dual regulator
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2021 ◽  
pp. 103917
Author(s):  
Eliana Alcaraz ◽  
Barbara Ghiglione ◽  
María Verónica Pineda ◽  
Andrea Mangano ◽  
José Di Conza ◽  
...  

Author(s):  
Shoib Mohmad Wani ◽  
Adil Gani ◽  
F.A. Masoodi ◽  
Sajad A. Mir ◽  
F.A. Khanday

Author(s):  
Shicong Zhao ◽  
Sizhu Pei ◽  
Ailu Wang ◽  
Yu Chen ◽  
Peng Zhang ◽  
...  

Author(s):  
Ryo Muko ◽  
Hiroshi Matsuda ◽  
Masa-aki Oikawa ◽  
Taekyun Shin ◽  
Kenshiro Matsuda ◽  
...  

2020 ◽  
Author(s):  
Niklas Tenhaef ◽  
Robert Stella ◽  
Julia Frunzke ◽  
Stephan Noack

Molecular cloning is the core of Synthetic Biology, as it comprises the assembly of DNA and its expression in target hosts. At present, however, cloning is most often a manual, time-consuming and repetitive process that highly benefits from automation. The automation of a complete rational cloning procedure, i.e., from DNA part creation to expression in the target host, involves the integration of different operations and machines. Examples of such workflows are sparse, especially when the design is rational (i.e., the DNA sequence design is fixed, and not based on randomized libraries) and the target host is less genetically tractable (e.g., not sensitive to heat-shock transformation). In this study, an automated workflow for the rational construction of plasmids and their subsequent conjugative transfer into the biotechnological platform organism Corynebacterium glutamicum is presented. The whole workflow is accompanied by a custom-made software tool. As an application example, a rationally designed library of transcription factor biosensors based on the regulator Lrp was constructed and characterized. A sensor with an improved dynamic range was obtained, and insights from the screening provided evidence for a dual regulator function of C. glutamicum Lrp.


2020 ◽  
Vol 21 (21) ◽  
pp. 7929
Author(s):  
Kristi Moncja ◽  
Michael W. Van Dyke

Transcription factors (TFs) have been extensively researched in certain well-studied organisms, but far less so in others. Following the whole-genome sequencing of a new organism, TFs are typically identified through their homology with related proteins in other organisms. However, recent findings demonstrate that structurally similar TFs from distantly related bacteria are not usually evolutionary orthologs. Here we explore TTHB099, a cAMP receptor protein (CRP)-family TF from the extremophile Thermus thermophilus HB8. Using the in vitro iterative selection method Restriction Endonuclease Protection, Selection and Amplification (REPSA), we identified the preferred DNA-binding motif for TTHB099, 5′–TGT(A/g)NBSYRSVN(T/c)ACA–3′, and mapped potential binding sites and regulated genes within the T. thermophilus HB8 genome. Comparisons with expression profile data in TTHB099-deficient and wild type strains suggested that, unlike E. coli CRP (CRPEc), TTHB099 does not have a simple regulatory mechanism. However, we hypothesize that TTHB099 can be a dual-regulator similar to CRPEc.


2020 ◽  
Vol 30 (18) ◽  
pp. 3664-3671.e4
Author(s):  
Kuo-Fu Tseng ◽  
Keith J. Mickolajczyk ◽  
Guangxi Feng ◽  
Qingzhou Feng ◽  
Ethiene S. Kwok ◽  
...  
Keyword(s):  

Cytoskeleton ◽  
2020 ◽  
Vol 77 (3-4) ◽  
pp. 76-83 ◽  
Author(s):  
Rita Pinto‐Costa ◽  
Mónica M. Sousa

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