantly lower values in LRWB units compared to nLRWB, suggesting its release from leukocytes. In the majority of units, hemolysis reached 1% on the 42nd day of storage. We assert the human leukoreduction filter effectiveness on canine whole blood, and we recommend using nLRWB before day 14, especially for critically ill patients. The difference of the basal hemolysis (day 0) percentages observed between subjects suggests that more studies should be performed to confirm a possible inter-individual donor biological variability of RBC membrane resistance, as happens in humans.


2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Rong Yuan ◽  
Yuefen Cao ◽  
Tengyu Li ◽  
Feng Yang ◽  
Li Yu ◽  
...  

AbstractBackgroundCotton stem trichomes and seed fibers are each single celled structures formed by protrusions of epidermal cells, and were found sharing the overlapping molecular mechanism. Compared with fibers, cotton stem trichomes are more easily observed, but the molecular mechanisms underlying their development are still poorly understood.ResultsIn this study,Gossypium hirsutum(Gh) andG. barbadense(Gb)were found to differ greatly in percentages of varieties/accessions with glabrous stems and in trichome density, length, and number per trichopore.Ghvarieties normally had long singular and clustered trichomes, whileGbvarieties had short clustered trichomes. Genetic mapping using five F2populations from crosses between glabrous varieties and those with different types of stem trichomes revealed that much variation among stem trichome phenotypes could be accounted for by different combinations of genes/alleles on Chr. 06 and Chr. 24. The twenty- six F1generations from crosses between varieties with different types of trichomes had varied phenotypes, further suggesting that the trichomes of tetraploid cotton were controlled by different genes/alleles. Compared to modern varieties, a greater proportion ofGhwild accessions were glabrous or had shorter and denser trichomes; whereas a smaller proportion ofGbprimitive accessions had glabrous stems. A close correlation between fuzz fiber number and stem trichome density was observed in bothGhandGbprimitive accessions and modern varieties.ConclusionBased on these findings, we hypothesize that stem trichomes evolved in parallel with seed fibers during the domestication of cultivated tetraploid cotton. In addition, the current results illustrated that stem trichome can be used as a morphological index of fiber quality in cotton conventional breeding.


2021 ◽  
Author(s):  
RUI-QI DONG ◽  
TAO-PING BAI ◽  
YANG LIU

Abstract Aim: After limb amputation, patients will face many complex postoperative problems. The pathogeny of these problems usually involves blood circulation disorders, vascular lesions and other angioneurotic issues. The correlation between blood flow/vessels and those residual limb problems like the most prevalent muscle atrophy is very importent. Method: Through the case study of eight unilateral trans-femoral amputees, this research performed the morphological index analysis of residual limb muscle atrophy, arterial shrinkage and their potential correlations. Results: The atrophy level of muscles and the shrinkage level of arteries were different. Overall, Different muscles have different atrophy. Compared with the prosthesis users, those cases without using prosthesis exhibited more severe muscle atrophy and arterial shrinkage in their residual limbs. For the correlation between muscle atrophy and arterial shrinkage on the basis of blood supply relationship, there was a positive correlation expressed only in the cases using prosthesis. Conclusion: Using a prosthesis could not only effectively achieve the functional compensation for residual limb, but also promote a joint adaptation of the muscular and arterial physiological state of both the sound and amputated limbs to the new gait pattern and body balance, as well as rehabilitation process.


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