MYB transcription factor family in sweet cherry (Prunus avium L.): genome-wide investigation, evolution, structure, characterization and expression patterns

Back ground MYB Transcription factors (TFs) are most imperative and largest gene family in plants, which participate in development, metabolism, defense, differentiation and stress response. The MYB TFs has been studied in various plant species. However, comprehensive studies of MYB gene family in the sweet cherry (Prunus avium L.) are still unknown. Results In the current study, a total of 69 MYB genes were investigated from sweet cherry genome and classified into 28 subfamilies (C1-C28 based on phylogenetic and structural analysis). Microcollinearity analysis revealed that dispersed duplication (DSD) events might play an important role in the MYB genes family expansion. Chromosomal localization, the synonymous (Ks) and nonsynonymous (Ka) analysis, molecular characteristics (pI, weight and length of amino acids) and subcellular localization were accomplished using several bioinformatics tools. Furthermore, the members of distinct subfamilies have diverse cis-acting regions, conserved motifs, and intron-exon architectures, indicating functional heterogeneity in the MYB family. Moreover, the transcriptomic data exposed that MYB genes might play vital role in bud dormancy. The quantitative real-time qRT-PCR was carried out and the expression pattern indicated that MYB genes significantly expressed in floral bud as compared to flower and fruit. Conclusion Our comprehensive findings provide supportive insights into the evolutions, expansion complexity and functionality of PavMYB genes. These PavMYB genes should be further investigated as they seem to be brilliant candidates for dormancy manipulation in sweet cherry.

Effects of foliar Application with Zinc and Boron On fruitset and yield of Sweet cherry (Prunus avium L. var. Bing): تأثير الرش بالزنك والبورون في نسبة العقد والإنتاج للكرز الحلو (Prunus avium L. var. Bing)

The study aims to increase the fruitset percentage of sweet cherry trees, reduce their fall rate and increase fruit retention percentage that reaches the maturity stage. It was conducted during 2019/2020 years at Cherry orchard located in Sargaya- Al- Zabadani area in Rural Damascus, in Syria. the experiment included 4 foliar spray treatments (T1: Control, T2: Zn (100 ppm), T3: B (500 ppm), T4: (100 ppm Zn + 500 ppm B) on sweet cherry trees (Prunus Avium L.) cultivar (Bing) the fruitset percentage, fruit drop percentage, fruiting factor, and yield quantity were calculated for all treatments. The results showed that all treatments (T2, T3, T4) recorded higher fruitset percentage, compared to the control (T1) with no significant differences between (74.83, 76.35, 76.25%) respectively, while the control fruitset percentage (72.76%), and (T4) has achieved the highest percentage of fruiting factor (41.40%) with no significant differences between it and treatment (T3) (37.12%), and the highest yield (19.98 kg), as well as (T2, T3) treatments was (9.39, 10.80 kg/tree) respectively, while the control yield was (5.93 kg/tree). Therefore, it can be considered that treatment (T4) has succeeded in reducing Sweet cherry fruit drop, where the fruit drop percentage didn't exceed (70.27%), and in (T2, T3) treatments was (74.94, 72.99%) respectively, while it reached in the control treatment to (80.64%).

A new genus Bongotarsonemus with two new species of tarsonemid (Acari: Heterostigmata) mites discovered from the Himalayan forests of West Bengal, India

Bongotarsonemus unicornus gen. nov., sp. nov. (Tarsonemidae: Tarsoneminae) is described from adult females and males collected on leaves of Sweet Cherry, Prunus avium L. (Rosaceae). Another species, Bongotarsonemus bicornus sp. nov. is described from adult females collected on leaves of a mountain fern Arthromeris wallichiana (Spreng.) Ching (Polypodiaceae). Both species were collected from the Himalayan forests of Darjeeling district in the State of West Bengal, India. Adult females of this new genus are unusual in having a median raised section of the prodorsum which is delimited by elongated tubercles bearing setae v1 and sometimes sc2. This area is ornamented with sclerotized ridges or elevated strial thickenings; and has a single or bifurcated spear-shaped horn between bases of setae v1. The new genus also has a characteristically bipartite sejugal apodeme and seta d on tibia I often reaching beyond the claw. Adult males are characterized by seta sc2 subequal to sc1; tarsus II with baciliform seta pl'' longer than half the length of the segment, and a conspicuously enlarged solenidion on tarsus IV. Both sexes are unique in having a fish-hook shaped claw on leg I with an elongated shank and strongly curved apex, and seta l' on femur I plumose.

BIODIVERSITY OF FRUIT-BEARING FOREST SPECIES IN THE PROTECTED LANDSCAPE "KONJUH"

UDK: 634.2:574.4]:502(497.6 “Konjuh“) The area of the protected landscape "Konjuh" is distinguished by quality forest ecosystems, featuring diverse fruit-bearing forest species, which contributes to the conservation of biodiversity of the protected landscape "Konjuh". The aim of the paper is to assess the state of biodiversity of fruit-bearing species in the forest ecosystems of the study area, with the emphasis on determining the genus richness as a basis for conservation (in/ex situ), breeding and economic use. During the vegetation season in 2015 and 2016, several relevés (phytosociological plot) were made in the Protected Landscape "Konjuh". The results of the research indicate the high level of variability for most of the species according to characteristics of biology, pomology, and ecology. The fruit trees, like the noble hardwood, are wild cherry, (Prunus avium L.), wild pear (Pyrus communis L.), wild apples (Malus sylvestris Mill.), Breccia (Torminalis clusii M. Roem.), Sorbus aria L. et all, Wild cherry, Vrapcarka (Prunus avium L.) is the most famous forest fruit grower, occurs as a single tree or in smaller groups (Noćajević, 2009). The benefits of fruit-bearing forest species are emphasized in the bloom and the fruiting time, when "decorated" forest provide a rich bee pasture and feeding nutriment for forest fauna (Orešković et al. 2006). Fruit-bearing Forest species are significant as the genera of the varieties and as a basis for the grafting for high-grade varieties. Also, they are important for humans from the aspect of ecology, nutrition, dietotherapy, pharmacology and bioenergy balance.

Genome-Wide Identification of ARF Gene Family Suggests a Functional Expression Pattern during Fruitlet Abscission in Prunus avium L.

Auxin response factors (ARFs) play a vital role in plant growth and development. In the current study, 16 ARF members have been identified in the sweet cherry (Prunus avium L.) genome. These genes are all located in the nucleus. Sequence analysis showed that genes in the same subgroup have similar exon-intron structures. A phylogenetic tree has been divided into five groups. The promoter sequence includes six kinds of plant hormone-related elements, as well as abiotic stress response elements such as low temperature or drought. The expression patterns of PavARF in different tissues, fruitlet abscission, cold and drought treatment were comprehensively analyzed. PavARF10/13 was up-regulated and PavARF4/7/11/12/15 was down-regulated in fruitlet abscising. These genes may be involved in the regulation of fruit drop in sweet cherry fruits. This study comprehensively analyzed the bioinformatics and expression pattern of PavARF, which can lay the foundation for further understanding the PavARF family in plant growth development and fruit abscission.