Microbial inputs at the litter layer translate climate into altered organic matter properties

<p>Plant litter chemistry is altered during decomposition but it remains unknown if these alterations, and thus the composition of residual litter, will change in response to climate. Selective microbial mineralization of litter components and the accumulation of microbial necromass can drive litter compositional change, but the extent to which these mechanisms respond to climate remains poorly understood. We addressed this knowledge gap by studying needle litter decomposition along a boreal forest climate transect. Specifically, we investigated how the composition and/or metabolism of the decomposer community varies with climate, and if that variation is associated with distinct modifications of litter chemistry during decomposition. We analyzed the composition of microbial phospholipid fatty acids (PLFAs) in the litter layer and measured natural abundance δ<sup>13</sup>C<sub>PLFA</sub> values as an integrated measure of microbial metabolisms. Changes in litter chemistry and δ<sup>13</sup>C values were measured in litterbag experiments conducted at each transect site. A warmer climate was associated with higher litter nitrogen concentrations as well as altered microbial community structure (lower fungi:bacteria ratios) and microbial metabolism (higher δ<sup>13</sup>C<sub>PLFA</sub>). Litter in warmer transect regions accumulated less aliphatic‐C (lipids, waxes) and retained more O‐alkyl‐C (carbohydrates), consistent with enhanced <sup>13</sup>C‐enrichment in residual litter, than in colder regions. These results suggest that chemical changes during litter decomposition will change with climate, driven primarily by indirect climate effects (e.g., greater nitrogen availability and decreased fungi:bacteria ratios) rather than direct temperature effects. A positive correlation between microbial biomass δ<sup>13</sup>C values and <sup>13</sup>C‐enrichment during decomposition suggests that change in litter chemistry is driven more by distinct microbial necromass inputs than differences in the selective removal of litter components. Our study highlights the role that microbial inputs during early litter decomposition can play in shaping surface litter contribution to soil organic matter as it responds to climate warming effects such as greater nitrogen availability.</p>

Future Arctic soil nutrient availability and microbial community structure

<p>The Arctic permafrost soils are very diverse in regard to parent material, geobiological composition and genesis. There is sparse knowledge about nutrient availability in Arctic soil and it was found that the permafrost layer differs in nutrient availability compared to the active layer. Recently, it was shown that elements like Si, Ca and P are potentially affecting the greenhouse gas from Arctic soil. However, it is not known how those elements are distributed in Arctic soils for a larger dataset. Furthermore, it is unclear whether regional differences in the availability of those elements or a change in availability due to permafrost thaw is changing microbial decomposer community. Therefore, we analyzed 445 soil depth profiles around the Arctic regarding different element availabilities.</p><p>Furthermore, we conducted an incubation experiment to measure the effect of different Si, Ca and P availabilities on the structure of the microbial decomposer community. We found large differences in the availability of Si, Ca, Al, Fe and P in the layers of the panarctic permafrost soils from Canada, Alaska, Russia, Scandinavia, Greenland and Svalbard. There are differences in the distribution of Ca and Si pools over the panarctic permafrost soils. Especially the availability of P is directly linked to the concentration of Ca and Si and the presence of Al and Fe based minerals. With rising temperatures, the thaw depth of the upper horizon may increase and elements stored in deeper layers become potentially mobilized. These processes modify the nutrient availability for microorganisms and by this the production of greenhouse gases like CO<sub>2</sub> and CH<sub>4</sub>.</p><p>The community structure of bacteria and fungi is related to the availability of Ca and Si. With modified availabilities of Si and Ca, we found direct linear correlations in the changes of the microbial structure at the phylum level for Greenlandic soils. These changes depend on the origin of the soil and the original availability of Ca and Si. We found direct links between the share of gram-positive bacteria and the Ca concentration in both soils and the production of greenhouse gases. The availabilities of these elements may be helpful for better predicting greenhouse gases fluxes in the Arctic as well as element transfer to marine systems.</p>