Respiratory system compliance at the same PEEP level is similar in COVID and non-COVID ARDS

Background The comparison of respiratory system compliance (Crs) between COVID and non-COVID ARDS patients has been the object of debate, but few studies have evaluated it when considering applied positive end expiratory pressure (PEEP), which is one of the known determinants of Crs itself. The aim of this study was to compare Crs taking into account the applied PEEP. Methods Two cohorts of patients were created: those with COVID-ARDS and those with non-COVID ARDS. In the whole sample the association between Crs and type of ARDS at different PEEP levels was adjusted for anthropometric and clinical variables. As secondary analyses, patients were matched for predicted functional residual capacity and the same association was assessed. Moreover, the association between Crs and type of ARDS was reassessed at predefined PEEP level of 0, 5, 10, and 15 cmH2O with a propensity score-weighted linear model. Results 367 patients were included in the study, 276 patients with COVID-ARDS and 91 with non-COVID ARDS. The association between Crs and type of ARDS was not significant in both the complete cohorts (p = 0.17) and in the matched cohorts (p = 0.92). This was true also for the propensity score weighted association at PEEP 5, 10 and 15 cmH2O, while it was statistically significant at PEEP 0 (with a median difference of 3 ml/cmH2O, which in our opinion is not clinically significant). Conclusions The compliance of the respiratory system is similar between COVID ARDS and non-COVID ARDS when calculated at the same PEEP level and while taking into account patients’ anthropometric characteristics.

Understanding NO2 Concentration Dynamics within Tema Metropolitan Area of Ghana Using Generalized Linear Model

The concentration of nitrogen dioxide (NO2) is worsening across the globe alongside growth in industrial and general anthropogenic activities. Due to its serious health implications with long-term exposure, studies on NO2 concentration have gained space in the academic literature. In this study, awareness is created on the levels of NO2 across four (4) locations within the Tema Metropolitan area, with specific interest in selecting locations and periods significantly saturated with NO2 within the study area. NO2 was measured using RKI Eagle, an instrument with a built-in sensor for a specific gas measurement. Measurements were taken day and night at sampling points around 100 meters apart in each location. Data collection was performed over a nine (9)-month period. The Generalized Linear model is explored for selecting locations and periods significantly affected by NO2. From the results, the fourth week (26th–31st) of July 2020, the fourth week (27th–31st) of December 2020, the first week (1st–7th) of January 2021, and the fourth week (24th–31st) of January 2021 recorded severe concentrations of NO2. Additionally, the lives of residents in the Oil Jetty and the VALVO hospital areas were found to be the most endangered, as they recorded significantly high concentrations of NO2. In a developing country such as Ghana, this study is useful for monitoring NO2 concentrations in similar areas to inform decision making and environmental policy formulation.

Phenotypic Diversity and Association Mapping of Ascorbic Acid Content in Spinach

Ascorbic acid (AsA), or vitamin C, is an essential nutrient for humans. In plants, AsA functions as an antioxidant during normal metabolism or in response to stress. Spinach is a highly nutritious green leafy vegetable that is consumed fresh, cooked or as a part of other dishes. One current goal in spinach breeding programs is to enhance quality and nutritional content. However, little is known about the diversity of nutritional content present in spinach germplasm, especially for AsA content. In this study, a worldwide panel of 352 accessions was screened for AsA content showing that variability in spinach germplasm is high and could be utilized for cultivar improvement. In addition, a genome-wide association study for marker-trait association was performed using three models, and associated markers were searched in the genome for functional annotation analysis. The generalized linear model (GLM), the compressed mixed linear model (CMLM) based on population parameters previously determined (P3D) and the perMarker model together identified a total of 490 significant markers distributed across all six spinach chromosomes indicating the complex inheritance of the trait. The different association models identified unique and overlapping marker sets, where 27 markers were identified by all three models. Identified high AsA content accessions can be used as parental lines for trait introgression and to create segregating populations for further genetic analysis. Bioinformatic analysis indicated that identified markers can differentiate between high and low AsA content accessions and that, upon validation, these markers should be useful for breeding programs.

Nonlinear Modeling of Dynamic Characteristics of Pump-Turbine

Hydropower is a kind of clean energy, which can effectively reduce the consumption of fossil energy and is one of the main fields of new energy development. Pumped storage power station not only provides a solution for storing electric energy and generating excess, but also is a clean, efficient, economical and safe power system regulation method with high quality. Accurate modeling of a pump-turbine, as the core equipment of a pumped storage unit, is the key to safe and stable operation of the pumped storage unit. At present, a method of simplifying the external characteristics of a pump-turbine into a first-order linear model is widely used, which cannot effectively and accurately reveal the nonlinear dynamic characteristics of the unit in transition process. In order to meet the demand of high-precision simulation of the unit, a new method of identifying Taylor series expansion considering nonlinearity based on the torque characteristic formula and the flow characteristic formula is proposed, which is applied to the pump-turbine external characteristic model, and retains the second derivative term, making the model a second-order nonlinear model, and thus, the pump-turbine model becomes a nonlinear model. The nonlinear model established is used to simulate the load increase and load rejection of the unit, and the results are compared with those for the linear model. The comparison shows that the nonlinear model established for the pump-turbine can reveal the dynamic response of the unit more effectively and accurately than the linear model, and provide a further guarantee for the safe and stable operation of pumped-storage units, which is of great significance to hydropower energy development.