Characteristics and possible mechanisms of formation of microinversions distinguishing human and chimpanzee genomes

Genomic inversions come in various sizes. While long inversions are relatively easy to identify by aligning high-quality genome sequences, unambiguous identification of microinversions is more problematic. Here, using a set of extra stringent criteria to distinguish microinversions from other mutational events, we describe microinversions that occurred after the divergence of humans and chimpanzees. In total, we found 59 definite microinversions that range from 17 to 33 nucleotides in length. In majority of them, human genome sequences matched exactly the reverse-complemented chimpanzee genome sequences, implying that the inverted DNA segment was copied precisely. All these microinversions were flanked by perfect or nearly perfect inverted repeats pointing to their key role in their formation. Template switching at inverted repeats during DNA replication was previously discussed as a possible mechanism for the microinversion formation. However, many of definite microinversions found by us cannot be easily explained via template switching owing to the combination of the short length and imperfect nature of their flanking inverted repeats. We propose a novel, alternative mechanism that involves repair of a double-stranded break within the inverting segment via microhomology-mediated break-induced replication, which can consistently explain all definite microinversion events.

Additional opportunities for using computer technologies in expert analysis of laboratory data

The presented work reflects the author’s algorithm of mathematical processing and possible interpretations of the results obtained when creating expert analytical systems using computer technology. As initial parameters for the construction of neural networks of the second level, it is proposed to use individual results of calculations of the ratios of a number of laboratory indicators capable of forming a single functional cluster (leukogram, indicators of water-electrolyte balance, protein fractions, etc.). Further, using the proposed calculation algorithm and the archive database, structural features in the ′deformation′ of the ratio panel were compared against the background of the growth of each indicator, a matrix table was built reflecting the degree (correlation coefficients, KCr) of the coincidence of the features of the formation of the structure of the obtained panels of the parameters determined in individual observations. At high values of KCr, a conclusion is made about their (determined parameters) unified participation in the mechanism of emerging disorders. Thus, the system allows you to establish a leading complex of associated relationships by changes in the structure of ratios in the selected panel, corresponding to the dynamics of deviation of the target (analyzed) indicator, thereby differentiating the different mechanisms of formation of pathological deviations in this patient, the manifestation and balance of their values in the system-wide response, to evaluate the specific value of the absolute indicator as the final result of such interaction at the time of examination of the patient. The approach used, proposed by the authors, allows us to evaluate not only quantitative relationships, but also the features of the manifestation of the functional properties of the evaluated indicators. All of the above significantly expands the informativeness of the obtained laboratory data, allowing us to build a reasonable paradigm of the connections of the emerging complex of pathological disorders in each individual case. In the list of references, the authors cite publications as concrete examples of the use of the proposed approach in assessing a complex of disorders with different pathological processes using different panels in calculations.

Review on Acrylamide: A Hidden Hazard in Fried Carbohydrate-Rich Food

Acrylamide is classified as a hazard whose formation in carbohydrate-rich food cooked at a high temperature has created much interest in the scientific community. The review attempts to comprehend the chemistry and mechanisms of formation of acrylamide and its levels in popular foods. A detailed study of the toxicokinetic and biochemistry, carcinogenicity, neurotoxicity, genotoxicity, interaction with biomolecules, and its effects on reproductive health has been presented. The review outlines the various novel and low-cost conventional as well as newer analytical techniques for the detection of acrylamide in foods with the maximum permissible limits. Various effective approaches that can be undertaken in industries and households for the mitigation of levels of acrylamide in foods have also been discussed. This review will assist to provide in depth understanding about acrylamide that will make it simpler to assess the risk to human health from the consumption of foods containing low amounts of acrylamide.

Covid-19: Systems Transdisciplinary Generalization, Technical and Technological Ideas, and Solutions

Aim/Purpose: The Covid-19 pandemic has created many adverse effects. It overloads the healthcare system, causes deaths, and angers some at anti-covid restrictions. This study examines the feasibility of using technical and technological ideas to overcome these effects. The solution is based on new knowledge about the virus, its nature, formation, and activation in the environment. Background: The rapid spread of a new coronavirus infection is taking place against the background of a lack of time required to create new treatment scenarios for the disease, development, production, and vaccine safety research. In such a situation, it became necessary to gain this time for organizing and conducting events that could reduce the burden on the healthcare system. Methodology: The science that studies the morphology, physiology, genetics, ecology, and evolution of viruses is virology. The modern development of virology is moving towards a more accurate and comprehensive description of the mechanisms of interaction of viruses with the host organism. This contributed to the emergence of genomics, transcriptomics, proteomics, and immunomics. However, in virology, there is no particular discipline that sets itself three fundamental goals: to substantiate a single concept of the emergence of viruses; to study the natural mechanisms of formation of virus molecules in the environment; to describe the natural mechanisms of activation of certain viruses in the environment that cause viral pandemics. As a result, there are many articles among the published scientific articles on viruses dealing with the mechanisms of interaction of viruses with the host organism. However, there are no articles on the natural mechanisms of formation and activation of certain viruses in the environment. In the absence of such specialized articles, we were forced to use the method of systems transdisciplinary generalization of disciplinary knowledge to achieve our article’s purpose. Generalization created new knowledge about the nature of viruses, about the mechanisms of their formation and activation in the environment and cells of biological organisms. It is logical to assume that to synchronize the state of biological objects of all functional ensembles on the planet, it is necessary to create and activate appropriate “technological tools.” We have suggested and proved that RNA viruses play the role of such tools. Piezoelectricity activates viruses. It occurs during the compression and stretching of sedimentary rocks and bases of continental plates in different territories. Contribution: The systems transdisciplinary generalization of the knowledge of scientific disciplines made it possible to edit the concept of viruses, to eliminate stereotypes that arose due to the use of unsuccessful analogies. As a result of this generalization, it was possible to prove that viruses are not intracellular parasites. The virus is a “technological tool” of the planetary organizing component. This “tool” aims to correct the genetic programs of organisms of all functional ensembles (plants, animals, people), which will maintain the state of organisms and the parameters of their metabolism in changing environmental conditions. Findings: The viruses that triggered pandemics in the 20th century and early 21st century are RNA viruses. RNA molecules play the role of “technological tools” that the planetary organizing component uses to carry out short-term and long-term adjustments and constant support of the genetic programs of biological organisms. Therefore, in such a situation, it is advisable to talk not about the fight against the virus but only about eliminating the negative manifestations of the Covid-19 pandemic: reducing the number of people in need of emergency hospitalization, eliminating cases of the acute course of the disease and deaths. It is proposed to use certain technical and technological ideas and solutions to eliminate these negative manifestations. Recommendation for Researchers: This paper recommends that researchers use new interdisciplinary and transdisciplinary approaches. They challenge assumptions and conclusions about the nature of viruses, and the mechanisms of their formation and activation in the environment can initiate. Such new research might describe the mechanisms that form and activate viruses in the environment and the body’s cells. They also might provide practical use of this knowledge to eliminate the multiple speculations and fears that arise against the background of reports of the likely appearance of more deadly viruses and viral infections. Future Research: The results of a systems transdisciplinary generalization of disciplinary knowledge about the nature and purpose of viruses are essential for expanding the horizon of the scientific worldview. Future fundamental research on the mechanisms of objective organizing constituents, a general description given in this article, will contribute to a deeper understanding of chemical and biological evolution mechanisms in which modern humanity is involved. In due time, such an understanding will allow a new look at the existing scenarios of the world socio-economic order, explore and describe new principles of sustainable development of society.

INDICATIVE APPROACH AS A MEANS OF INFLUENCING THE CORPORATE CULTURE OF AN EDUCATIONAL ORGANIZATION ON THE FORMATION OF THE PROFESSIONAL IDENTITY OF TEACHERS

The problem considered in this article is to determine the role of the corporate culture of educational organizations in the process of forming the professional identity of teachers. Emphasis is placed on the indicative approach, within the framework of which the potential of teachers is supported; detection by teachers of their own problems and giving them a developmental character by turning the problem into a task of activity. It has been determined that the indicative approach brings closer to understanding the mechanisms of formation of new subjective qualities of teachers in the process of putting forward and achieving goals at the stages of mastering certain types of activities, as a result, the development of the fragmentary experience of teachers into a stable motivation for professional improvement in the process of collective activity.

Principal stages of speech pragmasemantic sense formation and methods of its analysis

The article is devoted to peculiarities and differences of two linguistic fields semantics and pragmatics. The objective of the work is to state the extent of participation of semantic and pragmatic parameters in the process of sense formation. The relevance of the study is beyond doubt, since the pragmasemantic analysis of discourse functional space helps to identify the potential of linguistic means in the process of forming meanings and their transformation into integral sense under the influence of contextual factors, and to determine optimal ways of expressing communicative intention of the author of the discourse and of providing adequate understanding of the sense by the referent. The author also describes main features, differences and similarities of the concepts text and discourse. The specificity of semantic and pragmatic meanings, their correlation and the degree of participation in formation of discourse functional space are analyzed. On the basis of this, the algorithm for pragmasemantic sense formation is deduced. The author emphasizes that the initial meaning is formed by a semantic meaning, in the process of speech actualizing it is supplemented with a pragmatic meaning, which is further transformed into a pragmatic and semantic and communicative-pragmatic sense. This process is illustrated by fragments from works of fiction by contemporary English-speaking authors. The research uses semantic and pragmatic analysis and discourse analysis as the principal methods. The author describes their features, and proves the viability of their application to the object of the research. The author comes to the conclusion that the pragmasemantic analysis of English discourse makes it possible to understand the mechanisms of formation of meanings and sense of a speech work. The contribution of both semantics and pragmatics to the overall communicative interaction is undeniable, since any communication is necessarily analyzed taking into account semantic and pragmatic aspects.

Social Networks in Modern Russia: Scale, Structure and Functioning Mechanisms

In the article, based on the data of the Institute of Sociology of the FCTAS RAS in 2021, it is shown that most representatives of mass segments of the population have access to resource exchange in social networks and only 16% of them are completely excluded from it. It is also demonstrated that the resource potential of the networks of representatives of these layers is in most cases limited to simple monetary types of help. At the same time, the structure of the networks is asymmetric – there are fewer donors in them than recipients. It is shown that among the participants of social networks there are “pure recipients” who receive aid, but do not provide it, and donors are heterogeneous in their resource potential, and three quarters of them have it at a very low level. It is also recorded that the basis of resource exchange in Russian networks is approximately 7% of the population, which can be described as high-resource donors. They not only help others, but also receive help from networks, moreover, they are the main beneficiaries of membership in networks. As a result of this structure and principles of functioning, social networks tend to deepen social inequalities rather than smooth them out. The mechanisms of formation and functioning of social networks are related to the opportunities for close communication of their participants and their lifestyle in general. The pandemic had a positive impact on mutual help in Russian society, which was expressed in increasing access to resource exchange in networks, converting it into real help and expanding the prevalence of previously atypical forms of social network development for post-Soviet Russia.

Analysis of Russian dissertation research on wrong behaviour from the standpoint of the theory of sets by Dimitri Uznadze modified by Aleksandr Asmolov

In the review article, in the context of discussing the current problem of clarifying the causes of wrong behaviour and developing approaches to its correction based on the analysis of literature sources; the hypothesis is substantiated that behavioural errors in all their diversity are a consequence of the actualisation of sets at different levels of organisation. The scientific novelty of the research is in the fact that in order to achieve this goal, a comparative analysis of wrong behavior has been carried for the first time out from the standpoint of the classical theory of sets by Dimitri Uznadze and from the standpoint of modification of this theory by Aleksandr Asmolov, according to which human activity is stabilised by three types of sets at different levels of organization – operational, purpose and semantic. For the factual argumentation of the results of the analysis, all dissertations (46) that had been defended in Russia over the past 30 years as part of the study of wrong behaviour were studied. In these works, errors are described and discussed, which can be conditionally subdivided into three main large groups – automatic errors, errors of failure to achieve the goal and errors of the meaning of activity. These groups of errors have signs of level sets, and that confirms the hypothesis formulated in this article. The development of approaches to the correction of wrong behaviour on the basis of psychological mechanisms of formation and correction of sets at different levels of organisation within the framework of the concept of set activity by Aleksandr Asmolov.

Oxidative Crosslinking of Peptides and Proteins: Mechanisms of Formation, Detection, Characterization and Quantification

Covalent crosslinks within or between proteins play a key role in determining the structure and function of proteins. Some of these are formed intentionally by either enzymatic or molecular reactions and are critical to normal physiological function. Others are generated as a consequence of exposure to oxidants (radicals, excited states or two-electron species) and other endogenous or external stimuli, or as a result of the actions of a number of enzymes (e.g., oxidases and peroxidases). Increasing evidence indicates that the accumulation of unwanted crosslinks, as is seen in ageing and multiple pathologies, has adverse effects on biological function. In this article, we review the spectrum of crosslinks, both reducible and non-reducible, currently known to be formed on proteins; the mechanisms of their formation; and experimental approaches to the detection, identification and characterization of these species.