Stable Isotope and Radiocarbon Analysis for Diet, Climate and Mobility Reconstruction in Agras (Early Iron Age) and Edessa (Roman Age), Northern Greece

In this article we present an isotopic analysis of human bone collagen (δ13Ccol, and δ15Ncol) and bone apatite (δ13C) for diet reconstruction, as well as δ18Oap of human bone apatite for climate reconstruction, using samples from Northern Greece. Radiocarbon dating analysis was conducted on three of the Agras samples and the results (from 1000 to 800 BC) correspond to the Early Iron Age. Isotopic values for δ13Ccol range from −20.5‰ to −16‰ and for δ15Ncol from 6‰ to 11.1‰—a strong indication of a C3-based diet, with contributions by C4 and freshwater fish elements. The results were compared to the ones from Roman Edessa, and Alexandreia (a contemporary city near Edessa), as well as to other Early Iron sites in Greece and wider Europe. In general, the results from Agras are in good agreement with the results from Northern Greece, with the exception of the Makriyalos site, and are quite close to those of Croatia’s and Hungary’s sites. Additionally, from the δ18Oap results we calculated the oxygen isotopic composition of consumed water for Agras (from −9.6‰ to −10.9‰) and for Roman Edessa (from −9.6‰ to −11.2‰) for the palaeoclimate and palaeomobility reconstruction.

These boots are made for burnin’: Inferring the position of the corpse and the presence of leather footwears during cremation through isotope (δ13C, δ18O) and infrared (FTIR) analyses of experimentally burnt skeletal remains

Cremation is a complex mortuary practice, involving a number of activities of the living towards the dead before, during, and after the destruction of the bodily soft tissues by fire. The limiting information concerning these behavioral patterns obtained from the pyre remains and/or cremation deposits prevents the reconstruction of the handling of the corpse during the burning process. This pioneering study tries to determine the initial positioning of the corpse in the pyre and assess whether the deceased was wearing closed leather shoes during cremation through isotopic (δ13C, δ18O) and infrared (ATR-FTIR) analyses of experimentally burnt pig remains, used as a proxy for humans. The results obtained show that both the position of feet on or within the pyre and the presence of footwears may moderately-to-highly influence the oxygen isotope ratios of bone apatite carbonates and the cyanamide content of calcined bone in certain situations. By forming a protective layer, shoes appear to temporarily delay the burning of the underlying pig tissues and to increase the heat-shielding effect of the soft tissues protecting the bone mineral fraction. In such case, bioapatite bone carbonates exchange oxygen with a relatively more 18O-depleted atmosphere (due to the influence of lignin-derived oxygen rather than cellulose-derived oxygen), resulting in more pronounced decrease in the δ18Ocarb values during burning of the shoed feet vs. unshoed feet. The shift observed here was as high as 2.5‰. A concomitant isotopic effect of the initial location of the feet in the pyres was also observed, resulting in a top-to-bottom decrease difference in the δ18Ocarb values of shoed feet of about 1.4‰ between each deposition level tested. Finally, the presence of cyanamide (CN/P ≥ 0.02) seems to be indicative of closed footwear since the latter creates favorable conditions for its incorporation into bone apatite.

The Coating of Bovine-Source Hydroxyapatite on Titanium Alloy (Ti-6Al-4V ELI) Using Electrophoretic Deposition for Biomedical Application

Research about the utilization of titanium alloy (Ti-6Al-4V ELI) as implant material in the treatment of orthopedic cases had been increasing. Health problems appear due to the drawbacks of using titanium. The lack of titanium using is bio-inertness characteristic, which decreasing its bioactivity and results in low bone growth and effect for implant failure. The titanium can be modified with coating on the surface using a bioactive substance that is natural-source hydroxyapatite. Bovine-source hydroxyapatite (bovineHA) contains apatite component that is similar to human bone apatite. The coating process was carried out using particle size variation (25 μm, 63 μm, and 125 μm) of bovineHA. The electrophoretic deposition (EPD) method was applied to coat hydroxyapatite with 10 volt for 5 minutes onto the titanium surface. The result showed that different size particles have an effect on coating properties. The coating composed by particle-sized 25 μm has better surface coverage (95.89%), indicating more particle mass (particle weight 6.97x103 μg) attached to surface material, thus resulting thick coating. The good coating characteristic using bovine-source hydroxyapatite with small particle size was expected can be used in biomedical applications due to fulfill the prerequisite of the bone implant.

Bayesian Modeling of Wood-Age Offsets in Cremated Bone

ABSTRACTExperimental studies have shown that significant carbon exchange occurs between bone-apatite and the pyre atmosphere during cremation, which can cause a calendar date offset between the radiocarbon (14C) event and the date of cremation. There are limited empirical data available to assess the magnitude of such wood-age offsets, but the aim of this paper is to test if they can be modeled statistically. We present new 14C dates on modern bone cremated in realistic open-air experiments and on archaeological samples of cremated bone and associated organic material. Experimental results demonstrate a wide range of carbon exchange with a mean of 58.6 ± 14.8%. Archaeological results indicate that the wood-age offsets have an approximately exponential distribution. We test whether the default Charcoal Outlier_Model in OxCal v4.3, developed to reduce the impact of wood-age offsets in dates of charcoal, is appropriate for cremated bone, but find that it slightly underestimates apparent offsets. To counter the intrinsic age of both pyre fuel and unburned bio-apatite, we instead propose a bespoke Cremation Outlier_Model, which combines an exponential distribution of calendar age offsets with a minimum offset, and provides better estimates of the actual dates of cremations.

Bone apatite anisotropic structure control via designing fibrous scaffolds

Osteoblast alignment on the fibrous scaffolds as well as the subsequent apatite c-axis orientation increased with increasing fiber collecting speed. We successfully controlled cell alignment and apatite c-axis orientation using the designed morphology of fibrous scaffolds.

Design of hydroxyapatite bioceramics with micro-/nano-topographies to regulate the osteogenic activities of bone morphogenetic protein-2 and bone marrow stromal cells

Biomimicking the nanostructure of natural bone apatite to enhance the bioactivity of hydroxyapatite (HA) biomaterials is an eternal topic in the bone regeneration field.

In vivo changes of nanoapatite crystals during bone reconstruction and the differences with native bone apatite

Hydroxyapatite (HA) plays an important role in clinical bone repair. However, it remains a challenge to accurately determine its changes during bone reconstruction and to identify its differences from native bone apatite. Here, terbium (Tb) doped uniform HA nanocrystals were implanted into bone tissue and compared with native bone apatite. These comparisons demonstrated the occurrence of compositional and structural alteration of the implanted HA nanocrystals, and their gradual degradation, during bone reconstruction. They also revealed notable differences between HA nanocrystals and bone apatite crystals in crystal size, distribution pattern, and state of existence in bone tissue. Although synthetic HA nanocrystals could osteointegrate with bone tissue, they still seemed to be treated as foreign material in this tissue and thus were gradually degraded. These findings can help to identify and rethink the function of synthetic apatite and bone apatite, which will benefit future design and application of biomimetic bone repair materials.