Rapid, high throughput library preparation of SARS-CoV2 using Illumina’s DNA Prep Library Preparation Kit v2

This procedure provides instructions on how to prepare DNA libraries for whole genome sequencing on an Illumina MiSeq or NextSeq using Illumina’s DNA Prep Library Preparation Kit scaled to half reaction volumes with modifications to the post-PCR procedures; tagmentation stop buffer and associated washes are removed and libraries are pooled post PCR then a single size selection is performed. This protocol is used to sequence SARS-CoV-2 using the cDNA/PCR protocol: https://dx.doi.org/10.17504/protocols.io.b3viqn4e

Rapid, high throughput library preparation of SARS-CoV2 using Illumina’s DNA Prep Library Preparation Kit v2

This procedure provides instructions on how to prepare DNA libraries for whole genome sequencing on an Illumina MiSeq or NextSeq using Illumina’s DNA Prep Library Preparation Kit scaled to half reaction volumes with modifications to the post-PCR procedures; tagmentation stop buffer and associated washes are removed and libraries are pooled post PCR then a single size selection is performed.

Impact of cloud base turbulence on CCN activation: Single size CCN

This paper examines the impact of cloud-base turbulence on activation of cloud condensation nuclei (CCN). Following our previous studies, we contrast activation within a non-turbulent adiabatic parcel and an adiabatic parcel filled with turbulence. The latter is simulated by applying a forced implicit large eddy simulation within a triply periodic computational domain of 643 m3. We consider two monodisperse CCN. Small CCN have a dry radius of 0.01 micron and a corresponding activation (critical) radius and critical supersaturation of 0.6 micron and 1.3%, respectively. Large CCN have a dry radius of 0.2 micron and feature activation radius of 5.4 micron and critical supersaturation 0.15 %. CCN are assumed in 200 cm−3 concentration in all cases. Mean cloud base updraft velocities of 0.33, 1, and 3 m s−1 are considered. In the non-turbulent parcel, all CCN are activated and lead to a monodisperse droplet size distribution above the cloud base, with practically the same droplet size in all simulations. In contrast, turbulence can lead to activation of only a fraction of all CCN with a non-zero spectral width above the cloud base, of the order of 1 micron, especially in the case of small CCN and weak mean cloud base ascent. We compare our results to studies of the turbulent single-size CCN activation in the Pi chamber. Sensitivity simulations that apply a smaller turbulence intensity, smaller computational domain, and modified initial conditions document the impact of specific modeling assumptions. The simulations call for a more realistic high-resolution modeling of turbulent cloud base activation.

Scour downstream of a corrugated apron under wall jets

Experiments were performed over smooth and corrugated aprons with different corrugation dimensions to study the scour and flow characteristics under submerged wall jets condition. The scour depth and length are significantly lower for corrugated than smooth rigid aprons. The maximum reductions in scour depth and length are 79 and 83%, respectively. Optimum scour depth and length are found for aspect ratio (ratio of corrugation wave length to amplitude) three for corrugated apron. The factors affecting scour depth and length were analyzed graphically, and empirical equations are proposed for predicting maximum scour depth and length, and the point of maximum scour depth for corrugated aprons. Velocity, turbulence characteristics, and Reynolds stress in scour holes for smooth and corrugated aprons were also studied. HIGHLIGHT This paper presents the scour downstream of corrugated apron and flow characteristics under submerged wall jets. Here scour depth and length reduces significantly than other apron. In this we have tried to develop empirical equation on single size sediment considering all the flow parameter and apron parameter. Besides this we have also conducted study related to turbulence and shear stress and velocity vector profile.

A Review on Chemical Synthesis and Density Enhancement in HAp / TiO2 Implants

Owing to the excellent mechanical properties , good strength , low density and low toxicity in body fluid, most implants used are based on titanium and its alloys. They don't really, though, have high conductivity and osteo-integration. When using biocompatible and bioactive coatings, the compatibility and bioactivity of the implant material can be boosted. HAp, which is commonly evaluated for its chemical inertness and osteoinduction, is among the most used coating materials that meet the above requirements. For good clinical results, significant scientific findings, and a clear regulatory pathway, research and development of porous structures continues. Although not all applications suit a single size distribution or patterned structure, the majority of porous and textured biomaterials used in medical devices share the common feature of interlinking spaces that facilitate the transfer of nutrients and facilitate the differentiation and proliferation of cells.Safe graft interaction with the surrounding host cell decreases micromotion-induced inflammation and supports steady growth of fibrous content that facilitates healing and reduces disease. So far, many porogenous materials such as napthelene and camphor have been used to establish porosity. When exposed to high temperature, these porogenes will escape from the material that causes Inter communication pores in the material. Hydroxyapatite ( HAp) belongs to a biologically active moving charges which provides a solid link with the tissues of the surrounding body. One of this material 's drawbacks is that it has lower strength. Titanium oxide (TiO2) is a metal that belongs to the reinforcing agents material class and does not respond to the tissue surrounding it.TiO2's tensile characteristics is usually high and, as an implant unit, can be a good replacement. Efforts have been made to make a combination with better surface quality of both HAp / TiO2 biomaterial device and porosity HAp is commonly used as an implant for the reconstruction of bones, as a covering for metal substrates and as a drug-controlled release. Keywords: Hydroxyapatite, Biocompatibility, Bioactive, Bioinert, Porosity

A redescription of Stenopora and the type species Stenopora tasmaniensis Lonsdale, 1844 (Trepostomata, Bryozoa)

Type material for Stenopora tasmaniensis Lonsdale, 1844 was lost in the late nineteenth century, and subsequent descriptions of the genus have been based on material incorrectly assigned to the type species. A neotype is erected for S. tasmaniensis from the original type locality and the genus redescribed. The genus exhibits ramose, frondescent, encrusting, and massive colony morphologies, diaphragms are absent, and acanthostyles of a single size surround each aperture. This single size of acanthostyles aligns with the original type species description; however, it differs from the subsequently accepted genus description and may result in existing species being removed from the genus. Analysis of zooecial characters of a single colony exhibiting both frondescent and ramose morphologies reveals statistically significant differences between subsampled sections, despite being from the same colony. Differences relate to details of zooecial parameters and are not controlled by colony morphology. This variation within a single colony confirms the importance of using qualitative characters alongside quantitative measures in defining Paleozoic bryozoan species.

Experimental Study of the Aggregate Shapes in Self-Compaction

Compaction operations have a vital role in embankments or rock fills to avoid settlement, but in some projects, such as marine ones, it is hardly possible to accomplish compaction operations due to the problems and executive limitations. In situations with no possibility of compaction, it is recommended to use single-size or self-compacted materials. From a theoretical point of view, self-compacted materials consist of coarse aggregates with no vast domain of gradation. In this case, the porosity of the materials in the dense state is not significantly different from the loose one, and a relatively dense condition occurs after it is poured; thus, the mass of materials will undergo lower volumetric changes in the future. In this study, the self-compacted characteristic of materials has been investigated using real aggregates with different gradations (the ratio of the largest to the smallest aggregate size of 1, 2, 4, and 8). The gradation and shape of aggregates are the main variables examined in the research. Real aggregates have been used in order to compare the study of self-compacted idea with ideal aggregates and the effects of sphericity and angularity of them. According to the experiments carried out on samples in the present work, it was observed that, without compaction operations, even ideal materials would not be in fully self-compacted state. However, relatively denser conditions can be achieved by observing the necessary points. Moreover, aggregates with high sphericity have better self-compacted property. Furthermore, the more uniform gradation and bigger size of materials lead to more self-compacted pile of materials.

Method of kurtosis in estimating the measurement uncertainty during calibration of the electrical resistance measures using a potentiometer

Calibration of electrical resistance measures is considered by the indirect method, which is realized through measuring the voltage drop across the series-connected reference and calibrated resistors. The biases of the estimates of the measurand and the combined standard uncertainty due to the nonlinearity of the measurement model were calculated. The distribution laws of the input quantities in the calculation of the expanded uncertainty were taken into account by the kurtosis method. An example of measurement uncertainty evaluating during calibration of single-size electrical resistance measure R331 with a nominal resistance of 1000 Ω by comparing its value using a potentiometer R345 with the value of the calibrated reference standard is considered. Estimates of the measurand and its standard and expanded uncertainties obtained using the proposed method showed good agreement with the estimates obtained using the Monte Carlo method.

The Precision Benefit

This chapter offers the basic justification for personalized law: the precision benefit. Personalized rules could accomplish the underlying goals of any law more effectively. Any goals, of any law. The reason is almost trivial: personalized law accounts for more relevant circumstances and differences in tailoring individual commands. This is the same reason that custom-made shoes fit better than single size, or that medicine based on personalized diagnostics cures better than one-size-fits-all treatments. Uniform rules and commands, even if optimal on average, are a poor fit for people with diverse preferences, characteristics, histories, and means. The chapter begins by demonstrating the value of personalization in other sectors of human activity, like medicine and education, and then uses an example of data protection law to display the benefits of personalized rules. It also discusses the production costs of personalized law, primarily the costs associated with the use of more information in the promulgation of legal commands.