Impedance Flow Cytometry for Selection of Pollen Traits Under High Temperature Stress in Pepper

High temperature stress is a major limiting factor for pepper productivity, which will continue to be a problem under climate change scenarios. Developing heat tolerant cultivars is critical for sustained pepper production, especially in tropical and subtropical regions. In fruiting crops, like pepper, reproductive tissues, especially pollen, are the most sensitive to high temperature stress. Typically, pollen viability and germination are assessed through staining and microscopy, which is tedious and potentially inaccurate. To increase efficiency in assessing pollen traits of pepper, the use of impedance flow cytometry (IFC) has been proposed. We conducted three independent experiments to determine the most effective methodology to use IFC for evaluating pollen traits for heat tolerance in pepper. Seven floral developmental stages were evaluated, and stages 3, 4, and 5 were found to best combine high pollen concentration and activity. Flowers in development stages 3, 4, or 5 were then heat treated at 41, 44, 47, 50, and 55 °C or not heat treated (control). The critical temperature to assess heat tolerance using IFC was found to be 50 °C, with a reduction in pollen activity and concentration occurring at temperatures greater than 47 °C. Twenty-one entries of pepper were then accessed for pollen traits using the staining and IFC methods over 2 months, April (cooler) and June (hotter). Growing environment was found to be the greatest contributor to variability for nearly all pollen traits assessed, with performance during June nearly always being lower. PBC 507 and PBC 831 were identified as being new sources of heat tolerance, based on using IFC for assessing pollen. Pollen viability determined by staining and pollen activity determined using IFC were significantly positively correlated, indicating that IFC is an efficient and accurate method to assess pollen traits in pepper. This work provides a basis for further research in this area and supports more efficient breeding of heat-tolerant cultivars.

Palau’s warmest reefs harbor a thermally tolerant coral lineage that thrives across different habitats

Ocean warming is killing corals, but heat-tolerant populations exist; if protected, they could replenish affected reefs naturally or through restoration. Palau’s Rock Islands experience chronically higher temperatures and extreme heatwaves, yet their diverse coral communities bleach less than those on Palau’s cooler outer reefs. Here, we combined genetic analyses, bleaching histories and growth rates of Porites cf. lobata colonies to identify thermally tolerant genotypes, map their distribution, and investigate potential growth trade-offs. We identified four P cf. lobata genetic lineages. On Palau’s outer reefs, a thermally sensitive lineage dominates. The Rock Islands harbor two lineages with enhanced thermal tolerance and no consistent growth trade-off. One of these lineages also occurs on several outer reefs. This suggests that the Rock Islands provide naturally tolerant larvae to neighboring areas. Finding and protecting such sources of thermally-tolerant corals is key to reef survival under 21st century climate change.

Genetic study of terminal heat stress in indigenous collections of Indian mustard (Brassica juncea L.) germplasm

Aim: The present study was carried out to evaluate 145 genotypes of Indian mustard for terminal heat stress under late sown irrigated conditions. Methodology: One hundred and forty five genotypes of Indian mustard were evaluated for terminal heat tolerance, along with six checks in augmented block design. The effect of heat stress was assessed on different agro-morphological traits. Data were recorded on five randomly selected plants for 12 different quantitative traits for each genotype. Results: Terminal heat stress caused significant reduction in performance of all traits, except oil content and fruiting zone length. Seed yield per plant showed highest reduction (33.92%) followed by 1000-seed weight (21.28%). Thousand-seed weight and secondary branches per plant were found associated with seed yield under late sown conditions. Based on yield under stress, yield index, geometric mean productivity and stress tolerance indices, five lines viz., DRMR-59, DRMR-2094, DRMR-2129, DRMR-2071, DRMR-2136 were identified as high yielding and terminal heat stress tolerant. Whereas, based on heat stress susceptibility index, DRMR-1347, DRMR-1154, DRMR-1190 were top performing lines tolerant to terminal heat stress. Interpretation: Selection of high yielding lines under stress condition can be done using correlated traits. Yield under stress was found significantly correlated with YSI, YI, MP, STI and GMP suggesting to use these indices for selection of high yielding and heat tolerant lines. However, further re-validation is very much needed for effective use of these indices for selection of heat tolerant lines in Indian mustard.

Identification of Heat Tolerant Cotton Lines Showing Genetic Variation in Cell Membrane Thermostability, Stomata, and Trichome Size and Its Effect on Yield and Fiber Quality Traits

Heat stress in cotton reduces its productivity. The development of heat-tolerant cotton varieties having resilience against changing climate is feasible. The purpose of this study was to probe the genetic variability in upland cotton for heat tolerance, the association of cell membrane thermostability (CMT), stomata, and trichome size with cotton adaptation to high temperature and effective breeding strategy to advance the valued traits. Relative cell injury percentage (RCI%) in studied genotypes ranged from 39 to 86%. Seventeen genotypes were found heat tolerant on the basis of low RCI%, heat susceptibility index (HSI<1), higher number of boll/plant, and seed cotton yield (SCY). Scanning electron microscopy (SEM) of heat-tolerant genotypes revealed the presence of different size of stomata (21.57 to 105.04 μm2) and trichomes (177 to 782.6 μm) on leaves of selected genotypes. The regression analysis showed a strong and negative association of RCI% and stomata size with SCY. However, no association was observed between the trichome size, yield, and fiber traits. On the overall location basis, a significant genotype × environment interaction was observed. All selected genotypes produced a higher SCY as compared with check varieties. But the stability analysis showed that the high yielding genotypes NIA-M-30, NIA-80, NIA-83, and CRIS-342 were also wide adaptive with unit regression (bi∼1) and non-significant deviation from the regression line (S2d∼0). The ability for the combination of some heat-tolerant genotypes was estimated by using the line × tester method among nine hybrids along with their 3 testers (i.e., male) and 3 lines (i.e., females). Genotypes, CRIS-342 and NIA-Perkh, were observed as best general combiners for SCY with a negative general combining ability effects for RCI%. Five hybrids showed a positive specific combining ability and heterotic effects for studied traits and also found lowest for HSI. RCI% and SCY/plant displayed higher estimates of heritability and genetic advance, indicating the heritability due to additive gene effects and chances of effective selection. The identified heat-tolerant and wide adaptive germplasm can be further advanced and utilized in cotton breeding programs for developing heat-tolerant cultivars. Selection criteria involving CMT and stomata size concluded to be an effective strategy for the screening of heat-tolerant cotton.

Artificial Shading Can Adversely Affect Heat-tolerant Lettuce Growth and Taste, with Concomitant Changes in Gene Expression

Shading has been used to produce high-quality lettuce (Lactuca sativa) in locations where production conditions are not optimal for this cool-season crop. To learn what additional benefits shading provides if heat-tolerant cultivars are used and to understand the effects of shading on growth, sensory quality, chemical content, and transcriptome profile on heat-tolerant lettuce, we grew two romaine lettuce cultivars with and without shading using 50% black shadecloth in 2018 and 2019. Shading reduced plant leaf temperatures, lettuce head fresh weights, glucose and total sugars content, and sweetness, but not bitterness, whereas it increased lettuce chlorophyll b content compared with unshaded controls. Transcriptome analyses identified genes predominantly involved in chlorophyll biosynthesis, photosynthesis, and carbohydrate metabolism as upregulated in unshaded controls compared with shaded treatments. For the tested cultivars, which were bred to withstand high growing temperatures, it may be preferable to grow them under unshaded conditions to avoid increased infrastructure costs and obtain lettuce deemed sweeter than if shaded.

Will coral reefs survive by adaptive bleaching?

Some reef-building corals form symbioses with multiple algal partners that differ in ecologically important traits like heat tolerance. Coral bleaching and recovery can drive symbiont community turnover toward more heat-tolerant partners, and this ‘adaptive bleaching’ response can increase future bleaching thresholds by 1–2°C, aiding survival in warming oceans. However, this mechanism of rapid acclimatization only occurs in corals that are compatible with multiple symbionts, and only when the disturbance regime and competitive dynamics among symbionts are sufficient to bring about community turnover. The full scope of coral taxa and ecological scenarios in which symbiont shuffling occurs remains poorly understood, though its prevalence is likely to increase as warming oceans boost the competitive advantage of heat-tolerant symbionts, increase the frequency of bleaching events, and strengthen metacommunity feedbacks. Still, the constraints, limitations, and potential tradeoffs of symbiont shuffling suggest it will not save coral reef ecosystems; however, it may significantly improve the survival trajectories of some, or perhaps many, coral species. Interventions to manipulate coral symbionts and symbiont communities may expand the scope of their adaptive potential, which may boost coral survival until climate change is addressed.

Performance of Heat Tolerant Maize Genotypes under Moisture Stress during Rainy Season in Karnataka

This research was formulated to study the response of heat tolerant genotypes to prolonged period of water stress prevalent under rainfed situation. The trial was conducted at Agricultural Research Station, Dhadesugur, University of Agricultural Sciences, Raichur, Karnataka, India, during rainy season (kharif) 2019. The present study comprised of three stress tolerant genotypes (RCRMH 2, RCRMH 3 and RCRMH 4) with four moisture stress stages (imposed between 20-40, 40-60, 60-80 and 80-100 DAS) which were sown during June, July and August, and the design employed was Randomized complete block design. The results revealed that superior performance of RCRMH 3 (5321 kg ha-1) over other genotypes whether stressed or not, but all the genotypes were on par with each other in terms of physiological parameters viz., proline accumulation, relative water content, canopy temperature, NDVI, relative chlorophyll content and ASI, and these parameters exhibited good correlation with yield and hence found ideal for stress studies. The present study indicated that these cultivars sustain maize production under moisture stress condition and therefore, are suitable to semiarid region characterized by inclement weather.