At present, marine scientists employ manual method to estimate the components in coral reef environment, where Coral Point Count with Excel extensions (CPCe) software is used to determine the coral reef components and substrate coverage. This manual process is laborious and time consuming, and needs experts to conduct the survey. In this paper, a prototype for estimating the distribution of sand cover in coral reef environment from still images by using colour extraction methods was introduced. The colour segmentation called delta E was used to calculate the colour difference between two colour samples. Another method used was colour threshold by setting the range of sand colour pixels. The system was developed by using a MATLAB software with image processing toolbox. The developed system was semi-automatic computer-based system that can be used by researchers even with little knowledge and experience to estimate the percentage of sand coverage in coral reef still images.