conventional light microscope
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2020 ◽  
Vol 43 (3) ◽  
pp. 34-40
Author(s):  
Pakpoom Thintharua ◽  
Permphan Dharmasaroja

Histology is an essential field in the education of medical students, and competent knowledge in histology is very important when studying pathology. Current teaching methods for histology in medical schools involve using a conventional light microscope (CM) with or without a virtual microscope (VM). This review aims to present advantages and disadvantages of using CM and VM in terms of teaching and learning histology in the context of undergraduate medical education. One major advantage of the traditional CM histology learning method in laboratory practice is that this allows students to practice using a light microscope; however, study flexibility is limited as the students cannot take the microscope back home for self-study after the histology class has finished. Costly repairs and maintenance must also be considered when using CM. By contrast, VM technology can provide flexibility and convenience for both students and staffs. This method allows students to both self-study and group-study almost anywhere at any time. This review emphasizes that histology learning in undergraduate medical education using VM is no longer confined to the classroom. However, the basic skill of how to operate a conventional light microscope is still important for medical students because CM is commonly used in the hospital laboratories and some hospitals may not be equipped with VM technology.  


2018 ◽  
Author(s):  
A S M Waliullah

Objectives: To check the feasibility of using mobile phone-based microscopy for various types of human histopathological sample investigations. Methodology: A feasibility study was performed by imaging several histopathological samples with one novel type of microscope ‘Foldscope’ and image compared with a conventional microscope in the laboratory facility. The image acquired from both sources were edited further and put together for comparison. Results: Mobile phone-based microscope acquired images were observed and compared with a conventional microscope and found morphology of the tissue sections were significantly similar as of conventional light microscope images. Conclusion: By comparing the image of some non-human histopathological sample, it could be stated that this method is also feasible for human histopathological sample investigations, especially in the low resource area or in case of emergency situations.


2013 ◽  
Vol 467 ◽  
pp. 16-19 ◽  
Author(s):  
Mehdi Shafei ◽  
Najmadin Arab ◽  
Karim Zangeneh Madar

Hypereutectic Al-15Si (wt pct) alloys with various content of rare earth Ce were prepared by conventional casting technique. The influence of the primary silicon phase and eutectic silicon on the solidification process of hypereutectic Al-15Si alloys with the addition of Ce is presented. The microstructures were examined with conventional light microscope and scanning electron microscope. The simultaneous refinement of both the primary and the eutectic silicon particles wasntachieved with Ce additions. The results showed that the addition of cerium doesnt cause to refinement of the primary silicon grains, while cerium is caused to stress on concentration regions.The results showed that the addingthe cerium had a negative influence on the alloys tensile strength.


2001 ◽  
Vol 7 (S2) ◽  
pp. 600-601
Author(s):  
Kathy K. H. Svoboda

Many reagents have been developed recently to label living cells with substrates that will become fluorescent if an enzyme is active. The general principle is that the substrate will be taken up by living cells then detected only if the enzyme is active. These substrates work well with isolated individual cells, however, more difficulty can be encountered when studying whole tissues. Problems can range from substrate penetration into whole tissues to being able to detect the label effectively. We have used the chicken corneal epithelia for many studies, but the tissue is to thick to view with a conventional light microscope, therefore we have developed techniques using laser confocal microscopes to view this tissue in with a variety of techniques including in situ hybridization, immunohistochemistry, and vital dyes/stains.Whole embryonic corneal epithelial sheets can be isolated without the basal lamina.


Author(s):  
A. Boyde

It has been proposed that an overriding advantage of the “black-box” configuration of confocal scanning laser microscope (CSLM) is that it enables the use of all the familiar and conventional light microscope (LM) modes to discover a location of interest in a prepared, contrived, biological sample and then to switch to the confocal mode when desired. By implication, it has been assumed that this would not be possible in the Tandem Scanning Reflected Light Microscope (TSM). It is shown here that such suppositions are incorrect, and that correlations with different LM modes are very easily achieved in standard TSMs. In fact, because of the slow scanning speed of existing commercial CSLMs, it is not just an advantage to have other modes of image formation available, but a pure necessity, since finding the area of interest would otherwise be too difficult.


Author(s):  
G. Schatten ◽  
S. Paddock ◽  
P. Cooke ◽  
J. Pawley

Confocal microscopy holds great promise for improved imaging of fluorescent or reflective biomedical specimens. The IMR is actively investigating the advantages and optimal usage of the Medical Research Council's Lasersharp laser - scanning confocal microscope and Tracor/Northern's Tandem Scanning Microscope, which benefits from the principles outlined by Petran et al. and Boyde.Quantitative evaluation of microscopic images has always been complicated by the effect of out-of-focus structures on the final image. These effects can be greatly reduced if the conventional light microscope is replaced by a scanning-confocal light microscope. In such an instrument two conditions are met: 1) only a single point of the sample is illuminated at any time and 2) this point on the sample is then imaged onto the pinhole at the entrance to the photodetector. Because little light from out-of-focus planes will pass through the pinhole, only in-focus data is recorded.


Author(s):  
W. H. Abbott ◽  
C. O. Pollard

Frustules of the freshwater diatom Melosira granulata (Ehr.) Ralfs from a freshwater diatomite of Miocene age and recent frustules of this species from Reelfoot Lake, Tennessee were examined under a scanning electron microscope to determine fine structure generally not seen or poorly seen with the conventional light microscope.The terminology used to describe frustule structure in this study was originated by Hendy.Melosira granulata generally grows in chains of two connected frustules with each frustule containing two valves or halves. The connection between frustules is accomplished by the encasing of the adjacent valves of two frustules by a structure called the girdle (Fig. 1a).


1967 ◽  
Vol 2 (2) ◽  
pp. 163-168
Author(s):  
J. G. GALL

The analysis of electron micrographs by optical diffraction was introduced recently by Klug & Berger (1964). Their experiments were conducted with a special diffractometer designed for use with diffracting masks up to several inches in diameter. A method is described here for using a conventional light microscope as an optical diffractometer which can accept masks up to 5 mm in diameter. A 100-µ electron-microscope aperture is used as a pinhole source of illumination, and the micrograph to be studied is introduced above the objective. The diffraction pattern produced by the micrograph appears in the usual image plane of the microscope within the eyepiece.


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