muench method
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Author(s):  
Paulo Roberto Barros Gomes ◽  
◽  
Silvio Carvalho Marinho ◽  
Gustavo Oliveira Everton ◽  
Eduardo Fonseca Silva ◽  
...  

In this study, we investigated the main constituent, the predominant class and biological activity of the essential oil extracted from the leaves of Pimenta dioica and the pattern of the major constituent against larvae in the third stage of Aedes aegypti. For this reason, we extracted the oil by hydrodistillation, identified its components by gas chromatography coupled with mass spectrometry (GC/MS) and calculated the lethal concentration (LC50) of the larvicidal activity using the Reed-Muench method. The results show that the oil consists mainly of eugenol, in which the phenylpropanoid class predominated and the lethal concentration, LC50, was 38.86 μg mL-1at a confidence level of 2.25 μg mL-1, while the eugenol standard presented LC5079.75 μg mL-1at a confidence level of 2.10 μg mL-1. Given the facts, we conclude that the oil is more active than the standard and that it has the potential to replace chemical larvicides.


2021 ◽  
Vol 10 (1) ◽  
pp. e35710111166
Author(s):  
Thércia Gabrielle Teixeira Martins ◽  
Paulo Victor Serra Rosa ◽  
Mariana Oliveira Arruda ◽  
Andressa Almeida Santana Dias ◽  
Ari Pereira de Araújo Neto ◽  
...  

This study aimed to evaluate the larvicidal activity of essential oil microparticles (EO) of Melissa officinalis L. against Aedes aegypti. The leaves of M. officinalis were collected in the municipality of São José de Ribamar, Maranhão, Brazil, later dried, crushed and ground. 90g of the dried leaves were used to obtain the EO by the hydrodistillation method. For the synthesis of microencapsulated EO, 60g of sodium alginate (2.5% m/v) was added to the mixture of 15g of Tween 20 with 6g of EO. The mixture was homogenized and drips over CaCl2 5% m/v solution for the hardening of particles via crosslinking. The microparticles were washed with distilled water in filter and dried at 35ºC/24h and 15 days at tamb (30ºC). The eggs of Aedes aegypti were collected at the Federal University of Maranhão by the ovitrampas method. The larvae that hatched were fed until they reached the fourth instar. Groups of larvae (n=20) were submitted to solutions of EO and microparticles of 10-90 mg/L . After 24 h, live and dead larvae were counted and LC50 was calculated by the Reed&Muench method, using Cheng's criterion for classification of active potential. All larvae presented mortality in all concentrations tested. The LC50 obtained for the EO was 40.60 mg/L and for the microparticles 22.10 mg/L, both classified as active according to the adopted criterion, but it is observed that the microparticles increased the larvicidal potential of the EO. Through the results obtained, it is concluded that the microparticles formulated with the EO proved to be efficient in the face of the larvae of Aedes aegypti, being interesting and important in controlling and combating the mosquito that transmits dengue.


Author(s):  
Mikhail Semenovich Tikhov ◽  
◽  
Ksenia Nikolaevna Shkileva ◽  

2019 ◽  
Vol 31 (4) ◽  
pp. 616-619 ◽  
Author(s):  
Erica Spackman ◽  
Sasidhar Malladi ◽  
Amos Ssematimba ◽  
Christopher B. Stephens

Embryonating chicken eggs (ECEs) are among the most sensitive laboratory host systems for avian influenza virus (AIV) titration, but ECEs are expensive and require space for storage and incubation. Therefore, reducing ECE use would conserve resources. We utilized statistical modeling to evaluate the accuracy and precision of AIV titration with 3 instead of 5 ECEs for each dilution by the Reed–Muench method for 50% endpoint calculation. Beta-Poisson and exponential dose-response models were used in a simulation study to evaluate observations from actual titration data from 18 AIV isolates. The reproducibility among replicates of a titration was evaluated with one AIV isolate titrated in 3 replicates with the beta-Poisson, exponential, and Weibull dose-response models. The standard deviation (SD) of the error between input and estimated virus titers was estimated with Monte Carlo simulations using the fitted dose-response models. Good fit was observed with all models that were utilized. Reducing the number of ECEs per dilution from 5 to 3 resulted in the width of the 95% confidence interval increasing from ±0.64 to ±0.75 log1050% ECE infectious doses (EID50) and the SD of the error increased by 0.03 log10EID50. Our study suggests that using fewer ECEs per dilution is a viable approach that will allow laboratories to reduce costs and improve efficiency.


2013 ◽  
Vol 662 ◽  
pp. 417-423
Author(s):  
Hao Huang ◽  
Zhong Hua Yang ◽  
Jun Chen

Glucomannan extracted from konjac was sulfated (konjac oligo-glucomannan sulfate, OGMS) to show selective anti- coxsackievirus B activity in vitro using Hela cell line as the host cell of coxsackievirus B. OGMS straight inhibited the virus according to different groups and CVB-RNA Replication in Hela cell by RT-PCR. The studies indicated that OGMS mainly acted as blocking virus invading function and its IC50 was 0.1483 mg/mL determined by Reed-Muench method and CVB-RNA Replication was inhibited as well. All these indicated that OGMS had evident in vitro anti- coxsackievirus B activity.


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